Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC

Clinical relevance

Hippuric acid, o-, m-, p-methylhippuric acid, mandelic acid and phenylglyoxylic acid are the main metabolites of the organic solvents: toluene, xylene, and styrene. These toxic compounds are widely used throughout various industries, making the determination of their concentrations relevant to occupational health in a number of different professional fields. Due to their high levels of volatility, they primarily enter the body through inhalation and are rapidly absorbed through the lungs. For occupational health monitoring of people who are exposed to these compounds at work, measurement of the metabolites in urine is more reliable than determining the concentrations of the toxins in the blood, due to their very fast metabolism rates.

For any hazardous substance or its metabolite, a BTV (Biological Tolerance Value) is defined as a threshold value for maximum absorption in biological material. Solvent-related harm to human health can be prevented by performing regular monitoring of urine.

Product advantages

• Only 10 µl sample volume
• Optimised internal standard
• Straightforward sample preparation

This Chromsystems assay provides rapid and reliable determination of the main metabolites of toluene, xylene, and styrene in a single run. A 10 µl urine sample is all that is required, which is then analysed using an isocratic HPLC system with UV detection following a simple and fast preparation step. The inclusion of a tailor-made internal standard reduces analytical variations and guarantees high precision and reliability of results.