Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
Itraconazole/posaconazole analysis upgraded with voriconazole
Tri-level controls available
Custom-made internal standard


Itraconazole
Hydroxyitraconazole
Posaconazole
Voriconazole
Clinical relevance
In recent years there has been an increase in invasive fungal infections due to the growing number of people with immunosuppression. Candida and Aspergillus are the most common types of infection, but previously rare fungal infections such as fusariosis or zygomycosis are also on the rise. Options for oral antimycotic therapy have improved considerably with the advent of new drugs. These include second-generation triazoles such as posaconazole and voriconazole.
Posaconazole is used primarily in case of therapy-resistant mycoses or drug intolerances. Voriconazole is also effective against some itraconazole-resistant Aspergillus species and offers fungicidal activity against emerging fungal pathogens such as Scedosporium or Fusarium. All three azoles exhibit wide inter-individual variability in bioavailability, so monitoring of drug levels is required.
Product advantages
- 3PLUS1® Multilevel Calibrator Set available
- Tri-level controls available
- Tailor-made internal standard
This Chromsystems kit allows for reliable chromatographic determination of itraconazole, its active metabolite hydroxy-itraconazole, and posaconazole and voriconazole in only one isocratic HPLC run with fluorescence detection. Two very simple and efficient protein precipitation steps are used to separate interfering components and to obtain stable eluates. A tailor-made internal standard ensures reliable and precise quantification of the analytes.
These analytes - together with four further antimycotics - can also be determined using LC-MS/MS with the TDM Parameter Set Antimycotic Drugs from the MassTox® TDM Series A.
Number of Tests | 100 | ||||||||||||||||||||||||||||||||||||||||
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Limit of quantification | Itraconazole/OH-Itraconazole: 0.03 mg/l |
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Linearity | Itraconazole/OH-Itraconazole: up to 10 mg/l |
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Recovery | 101–105 % | ||||||||||||||||||||||||||||||||||||||||
Intraassay | CV = 1.1– 4.4 % |
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Interassay | CV = 2.4– 5.2 % |
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Analysis Time | Itraconazole/OH-Itraconazole: 13 min; Posaconazole/Voriconazole: 8 min | ||||||||||||||||||||||||||||||||||||||||
Sample Preparation |
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Sample Stability | The prepared samples can be kept in sealed glass vials for 1 week at +18 °C to +30 °C and for 2 weeks at +2 °C to +8 °C. Do not store refrigerated! | ||||||||||||||||||||||||||||||||||||||||
Specimen | Serum/Plasma | ||||||||||||||||||||||||||||||||||||||||
Pre-analytic Treatment | Storage life is up to 2 weeks at ambient temperature, at +2 °C to +8 °C up to 4 weeks. For longer storage (max. 3 months) keep samples below -18 °C. | ||||||||||||||||||||||||||||||||||||||||
Injection Volume | 20 µl (10 to 30 µl) | ||||||||||||||||||||||||||||||||||||||||
Flow rate | 1.2 ml/min | ||||||||||||||||||||||||||||||||||||||||
Column temperature | ambient (~ 25 °C) | ||||||||||||||||||||||||||||||||||||||||
Wavelength | EX 261 nm EM 366 nm |
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Gradient | isocratic | ||||||||||||||||||||||||||||||||||||||||
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. | ||||||||||||||||||||||||||||||||||||||||
Method of Analysis | HPLC | ||||||||||||||||||||||||||||||||||||||||
Parameter | Hydroxyitraconazole, Itraconazole, Posaconazole, Voriconazole | ||||||||||||||||||||||||||||||||||||||||
Complete Specification |
HPLC-parametersFor the HPLC analysis of itraconazole, posaconazole and voriconazole any isocratic HPLC system with fluorescence detector is suitable.
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Additional Info | Any isocratic HPLC system with fluorescence detector is suitable. |
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Mobile Phase, 10 x 1000 mlOrder no.: 27022Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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Itraconazole/Posaconazole/Voriconazole Plasma Calibration StandardOrder no.: 27034Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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Internal StandardOrder no.: 27044Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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Precipitation Reagent 1Order no.: 27055Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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Precipitation Reagent 2Order no.: 27066Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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Itraconazole/Posaconazole/Voriconazole Plasma Calibration StandardOrder no.: 27034Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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3PLUS1® Multilevel Plasma Calibrator Set Itraconazole/Posaconazole/VoriconazoleOrder no.: 27029Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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HPLC Column Itraconazole, Posaconazole and Voriconazole in Serum/PlasmaOrder no.: 27110Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC
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Precolumn Cartridge 4/10Order no.: 18027Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC