Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC

Order No.: 43000, for 100 tests
Parameters:
Hippuric Acid, m-Methylhippuric Acid, Mandelic Acid, o-Methylhippuric Acid, p-Methylhippuric Acid, Phenylglyoxylic Acid

Only 10 µl sample volume
Internal standard included
Simple sample preparation

Hippuric Acid

m-Methylhippuric Acid

Mandelic Acid

o-Methylhippuric Acid

p-Methylhippuric Acid

Phenylglyoxylic Acid

Clinical relevance

Hippuric acid, o-, m-, p-methylhippuric acid, mandelic acid and phenylglyoxylic acid are the main metabolites of the organic solvents: toluene, xylene, and styrene. These toxic compounds are widely used throughout various industries, making the determination of their concentrations relevant to occupational health in a number of different professional fields. Due to their high levels of volatility, they primarily enter the body through inhalation and are rapidly absorbed through the lungs. For occupational health monitoring of people who are exposed to these compounds at work, measurement of the metabolites in urine is more reliable than determining the concentrations of the toxins in the blood, due to their very fast metabolism rates.

For any hazardous substance or its metabolite, a BTV (Biological Tolerance Value) is defined as a threshold value for maximum absorption in biological material. Solvent-related harm to human health can be prevented by performing regular monitoring of urine.

 

Product advantages

  • Only 10 µl sample volume
  • Optimised internal standard
  • Straightforward sample preparation

 

This Chromsystems kit provides rapid and reliable determination of the main metabolites of toluene, xylene, and styrene in a single run. A 10 µl urine sample is all that is required, which is then analysed using an isocratic HPLC system with UV detection following a simple and fast preparation step. The inclusion of a tailor-made internal standard reduces analytical variations and guarantees high precision and reliability of results.

More Information
Number of Tests 100
Limit of quantification 4–11 mg/l
Linearity Hippuric acid: up to 18000 mg/l Methylhippuric acid: up to 7000 mg/l Mandelic acid: up to 4000 mg/l Phenylglyoxylic acid: up to 1650 mg/l
Recovery 102–109 %
Intraassay CV = 0.7–1.1 %

Interassay CV = 0.9–4.6 %
Analysis Time approx. 20 min
BEI values

Hippuric acid: 1500 mg/l; Methylhippuric acid: 2000 mg/l; Mandelic acid: 400 mg/l; Phenylglyoxylic acid: 100 mg/l

Sample Preparation
  • Pipette 1000 µl Internal Standard into a labelled reaction vial.
  • Add 10 µl urine,mix briefly (vortex).
  • Centrifuge 5 min at 9000 x g.
  • Inject 20 µl supernatant into the HPLC system.
Sample Stability The prepared samples are stable for 24 hours at room temperature, at +2 to +8 °C up to 5 days and below -18 °C up to 14 days.
Specimen Urine, collected after end of shift
Pre-analytic Treatment

Patient sample must be kept cool for transport. Storage life is up to 5 days at +2 to +8 °C and up to 3 months at -18 °C. Avoid repeated freeze/thaw cycles.

 

Injection Volume 20 µl
Flow rate 1.0 ml/min
Column temperature ambient (~ 25 °C)
Wavelength 207 nm
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Hippuric Acid, m-Methylhippuric Acid, Mandelic Acid, o-Methylhippuric Acid, p-Methylhippuric Acid, Phenylglyoxylic Acid
Complete Specification
Linearity/Limit of quantification:
  Limit of quantification: Linearity:
Hippuric acid: 15 up to 18000 mg/l
Methylhippuric acid: 15 up to 7000 mg/l
Mandelic acid: 15 up to 4000 mg/l
Phenylglyoxylic acid: 15 up to 1650 mg/l
Intra-assay:  
Inter-assay:  
Recovery:  
Run time: aproxx. 20 min  
Specimens: urine  
Stability of specimens:  
BEI values
Hippuric acid: 1500 mg/l  
Methylhippuric acid: 2000 mg/l  
Mandelic acid: 400 mg/l  
Phenylglyoxylic acid: 100 mg/l  
 

HPLC parameters

For the Chromsystems HPLC analysis of occupational medicine parameters in urine any isocratic HPLC system with UV detector is suitable.

Injection volume: 20 µl
Flow rate: 1.0 ml/min
Wavelength: 207 nm
Column temperature: ambient (~25 °C)
Additional Info

Any isocratic HPLC system with UV detector is suitable.

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    Mobile Phase
    Order no.: 43001
    Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC
    Details
  2. 43001 HPLC mobile phase hippuric acid methylhippuric acid mandelic acid phenylglyoxylic acid urine
    Mobile Phase, 10 x 1000 ml
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    Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC
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    Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC
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  4. 43004 HPLC internal standard occupational medicine urine
    Internal Standard
    Order no.: 43004
    Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC
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  5. 3006 reaction vials
    Reaction Vials
    Order no.: 3006
    Details
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  2. 43100 HPLC column occupational medicine urine
    HPLC Column Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine
    Order no.: 43100
    Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC
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  3. PEEK-encased Prefilter, 5 µm
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  4. Precolumn Cartridge Holder 4/10
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  5. Precolumn Cartridge 4/10
    Precolumn Cartridge 4/10
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    Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC
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