Hippuric Acid, Methylhippuric Acids, Mandelic Acid and Phenylglyoxylic Acid in Urine - HPLC

Order No.: 43000, for 100 tests
Parameters:
Hippuric Acid, m-Methylhippuric Acid, Mandelic Acid, o-Methylhippuric Acid, p-Methylhippuric Acid, Phenylglyoxylic Acid

Only 10 µl sample volume
Internal standard included
Simple sample preparation

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Hippuric Acid

m-Methylhippuric Acid

Mandelic Acid

o-Methylhippuric Acid

p-Methylhippuric Acid

Phenylglyoxylic Acid

Clinical relevance

Hippuric acid, o-, m-, p-methylhippuric acid, mandelic acid and phenylglyoxylic acid are the main metabolites of the organic solvents: toluene, xylene, and styrene. These toxic compounds are widely used throughout various industries, making the determination of their concentrations relevant to occupational health in a number of different professional fields. Due to their high levels of volatility, they primarily enter the body through inhalation and are rapidly absorbed through the lungs. For occupational health monitoring of people who are exposed to these compounds at work, measurement of the metabolites in urine is more reliable than determining the concentrations of the toxins in the blood, due to their very fast metabolism rates.

For any hazardous substance or its metabolite, a BTV (Biological Tolerance Value) is defined as a threshold value for maximum absorption in biological material. Solvent-related harm to human health can be prevented by performing regular monitoring of urine.

 

Product advantages

  • Only 10 µl sample volume
  • Optimised internal standard
  • Straightforward sample preparation

 

This Chromsystems assay provides rapid and reliable determination of the main metabolites of toluene, xylene, and styrene in a single run. A 10 µl urine sample is all that is required, which is then analysed using an isocratic HPLC system with UV detection following a simple and fast preparation step. The inclusion of a tailor-made internal standard reduces analytical variations and guarantees high precision and reliability of results.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 4–11 mg/l
Upper Limit of Quantification Hippuric acid: up to 18000 mg/l Methylhippuric acid: up to 7000 mg/l Mandelic acid: up to 4000 mg/l Phenylglyoxylic acid: up to 1650 mg/l
Intraassay CV = 0.7–1.1 %

Interassay CV = 0.9–4.6 %
Recovery 102–109 %
BEI values

Hippuric acid: 1500 mg/l; Methylhippuric acid: 2000 mg/l; Mandelic acid: 400 mg/l; Phenylglyoxylic acid: 100 mg/l

Specimen Urine, collected after end of shift
Sample Preparation
  • Pipette 1000 µl Internal Standard into a labelled reaction vial.
  • Add 10 µl urine,mix briefly (vortex).
  • Centrifuge 5 min at 9000 x g.
  • Inject 20 µl supernatant into the HPLC system.
Run Time approx. 20 min
Injection Volume 20 µl
Flow Rate 1.0 ml/min
Column Temperature ambient (~ 25 °C)
Wavelengths 207 nm
Additional Info

Any isocratic HPLC system with UV detector is suitable.

Parameters Hippuric Acid, m-Methylhippuric Acid, Mandelic Acid, o-Methylhippuric Acid, p-Methylhippuric Acid, Phenylglyoxylic Acid
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Hippuric Acid

m-Methylhippuric Acid

Mandelic Acid

o-Methylhippuric Acid

p-Methylhippuric Acid

Phenylglyoxylic Acid

Clinical relevance

Hippuric acid, o-, m-, p-methylhippuric acid, mandelic acid and phenylglyoxylic acid are the main metabolites of the organic solvents: toluene, xylene, and styrene. These toxic compounds are widely used throughout various industries, making the determination of their concentrations relevant to occupational health in a number of different professional fields. Due to their high levels of volatility, they primarily enter the body through inhalation and are rapidly absorbed through the lungs. For occupational health monitoring of people who are exposed to these compounds at work, measurement of the metabolites in urine is more reliable than determining the concentrations of the toxins in the blood, due to their very fast metabolism rates.

For any hazardous substance or its metabolite, a BTV (Biological Tolerance Value) is defined as a threshold value for maximum absorption in biological material. Solvent-related harm to human health can be prevented by performing regular monitoring of urine.

 

Product advantages

  • Only 10 µl sample volume
  • Optimised internal standard
  • Straightforward sample preparation

 

This Chromsystems assay provides rapid and reliable determination of the main metabolites of toluene, xylene, and styrene in a single run. A 10 µl urine sample is all that is required, which is then analysed using an isocratic HPLC system with UV detection following a simple and fast preparation step. The inclusion of a tailor-made internal standard reduces analytical variations and guarantees high precision and reliability of results.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 4–11 mg/l
Upper Limit of Quantification Hippuric acid: up to 18000 mg/l Methylhippuric acid: up to 7000 mg/l Mandelic acid: up to 4000 mg/l Phenylglyoxylic acid: up to 1650 mg/l
Intraassay CV = 0.7–1.1 %

Interassay CV = 0.9–4.6 %
Recovery 102–109 %
BEI values

Hippuric acid: 1500 mg/l; Methylhippuric acid: 2000 mg/l; Mandelic acid: 400 mg/l; Phenylglyoxylic acid: 100 mg/l

Specimen Urine, collected after end of shift
Sample Preparation
  • Pipette 1000 µl Internal Standard into a labelled reaction vial.
  • Add 10 µl urine,mix briefly (vortex).
  • Centrifuge 5 min at 9000 x g.
  • Inject 20 µl supernatant into the HPLC system.
Run Time approx. 20 min
Injection Volume 20 µl
Flow Rate 1.0 ml/min
Column Temperature ambient (~ 25 °C)
Wavelengths 207 nm
Additional Info

Any isocratic HPLC system with UV detector is suitable.

Parameters Hippuric Acid, m-Methylhippuric Acid, Mandelic Acid, o-Methylhippuric Acid, p-Methylhippuric Acid, Phenylglyoxylic Acid
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.