Hemoglobin Variants - HPLC

Order No.: 15330, for 1000 tests
HbA, HbA1c, HbA2, HbC, HbD, HbE, HbF, HbS

All important hemoglobin variants within 9 min.
Improved sample throughput and efficiency









Clinical relevance

The haemoglobin molecule is responsible for transporting oxygen inside of the human organism, and consists of four globin polypeptide chains and one haem molecule. In humans, the globin polypeptide chains are further subdivided in α, β, γ and δ chains. Human haemoglobin usually consists of two α chains and two additional globin chains (β, γ or δ). In adults, the major component of haemoglobin is HbA (ααββ). Its glycated forms are referred to as HbA1a, HbA1b and HbA1c. HbA2 consists of two α chains and two δ chains. Foetal haemoglobin (HbF) consists of two α chains and two β chains.

Genetic disorders of haemoglobin production are called thalassaemias – they most commonly affect the β chain. This leads to β-thalassaemia. It is especially common in populations in the Mediterranean region, the Middle East and East Asia, but it also occurs in Middle Europe and North America as a result of demographic change.


Product advantages

  • Measurement of all key haemoglobin variants in just 9 minutes
  • Clear identifiable peaks and reliable values


By making use of the high separating capacities of the analytical column, the Chromsystems kit for the analysis of haemoglobin variants can determine the most common variants HbA1c, HbA2, HbC, HbD, HbE, HbF and HbS within 9 minutes. A column material specially designed for this specific separation process also allows a stable separation of HbA2 from HbD and HbE even with aged columns. This helps to eliminate false quantifications and qualifications.

More Information
Number of Tests 1000
Limit of quantification

HbA2: 2 %
HbF: 1 %


HbA2: up to at least 75 %
HbF: up to at least 70 %

Recovery HbA2: 100 %; HbF: 99 %

CV < 8 %


CV < 4 %

Analysis Time 9 min
Sample Preparation
  • Dilute 5 to 8 µl whole blood with 1 ml Hemolysis Reagent, mix well (vortex).
  • Inject 10 to 20 µl of the hemolysis mixture into the HPLC system.

Sample Stability Prepared samples can be stored at ambient temperature for about 24 h, at +2 to +8 °C up to 36 h, below -18 °C up to 3 months.
Specimen Whole blood
Pre-analytic Treatment The Patient samples are stable at room temperature for 3 days, at +2 to +8 °C up to 1 week and below -18 °C up to 6 months. Once thawed, samples should not be re-frozen.
Injection Volume 10-20 µl
Flow rate 1.5 ml/min
Column temperature ambient temperature (~ 25 °C)
Wavelength 415 nm
Gradient binary
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter HbA, HbA1c, HbA2, HbC, HbD, HbE, HbF, HbS
Complete Specification
Linearity: up to at least 75 % (HbA2)
up to at least 70 % (HbF)
Limit of quantification: 2 % (HbA2)
1 % (HbF)
Intra-assay: < 8 %
Inter-assay: < 4 %
Recovery: 100 % (HbA2)
99.2 % (HbF)
Run time: approx. 9 min

HPLC parameters

For the analysis of hemoglobin variants any HPLC gradient system with UV/VIS detector is suitable.

Injection volume: 10-20 µl
Flow rate: 1.5 ml/min
Wavelength: 415 nm
Column temperature: ambient (approx. 25 °C)
Additional Info Any HPLC gradient system with UV/VIS detector is suitable.
Pressure Maximum pressure: 140 bar
  1. Buffer A - 2.1 l
    Buffer A - 2.1 l
    Order no.: 15331
    Hemoglobin Variants - HPLC
  2. Buffer A - 5 l
    Buffer A - 5 l
    Order no.: 15331/C
    Hemoglobin Variants - HPLC
  3. Buffer B - 3 l
    Buffer B - 3 l
    Order no.: 15332
    Hemoglobin Variants - HPLC
  4. Buffer B - 5 l
    Buffer B - 5 l
    Order no.: 15332/C
    Hemoglobin Variants - HPLC
  5. Hemolysis Reagent
    Hemolysis Reagent
    Order no.: 15334
    Hemoglobin Variants - HPLC
  6. Wash Buffer
    Wash Buffer
    Order no.: 15335
    Hemoglobin Variants - HPLC
  7. HPLC Column Hemoglobin Variants
    HPLC Column Hemoglobin Variants
    Order no.: 15390
    Hemoglobin Variants - HPLC
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
  2. Reaction Vials
    Reaction Vials
    Order no.: 15007/B
  3. PEEK-encased Prefilter, 5 µm
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