CDT in Serum with Fast Elution - HPLC
Short analysis time
Detects established variants
Exact separation of asialo-, disialo- and trisialotransferrin < 6 min
Manual or automated sample preparation with 96 well plate
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation
Excessive alcohol consumption leads to specific changes in the glycosylation pattern of transferrin. This results in the increasing formation of asialo- and disialotransferrin in the serum, which are referred to as Carbohydrate-Deficient-Transferrins (CDT). CDT and in particular disialotransferrin is regarded as the most specific and most sensitive laboratory marker of chronic alcohol abuse. A daily intake of more than 60 g ethanol (about 0.75 l wine) over a longer period elevates CDT values significantly. These return to normal after alcohol abstinence. This makes CDT an excellent parameter for controlling withdrawal treatment, forensic assessment or in occupational medicine.
- Specific marker for chronic alcohol abuse
- Run time of less than 10 min
- Detection of genetic variants
- Traceable to the IFCC reference measurement procedure
This Chromsystems assay is designed for the specific and fast determination of CDT in serum with a ternary HPLC gradient system. During sample preparation, lipoproteins are removed by precipitation with simultaneous iron saturation. The chromatography enables a very good separation of asialo-Tf, disialo-Tf and trisialo-Tf in less than 6 minutes. The transferrins are separated using two mobile phases, thereafter the column is cleaned with a third mobile phase.
This method is traceable to the IFCC reference measurement procedure (IFCC-RMP) and certified accordingly. It can therefore be used to determine CDTIFCC (CDT as disialotransferrin).
|Method of Analysis||HPLC|
|Number of Tests||500|
|Limit of quantification||
approx. 0.5 % Disialotransferrin
|Linearity||up to at least 12 % Disialotransferrin|
CV = 5.7 %
CV = 6.3 %
|Analysis Time||9.5 min|
Specimens should be kept cool for transport. Storage life is up to 1 week at +2 to +8 °C. For longer storage, deep-freeze below –18°C.
* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 µl each per sample)
|Injection Volume||100-150 µl|
|Flow rate||2.5 ml/min|
|Column temperature||+30 °C|
|Additional Info||For the Chromsystems HPLC analysis of CDT FE in serum (Fast Elution) a ternary HPLC gradient system with UV detection is necessary.|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Parameter||Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin|