CDT in Serum with Fast Elution - HPLC

Order No.: 54330/500, for 500 tests
Parameters:
Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Short analysis time
Detects established variants
Exact separation of asialo-, disialo- and trisialotransferrin < 6 min
Manual or automated sample preparation with 96 well plate

Clinical relevance


Excessive alcohol consumption leads to specific changes in the glycosylation pattern of transferrin. This results in the increasing formation of asialo- and disialotransferrin in the serum, which are referred to as Carbohydrate-Deficient-Transferrins (CDT). CDT and in particular disialotransferrin is regarded as the most specific and most sensitive laboratory marker of chronic alcohol abuse. A daily intake of more than 60 g ethanol (about 0.75 l wine) over a longer period elevates CDT values significantly. These return to normal after alcohol abstinence. This makes CDT an excellent parameter for controlling withdrawal treatment, forensic assessment or in occupational medicine.

 

Product advantages

  • Specific marker for chronic alcohol abuse
  • Run time of less than 10 min
  • Detection of genetic variants
  • Traceable to the IFCC reference measurement procedure

This Chromsystems assay is designed for the specific and fast determination of CDT in serum with a ternary HPLC gradient system. During sample preparation, lipoproteins are removed by precipitation with simultaneous iron saturation. The chromatography enables a very good separation of asialo-Tf, disialo-Tf and trisialo-Tf in less than 6 minutes. The transferrins are separated using two mobile phases, thereafter the column is cleaned with a third mobile phase.


This method is traceable to the IFCC reference measurement procedure (IFCC-RMP) and certified accordingly. It can therefore be used to determine CDTIFCC (CDT as disialotransferrin).

More Information
Method of Analysis HPLC
Number of Tests 500
Limit of quantification

approx. 0.5 % Disialotransferrin

Linearity up to at least 12 % Disialotransferrin
Intraassay

CV = 5.7 %

Interassay

CV = 6.3 %

Analysis Time 9.5 min
Specimen Serum
Pre-analytic Treatment

Specimens should be kept cool for transport. Storage life is up to 1 week at +2 to +8 °C. For longer storage, deep-freeze below –18°C.

Sample Preparation
  • Place 200 µl serum/control into a reaction vial.
  • Add 100 µl reagent mix*, mix for 60 s (vortex).
  • Incubate for 30 min at +2 bis +8 °C.
  • Centrifuge for 10 min at 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 150 µl into the HPLC system.

* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 µl each per sample)

Injection Volume 100-150 µl
Flow rate 2.5 ml/min
Column temperature +30 °C
Wavelength 460 nm
Additional Info For the Chromsystems HPLC analysis of CDT FE in serum (Fast Elution) a ternary HPLC gradient system with UV detection is necessary.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin
  1. Mobile Phase A
    Mobile Phase A
    Order no.: 54331

    CDT (FE) in Serum with Fast Elution - HPLC

    Details
  2. Mobile Phase B
    Mobile Phase B
    Order no.: 54332

    CDT (FE) in Serum with Fast Elution - HPLC

    Details
  3. Mobile Phase C
    Mobile Phase C
    Order no.: 54333

    CDT (FE) in Serum with Fast Elution - HPLC

    Details
  4. 54025 HPLC neutralisation buffer CDT serum
    Neutralisation Buffer
    Order no.: 54025

    CDT in Serum - HPLC

    Details
  5. 54026 HPLC stabilisation buffer CDT serum
    Stabilisation Buffer
    Order no.: 54026
    CDT in Serum - HPLC
    Details
  6. 54027 HPLC precipitation reagent 1 CDT serum
    Precipitation Reagent 1
    Order no.: 54027
    CDT in Serum - HPLC
    Details
  7. 54028 HPLC precipitation reagent 2 CDT serum
    Precipitation Reagent 2
    Order no.: 54028

    CDT in Serum - HPLC

    Details
  8. 3006 reaction vials
    Reaction Vials
    Order no.: 3006
    Details
  1. 0168 0169 CDT serum controls
    CDT Serum Controls
    Order no.: 0168/0169
    Details
  1. 54100 HPLC column CDT serum
    HPLC Column CDT in Serum
    Order no.: 54100

    CDT in Serum - HPLC

    Details
  1. 54100 HPLC column CDT serum
    HPLC Column CDT in Serum
    Order no.: 54100

    CDT in Serum - HPLC

    Details
  2. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  3. PEEK-encased Prefilter, 5 µm
    Details
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