Catecholamines in Urine - HPLC
Easy sample preparation
Easy pH adjustment through Neutralisation Buffer with integrated indicator
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
Adrenaline (Epinephrine)
Dopamine
Noradrenaline (Norepinephrine)
Clinical relevance
The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.
The determination of catecholamines has clinical significance in the diagnosis of pheochromocytomas and other tumours affecting the nervous system. These diseases result in the production of surplus catecholamines, which are then excreted in the urine. 24-hour urine collection is the preferred method for tumour screening. Furthermore, the concentrations of adrenaline and noradrenaline are important indicators for cardiac insufficiency, cardiac diseases and arteriosclerosis.
This Chromsystems assay allows the quantitative determination of adrenaline, noradrenaline and dopamine in human urine samples via HPLC (high performance liquid chromatography) with electrochemical detection.
It is intended as a monitoring test for patients with suspected catecholamine-secreting tumour.
Product advantages
• Easy sample preparation
• Easy pH adjustment through Neutralisation Buffer with integrated indicator
Assay characteristics
Prior to chromatographic separation, the analytes are separated from the urine matrix by ion exchange. Sample preparation requires pH-adjustment of the diluted urine, transfer onto the Sample Clean Up Column and two washing steps.
The assays 2020/COMBI and 6000/COMBI are available as alternative methods that allow the combined determination of catecholamines and metanephrine with one column.
There is also a method for the HPLC analysis of catecholamines in plasma available (order no. 5000).
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | Adrenaline/Epinephrine: 1.6 ng/l – 1800 ng/l The limit of quantification depends on the condition of the working electrode. |
| Specimen | 24 h urine (containing 10 ml 25 % HCl) or spontaneous urine (refer the data to urine creatinine). |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | < 15 min |
| Injection Volume | 20 µl |
| Flow Rate | 0.8-1.3 ml/min |
| Column Temperature | ambient (~25 °C) |
| Potential | approx. 400-500 mV |
| Additional Info | For the HPLC analysis of catecholamines any isocratic HPLC system with electrochemical detector is suitable. |
| Parameters | Adrenaline (Epinephrine), Dopamine, Noradrenaline (Norepinephrine) |
Adrenaline (Epinephrine)
Dopamine
Noradrenaline (Norepinephrine)
Clinical relevance
The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.
The determination of catecholamines has clinical significance in the diagnosis of pheochromocytomas and other tumours affecting the nervous system. These diseases result in the production of surplus catecholamines, which are then excreted in the urine. 24-hour urine collection is the preferred method for tumour screening. Furthermore, the concentrations of adrenaline and noradrenaline are important indicators for cardiac insufficiency, cardiac diseases and arteriosclerosis.
This Chromsystems assay allows the quantitative determination of adrenaline, noradrenaline and dopamine in human urine samples via HPLC (high performance liquid chromatography) with electrochemical detection.
It is intended as a monitoring test for patients with suspected catecholamine-secreting tumour.
Product advantages
• Easy sample preparation
• Easy pH adjustment through Neutralisation Buffer with integrated indicator
Assay characteristics
Prior to chromatographic separation, the analytes are separated from the urine matrix by ion exchange. Sample preparation requires pH-adjustment of the diluted urine, transfer onto the Sample Clean Up Column and two washing steps.
The assays 2020/COMBI and 6000/COMBI are available as alternative methods that allow the combined determination of catecholamines and metanephrine with one column.
There is also a method for the HPLC analysis of catecholamines in plasma available (order no. 5000).
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | Adrenaline/Epinephrine: 1.6 ng/l – 1800 ng/l The limit of quantification depends on the condition of the working electrode. |
| Specimen | 24 h urine (containing 10 ml 25 % HCl) or spontaneous urine (refer the data to urine creatinine). |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | < 15 min |
| Injection Volume | 20 µl |
| Flow Rate | 0.8-1.3 ml/min |
| Column Temperature | ambient (~25 °C) |
| Potential | approx. 400-500 mV |
| Additional Info | For the HPLC analysis of catecholamines any isocratic HPLC system with electrochemical detector is suitable. |
| Parameters | Adrenaline (Epinephrine), Dopamine, Noradrenaline (Norepinephrine) |