β-Carotene in Serum/Plasma - HPLC
Fast and easy sample preparation
Low costs per test
High sample stability
Further analytes detectable: Cryptoxanthin, Lycopene, α-Carotene, cis-β-Carotene
β-carotene (provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. Like glutathione or vitamin C, it possesses antioxidative properties. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products.
β-carotene can only be processed by the body when absorbed together with fat. Any additional high-dosed preventive therapy should be used with caution to prevent a possible overdose. Additional use of high dose β-carotene over several years combined with nicotine and alcohol consumption may even increase the risk of lung or bowel cancer. For this reason, medications that contain β-carotene are labelled with a warning for smokers. Furthermore, drugs with high concentrations of β-carotene are no longer allowed to be prescribed to smokers either.
- Simple, fast and effective sample preparation
- High sample stability
- Low costs per test
This reagent kit is designed for easy and reliable determination of β-carotene in serum/plasma. The sample clean up procedure combines a fast precipitation and extraction step. The resulting extract is soluble in the mobile phase, so no evaporation is necessary. The chromatographic determination is run on an isocratic HPLC system with UV/VIS detection. A special RP-chromatography ensures a reliable separation of α-, cis-β-, and all-trans-β-carotene as well as some further carotenoids in less than 10 minutes. The analytes are quantified by the inclusion of an internal standard, which is a non-naturally occurring carotenoid-derivative, so that only a single detection wavelength is required. The high stability of the processed samples allows for easier performance of large sample batches.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||5 ng/ml|
|Linearity||up to 3000 ng/ml|
CV ≤ 2.0 %
CV ≤ 3.1 %
|Analysis Time||approx. 10 min|
|Pre-analytic Treatment||The patient samples are stable at +2 to +8 °C up to 5 days, light-protected in a closed vial. For longer storage (up to 2 months) keep samples below -18 °C. Specimens should be kept frozen and light-protected for transport.|
|Sample Stability||The prepared samples are stable for 5 days at room temperature, at +2 to 8 °C up to 1 week (in glass vials and light protected). For longer storage (up to 4 weeks) keep them below -18 °C.|
|Injection Volume||50 µl|
|Flow rate||1.5-1.8 ml/min|
|Column temperature||ambient (~25 °C)|
Any isocratic HPLC system with pump, injector and UV detector is suitable.
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|