Antiepileptic Drugs in Serum/Plasma - automated HPLC

Order No.: 23000/F, for 100 tests
Parameters:
10-OH-Carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Lamotrigine, Oxcarbamazepine (Trileptal®), Phenobarbital, Phenytoin, Primidone, Sultiame, Zonisamide

Sample preparation is completely automated with Gilson® ASPEC™ XL

Carbamazepine

10-OH-Carbamazepine

Carbamazepine-10,11-epoxide

Oxcarbamazepine (Trileptal®)

Ethosuximide

Lamotrigine

Phenobarbital

Phenytoin

Primidone

Sultiame

Zonisamide

Chromsystems kit for automated HPLC analysis of Antiepileptic Drugs in Serum/Plasma, Fast Elution, Application for Gilson® ASPEC™XL.

 

Kit content
Mobile Phase
Calibration Standard, lyophilised
Internal Standard
Equilibration Buffer
Wash Buffer
Elution Buffer
Sample Clean Up Columns with DEC-Caps

More Information
Number of Tests 100
Specimen Serum/Plasma
Information Using of a precolumn is necessary.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter 10-OH-Carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Lamotrigine, Oxcarbamazepine (Trileptal®), Phenobarbital, Phenytoin, Primidone, Sultiame, Zonisamide
Complete Specification

Linearity/Limit of quantification: The method is linear from the designated limit of quantification up to the upper limit.

Analyte Limit of quantification Linear range
(mg/l) up to (mg/l)
Sultiame 0.7 50
Ethosuximide 4.5 250
Zonisamide 0.3 100
Primidone 0.2 40
Lamotrigine 0.2 30
10-OH-Carbamazepine 2.0
40
Phenobarbital 0.6 150
Carbamazepin-10,11-Epoxide 0.2 40
Oxcarbamazepine 0.6 50
Phenytoin 0.3 50
Carbamazepine 0.3 50
Recovery: 84-103 %
Intra-assay: Vk: < 3 %
Inter-assay: Vk: < 6 %
Run time: Fast Elution: < 5 min
High Resolution: < 22 min

HPLC parameters

Isocratic HPLC system with UV detector

Injection volume: 20 µl
Flow rate:: Fast Elution: 2.0 ml/min
High Resolution: 1.2 ml/min
Wavelength: 204 nm
Column temperature: ambient (~25 °C)

A precolumn has to be used.

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