5-HIAA in Urine - HPLC
Easy sample preparation
No exact pH-adjustment necessary
The alternative to immunoassay methods
Compatible with the VMA/HVA/5-HIAA method
5-Hydroxyindoleacetic Acid (5-HIAA)
Clinical relevance
A number of diseases are associated with pathological changes in serotonin metabolism. In carcinoid tumour, a malignant hyperplasia of the enterochromaffin cells of the gastrointestinal tract, there is a marked increase in serotonin release into blood plasma. This tissue hormone is metabolised enzymatically in the liver by monoamine oxidase (MAO) and aldehyde dehydrogenase (ADH) to 5-hydroxyindole-3-acetic acid (5-HIAA) and subsequently excreted via the kidney. Endocrine-active carcinoid thus results in an increased concentration of 5-HIAA in the urine. The quantitative determination of 5-HIAA in urine is therefore used for the diagnosis of the carcinoid tumour and for therapy monitoring.
Product advantages
- Easy sample preparation
- No exact pH adjustment required
- The alternative to immunoassay methods
- Compatible with the Chromsystems VMA/HVA/5-HIAA method
This kit for the determination of 5-HIAA in urine provides a rapid and reliable sample preparation procedure with only one precipitation step. The kit contains all the components in a ready-to-use form for sample preparation and HPLC analysis. A carefully optimised chromatographic separation is part of the Chromsystems complete solution. A selected HPLC column and a mobile phase optimised specifically for this separation permit a reliable quantification.
Urine samples prepared with this kit can also be analysed with column and mobile phase of the VMA/HVA/5-HIAA Chromsystems kit.
Kit content for 100 analyses:
Mobile Phase, 1000 ml
Internal Standard, 10 ml
Urine Calibration Standard (lyoph.), 2 x 5 ml
Precipitation Reagent, 75 ml
Reaction vials, 100 pcs.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Limit of quantification | 0.5 mg/l |
| Linearity | up to at least 25 mg/l |
| Recovery | 101 % |
| Intraassay | CV = 2.6-6.9 % |
| Interassay | CV = 4.0-4.1 % |
| Analysis Time | ~ 9 min |
| Specimen | 24 h-urines collected with 10 ml glacial acetic acid. |
| Pre-analytic Treatment | 24h-urine collected with 10 ml glacial acetic acid, pH 4 - 6. Stored in the dark at +2 to +8 °C patient samples are stable up to 3 days. For longer storage periods patient samples should be frozen below -18 °C. |
| Sample Preparation | Place a 200 μl aliquot of the acidified urine sample in a test tube or reaction vial. |
| Sample Stability | Stored at +2 to +8 °C the samples are stable up to 3 days. |
| Injection Volume | 10-20 µl |
| Flow rate | 0.8 - 1.3 ml/min |
| Column temperature | ambient (~25 °C) |
| Additional Info | For the HPLC analysis 5-HIAA in urine any isocratic HPLC system with electrochemical detector is suitable. |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Parameter | 5-Hydroxyindoleacetic Acid (5-HIAA) |
| Potential | +620 to +680 mV |
