Parameter Set Tricyclic Antidepressants TCA 2 - LC-MS/MS

Order No.: 92920
Parameters:
Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine

Encompasses 7 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Clomipramine

Norclomipramine

Maprotiline

Normaprotiline

Protriptyline

Trimipramine

Nortrimipramine

Clinical relevance

Tricyclic antidepressants (TCAs) are used to treat psychological conditions such as anxiety, pain syndromes, and phobias, and produce a positive mood enhancing effect. Their pharmacological effect is based on increasing the concentration of dopamine, noradrenaline and serotonin in the synaptic cleft by inhibiting the degradation or reuptake of these neurotransmitters. TCAs are highly lipophilic and are absorbed rapidly from the gastrointestinal tract after oral administration. Even before they reach their site of action in the brain, a fraction is already metabolised in the liver (first-pass effect). Regular determination of the plasma levels can minimise the risk of side effects resulting from overdose. Furthermore, patient compliance can be checked by this procedure to help ensure optimal therapeutic success.

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 1.5 – 10 µg/l
Upper Limit of Quantification at least twice that of the therapeutic range
Intraassay CV = 1.7 – 4.3 %
Interassay CV = 3.8 – 6.3 %
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix
  • Add 800 μl Internal Standard mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time 3.5 min
Injection Volume 0.2 – 50 µl
Gradient Isocratic (35 % Mobile Phase 1; 65 % Mobile Phase 2)
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Clomipramine

Norclomipramine

Maprotiline

Normaprotiline

Protriptyline

Trimipramine

Nortrimipramine

Clinical relevance

Tricyclic antidepressants (TCAs) are used to treat psychological conditions such as anxiety, pain syndromes, and phobias, and produce a positive mood enhancing effect. Their pharmacological effect is based on increasing the concentration of dopamine, noradrenaline and serotonin in the synaptic cleft by inhibiting the degradation or reuptake of these neurotransmitters. TCAs are highly lipophilic and are absorbed rapidly from the gastrointestinal tract after oral administration. Even before they reach their site of action in the brain, a fraction is already metabolised in the liver (first-pass effect). Regular determination of the plasma levels can minimise the risk of side effects resulting from overdose. Furthermore, patient compliance can be checked by this procedure to help ensure optimal therapeutic success.

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 1.5 – 10 µg/l
Upper Limit of Quantification at least twice that of the therapeutic range
Intraassay CV = 1.7 – 4.3 %
Interassay CV = 3.8 – 6.3 %
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix
  • Add 800 μl Internal Standard mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time 3.5 min
Injection Volume 0.2 – 50 µl
Gradient Isocratic (35 % Mobile Phase 1; 65 % Mobile Phase 2)
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.