Parameter Set Antiepileptic Drugs XT2 All-in-One Method - LC-MS/MS

Order No.: 92921-XT2
Parameters:
10-OH-Carbamazepine, Brivaracetam, Carbamazepine, Carbamazepine-10,11-epoxide, Cenobamate, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam, N-Desmethylmesuximide, Oxcarbamazepine, Perampanel, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

Updated parameter menu including cenobamate
Each analyte covered by its own internal standard
Determination of all analytes in a single run
3PLUS1® Multilevel Calibrator Set and MassCheck® controls
Part of the continuously extended MassTox® TDM Series A

Validated according to IVDR (=> Declaration of Conformity)

Brivaracetam

Carbamazepine

Carbamazepine-10,11-epoxide

10-OH-Carbamazepine

Cenobamate

N-Desmethylmesuximide

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam

Oxcarbazepine

Perampanel

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

Stiripentol

Sultiame

Theophylline

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamide

Clinical relevance

Epileptic seizures are the result of synchronous discharges of neuron groups in the brain that can lead to sudden and involuntary stereotypical behavioural or sensory disorders. Numerous types of seizures have been described, each of which requires specialised therapy. The probability of them occurring depends on a number of factors. In addition to genetic predisposition, also exogenous factors are relevant, such as accident, thrombosis, tumours or meningitis. Therapy with anticonvulsive medication (antiepileptics) leads to a reduction in seizures and sometimes even a complete elimination of seizures in most treated patients. The precondition for the antiepileptics to have a therapeutic effect is usually patient compliance, which means regular use of the medication. Thus, monitoring of the blood levels is essential.
Theophylline, which can also be determined with this assay, is primarily used to treat asthma and chronic obstructive pulmonary disease (COPD). An overdose of this substance can lead to seizures. 

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

 

Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation

Brivaracetam: 0.11 mg/L – 9.00 mg/L
Cenobamate: 0.24 mg/L – 84.2 mg/L
Lamotrigine: 0.43 mg/L – 35.0 mg/L
Levetiracetam: 0.17 mg/L – 180 mg/L
Perampanel: 0.011 mg/L – 3.75 mg/L

For the values of all analytes please refer to the instruction manual.

Different systems might show different performance data.

Specimen Serum/Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Add 800 µL Internal Standard Mix to 12 mL Precipitation Reagent (= Internal Standard Solution).
  • Pipette 50 µL sample/calibrator/MassCheck® control into a 1.5 mL reaction vial.
  • Add 25 µL Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µL of the prepared Internal Standard Solution, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (according to instruction manual) and inject ≤ 10 µL into LC-MS/MS system.
Run Time 4.9 min
Injection Volume ≤ 10 µL
Gradient binary
Additional Info Autosampler cooling recommended
Ionisation ESI positive and negative
MS/MS Mode MRM
Additional Info

This method further includes 28 internal standards, which are not shown in the chromatogram for clarity.

To avoid ESI switching for ethosuximide, phenobarbital and valproic acid, the analysis can be performed separately in ESI negative mode.

Parameters 10-OH-Carbamazepine, Brivaracetam, Carbamazepine, Carbamazepine-10,11-epoxide, Cenobamate, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam, N-Desmethylmesuximide, Oxcarbamazepine, Perampanel, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Brivaracetam

Carbamazepine

Carbamazepine-10,11-epoxide

10-OH-Carbamazepine

Cenobamate

N-Desmethylmesuximide

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam

Oxcarbazepine

Perampanel

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

Stiripentol

Sultiame

Theophylline

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamide

Clinical relevance

Epileptic seizures are the result of synchronous discharges of neuron groups in the brain that can lead to sudden and involuntary stereotypical behavioural or sensory disorders. Numerous types of seizures have been described, each of which requires specialised therapy. The probability of them occurring depends on a number of factors. In addition to genetic predisposition, also exogenous factors are relevant, such as accident, thrombosis, tumours or meningitis. Therapy with anticonvulsive medication (antiepileptics) leads to a reduction in seizures and sometimes even a complete elimination of seizures in most treated patients. The precondition for the antiepileptics to have a therapeutic effect is usually patient compliance, which means regular use of the medication. Thus, monitoring of the blood levels is essential.
Theophylline, which can also be determined with this assay, is primarily used to treat asthma and chronic obstructive pulmonary disease (COPD). An overdose of this substance can lead to seizures. 

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

 

Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation

Brivaracetam: 0.11 mg/L – 9.00 mg/L
Cenobamate: 0.24 mg/L – 84.2 mg/L
Lamotrigine: 0.43 mg/L – 35.0 mg/L
Levetiracetam: 0.17 mg/L – 180 mg/L
Perampanel: 0.011 mg/L – 3.75 mg/L

For the values of all analytes please refer to the instruction manual.

Different systems might show different performance data.

Specimen Serum/Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Add 800 µL Internal Standard Mix to 12 mL Precipitation Reagent (= Internal Standard Solution).
  • Pipette 50 µL sample/calibrator/MassCheck® control into a 1.5 mL reaction vial.
  • Add 25 µL Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µL of the prepared Internal Standard Solution, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (according to instruction manual) and inject ≤ 10 µL into LC-MS/MS system.
Run Time 4.9 min
Injection Volume ≤ 10 µL
Gradient binary
Additional Info Autosampler cooling recommended
Ionisation ESI positive and negative
MS/MS Mode MRM
Additional Info

This method further includes 28 internal standards, which are not shown in the chromatogram for clarity.

To avoid ESI switching for ethosuximide, phenobarbital and valproic acid, the analysis can be performed separately in ESI negative mode.

Parameters 10-OH-Carbamazepine, Brivaracetam, Carbamazepine, Carbamazepine-10,11-epoxide, Cenobamate, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam, N-Desmethylmesuximide, Oxcarbamazepine, Perampanel, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.