Catecholamines in Plasma - HPLC

Order No.: 5000, for 200 tests
Parameters:
Adrenaline (Epinephrine), Dopamine, Noradrenaline (Norepinephrine)

Easy sample preparation
Low analysis costs

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Adrenaline (Epinephrine)

Dopamine

Noradrenaline (Norepinephrine)

Clinical relevance

The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.

The determination of catecholamines has clinical significance in the diagnosis of pheochromocytomas and other tumours affecting the nervous system. These diseases result in the production of surplus catecholamines, which are then excreted in the urine. 24-hour urine collection as the preferred method for tumour screening. The concentrations of adrenaline and noradrenaline are important indicators for cardiac insufficiency, cardiac diseases and arteriosclerosis.

This assay allows the quantitative determination of adrenaline, noradrenaline and dopamine in human plasma samples via high performance liquid chromatography (HPLC) with electrochemical detection.
It is intended as a monitoring test for patients with suspected catecholamine-secreting tumour.

 

Product advantages

• Easy sample preparation
• Low analysis costs

 

Assay characteristics

Before HPLC analysis, the analytes are extracted from the plasma matrix by adsorption on alumina. Sample preparation is simple, because pH-adjustment of the plasma samples is not necessary. Moreover, sample preparation requires only washing steps with Wash Buffer.

For the HPLC analysis of catecholamines in urine, there is an additional method available (order no. 6000).

Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.

More Information
Method of Analysis HPLC
Number of Tests 200
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

Adrenaline/Epinephrine: 18 ng/l – 2250 ng/l
Noradrenaline/Norepinephrine: 43 ng/l – 4500 ng/l
Dopamine: 81 ng/l – 3500 ng/l

The limit of quantification depends on the condition of the working electrode.

Specimen Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Add 500 µl Extraction Buffer to a sample clean up cartridge, shake briefly.
  • Apply 1 to 1.5 ml plasma and 50 µl Internal Standard.
  • Mix for 10 min by inverting.
  • Remove the bottom plug and remove supernatant by vacuum or centrifugation, discard effluent.
  • 3 times: apply 1 ml Wash Buffer, shake briefly, centrifuge (1 min at 700 x g, after the last wash step 2 min at 2800 x g), discard effluent.
  • Mount the plastic tube to the outlet of the cartridge, add 120 µl Elution Buffer, shake briefly, incubate for 5 min. Vortex 30 s.
  • Centrifuge for 1 min at 700 x g into the attached plastic tube. Collect eluat and mix well.
  • Inject 20-50 µl into the HPLC system.
Run Time < 20 min
Injection Volume 20-50 µl
Flow Rate 0.8-1.3 ml/min
Column Temperature ambient (~25 °C)
Potential 400 - 500 mV
Additional Info

For the HPLC analysis of catecholamines in plasma any isocratic HPLC system with electrochemical detector is suitable.

Parameters Adrenaline (Epinephrine), Dopamine, Noradrenaline (Norepinephrine)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Adrenaline (Epinephrine)

Dopamine

Noradrenaline (Norepinephrine)

Clinical relevance

The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.

The determination of catecholamines has clinical significance in the diagnosis of pheochromocytomas and other tumours affecting the nervous system. These diseases result in the production of surplus catecholamines, which are then excreted in the urine. 24-hour urine collection as the preferred method for tumour screening. The concentrations of adrenaline and noradrenaline are important indicators for cardiac insufficiency, cardiac diseases and arteriosclerosis.

This assay allows the quantitative determination of adrenaline, noradrenaline and dopamine in human plasma samples via high performance liquid chromatography (HPLC) with electrochemical detection.
It is intended as a monitoring test for patients with suspected catecholamine-secreting tumour.

 

Product advantages

• Easy sample preparation
• Low analysis costs

 

Assay characteristics

Before HPLC analysis, the analytes are extracted from the plasma matrix by adsorption on alumina. Sample preparation is simple, because pH-adjustment of the plasma samples is not necessary. Moreover, sample preparation requires only washing steps with Wash Buffer.

For the HPLC analysis of catecholamines in urine, there is an additional method available (order no. 6000).

Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.

More Information
Method of Analysis HPLC
Number of Tests 200
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

Adrenaline/Epinephrine: 18 ng/l – 2250 ng/l
Noradrenaline/Norepinephrine: 43 ng/l – 4500 ng/l
Dopamine: 81 ng/l – 3500 ng/l

The limit of quantification depends on the condition of the working electrode.

Specimen Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Add 500 µl Extraction Buffer to a sample clean up cartridge, shake briefly.
  • Apply 1 to 1.5 ml plasma and 50 µl Internal Standard.
  • Mix for 10 min by inverting.
  • Remove the bottom plug and remove supernatant by vacuum or centrifugation, discard effluent.
  • 3 times: apply 1 ml Wash Buffer, shake briefly, centrifuge (1 min at 700 x g, after the last wash step 2 min at 2800 x g), discard effluent.
  • Mount the plastic tube to the outlet of the cartridge, add 120 µl Elution Buffer, shake briefly, incubate for 5 min. Vortex 30 s.
  • Centrifuge for 1 min at 700 x g into the attached plastic tube. Collect eluat and mix well.
  • Inject 20-50 µl into the HPLC system.
Run Time < 20 min
Injection Volume 20-50 µl
Flow Rate 0.8-1.3 ml/min
Column Temperature ambient (~25 °C)
Potential 400 - 500 mV
Additional Info

For the HPLC analysis of catecholamines in plasma any isocratic HPLC system with electrochemical detector is suitable.

Parameters Adrenaline (Epinephrine), Dopamine, Noradrenaline (Norepinephrine)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.