Catecholamines in Plasma - HPLC

Order No.: 5000, for 200 tests
Parameters:
Adrenaline (Epinephrine), Dopamine, Noradrenaline

Easy and safe handling
All components are ready-for-use
High sensitivity
Low analysis costs

Adrenaline (Epinephrine)

Dopamine

Noradrenaline

Clinical relevance

The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.

The determination of catecholamines has clinical significance in the diagnosis of pheochromocytomas and other tumours affecting the nervous system. These diseases result in the production of surplus catecholamines, which are then excreted in the urine. 24-hour urine collection is the preferred method for tumour screening. The plasma concentration is determined to localise the tumour or to perform pharmacological function tests. Furthermore, the concentrations of adrenaline and noradrenaline are important indicators for cardiac insufficiency, cardiac diseases and arteriosclerosis.

 

Product advantages

  • Simple and safe handling
  • Low detection limits
  • Low analysis costs
  • Ready-to-use components

 

Our established kit allows the standard routine analysis of adrenaline, noradrenaline and dopamine in plasma with an isocratic HPLC system and electrochemical detector. A very specific sample preparation using solid phase extraction guarantees simple and safe handling of the samples as well as reproducible results.

For the HPLC analysis of catecholamines in urine, there is another method (# 6000) available.

 

Kit content

Mobile Phase, 1000 ml
Extraction Buffer, 100 ml
Catecholamines Calibration Standard, 10 ml
Internal Standard, 10 ml
Wash Buffer, 300 ml
Elution Buffer, 25 ml
Sample Clean Up Columns, 50 pcs.

More Information
Number of Tests 200
Limit of quantification

Adrenaline, Noradrenaline: 18-43 ng/l
Dopamine: 81 ng/l

Linearity

2250–4000 ng/l

Recovery > 70 % (dopamine > 40 %)
Intraassay

CV < 3.8 %

Interassay

CV < 6 % (dopamine ~13 %)

Analysis Time < 20 min
Sample Preparation
  • Add 500 µl Extraction Buffer to a sample clean up cartridge, shake briefly.
  • Apply 1 to 1.5 ml plasma and 50 µl Internal Standard.
  • Mix for 10 min by inverting.
  • Remove the bottom plug and remove supernatant by vacuum or centrifugation, discard effluent.
  • 3 times: apply 1 ml Wash Buffer, shake briefly, centrifuge (1 min at 2000 rpm, after the last wash step 2 min at 4000 rpm), discard effluent.
  • Mount the plastic tube to the outlet of the cartridge, add 120 µl Elution Buffer, shake briefly, incubate for 5 min. Vortex 30 s.
  • Centrifuge for 1 min at 2000 rpm into the attached plastic tube. Collect eluat and mix well.
  • Inject 20-50 µl into the HPLC system.
Sample Stability At ambient temperature up to 2 hours; below -18 °C up to 9 months
Specimen Plasma
Pre-analytic Treatment

Sample drawing

  • Blood samples should be drawn from the recumbent patient.
  • 20 µl stabilisation solutuion (900 mg EGTA, 700 mg glutathione in 0,5 M NaOH ad 8 ml, pH 7-7.5 with H2O ad 10 ml) is required per ml blood.
  • Mix, centrifuge.
Injection Volume 20-50 µl
Flow rate 0.8-1.3 ml/min
Column temperature ambient (~25 °C)
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Adrenaline (Epinephrine), Dopamine, Noradrenaline
Additional Info

For the HPLC analysis of catecholamines in plasma any isocratic HPLC system with electrochemical detector is suitable.

Potential 400 - 500 mV
  1. 5001 HPLC mobile phase catecholamines plasma urine
    Mobile Phase
    Order no.: 5001
    Catecholamines in Plasma or in Urine - HPLC
    Details
  2. 5001 HPLC mobile phase catecholamines plasma urine
    Mobile Phase, 10 x 1000 ml
    Order no.: 5002
    Catecholamines in Plasma or Urine - HPLC
    Details
  3. 5011 HPLC extraction buffer catecholamines plasma
    Extraction Buffer
    Order no.: 5011
    Catecholamines in Plasma - HPLC
    Details
  4. 5003 HPLC plasma calibration standard catecholamines
    Calibration Standard
    Order no.: 5003
    Catecholamines in Plasma - HPLC
    Details
  5. 5004 HPLC internal standard catecholamines plasma
    Internal Standard
    Order no.: 5004
    Catecholamines in Plasma - HPLC
    Details
  6. 5005 HPLC wash buffer catecholamines plasma
    Wash Buffer
    Order no.: 5005
    Catecholamines in Plasma - HPLC
    Details
  7. 5006 HPLC elution buffer catecholamines plasma
    Elution Buffer
    Order no.: 5006
    Catecholamines in Plasma - HPLC
    Details
  8. 5007 HPLC sample clean up columns catecholamines plasma
    Sample Clean Up Columns
    Order no.: 5007
    Catecholamines in Plasma - HPLC
    Details
  1. 5009 HPLC Plasma Calibration Standard Catecholamines Plasma
    Plasma Calibration Standard
    Order no.: 5009
    Catecholamines in Plasma - HPLC
    Details
  2. 5003 HPLC plasma calibration standard catecholamines
    Calibration Standard
    Order no.: 5003
    Catecholamines in Plasma - HPLC
    Details
  1. 0010 0020 HPLC endocrine plasma controls
    Endocrine Plasma Controls
    Order no.: 0010/0020
    Details
  1. 5007-Vi HPLC plastic vials for sample clean up columns
    Plastic Vials for Sample Clean Up Columns
    Order no.: 5007/Vi
    Catecholamines in Plasma - HPLC
    Details
  2. 5100 HPLC column catecholamines plasma
    HPLC column
    Order no.: 5100
    Catecholamines in Plasma
    Details
As a customer please login or register to gain full access.