MassChrom® Amino Acid Analysis in Urine with 96 Deep Well Plates - LC-MS/MS
Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
Creatinine normalisation within the same run
Fast sample preparation without derivatisation
Shorter run times than HPLC methods
Excellent separation of all isobaric analytes
Each analyte with its own internal standard
One chromatographic platform for plasma/serum and urine
Assay validated according to IVDR (=> Declaration of Conformity)
|
Acetyltyrosine Adenosylhomocysteine Alanine β-Alanine α-Aminoadipic acid α-Aminobutyric acid β-Aminoisobutyric acid γ-Aminobutyric acid Anserine Arginine Argininosuccinic acid Asparagine Aspartic acid Carnosine |
Citrulline Homocitrulline Cystathionine Cystine Cysteine S-sulfate Homocystine Ethanolamine Phosphoethanolamine Glutamic acid Glutamine Glycine Histidine 1-Methylhistidine 3-Methylhistidine |
Kynurenine Hydroxykynurenine Leucine Allo-Isoleucine Isoleucine Lysine Hydroxylysine Methionine Ornithine Oxoproline / Pyroglutamic acid Phenylacetylglutamine Phenylalanine Phosphoserine Pipecolic acid |
Proline 4-Hydroxyproline Saccharopine Sarcosine Serine Taurine Threonine Tryptophan Tyrosine Valine Creatinine * |
* With this assay the creatinine level in urine can be determined within the same run!
Clinical relevance
This assay enables the quantitative determination of 53 amino acids and metabolites in urine including the parameter creatinine, so that the results can be directly related to the creatinine value without further analysis. It is intended to be used for patients with suspected metabolic diseases in whom the urinary amino acid and metabolic product levels are of clinical importance, specifically as an aid to
- diagnosis of cystinuria
- diagnosis of lysinuric protein intolerance
- differential diagnosis of Hartnup disorder versus other aminoacidurias (cystinuria and generalized aminoaciduria)
- monitoring of treatment efficacy in previously diagnosed cystinuria patients.
Product advantages
- Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
- Creatinine normalisation within the same run
- Fast sample preparation without derivatisation
- Shorter run times than HPLC methods
- Excellent separation of all isobaric analytes
- Each analyte with its own internal standard
- One chromatographic platform for plasma/serum and urine
Assay characteristics
A differentiated amino acid profile can be created in less than 20 minutes. Sample preparation consists of a fast and easy urine dilution. The method does not require derivatisation, making it robust and time-saving. Isobaric compounds are separated chromatographically. Each of the 53 analytes is safeguarded by its own internal standard compensating variations in recovery, matrix effects and ionisation effectiveness. The 3PLUS1® Multilevel Calibrator Set and MassCheck® controls are produced from synthetic urine to ensure that their composition approximates a patient sample as closely as possible. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Download Brochure
You need all the facts at a glance? Then you are welcome to download our brochure. After you have filled out the following form, the respective link to download your document will be sent to the e-mail address provided.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 3 x 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | ULOQ for each analyte: up to double concentration of highest calibrator For the exact values (LLOQ and ULOQ) of all analytes please refer to the instruction manual. |
| Specimen | Urine |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | < 20 min |
| Injection Volume | up to 6 µl (mass spectrometer dependent) |
| Flow Rate | 0.3–1.8 ml/min |
| Column Temperature | + 25°C |
| Gradient | binary gradient, flow gradient |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | α-Aminoadipic Acid, α-Aminobutyric Acid, β-Alanine, β-Aminoisobutyric Acid, γ-Aminobutyric Acid, 1-Methylhistidine, 3-Methylhistidine, 4-Hydroxyproline, Acetyltyrosine, Adenosylhomocysteine, Alanine, Allo-Isoleucine, Anserine, Arginine, Argininosuccinic Acid, Asparagine, Aspartic Acid, Carnosine, Citrulline, Creatinine, Cystathionine, Cysteine S-sulfate, Cystine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocitrulline, Homocystine, Hydroxykynurenine, Hydroxylysine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Oxoproline / Pyroglutamic acid, Phenylacetylglutamine, Phenylalanine, Phosphoserine, Pipecolic Acid, Proline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Valine |
75222 MassChrom® Amino Acid Analysis in Urine with 96 Deep Well Plates
For 3 x 96 tests
| Order No. | Kit components (Quantities in kit) |
| 75001 | Mobile Phase A, 950 ml (4x) |
| 75002 | Mobile Phase B, 950 ml (1x) |
| 75009 | Rinsing Solution, 500 ml (1x) |
| 75246 | Internal Standard Set, consisting of: Internal Standard Mix, 3 x 5.0 ml (lyoph.), Reconstitution Buffer, 3 x 5.5 ml |
| 75205 | Dilution Buffer, 90 ml (2x) |
| 75156 | 96 Deep Well Plates, 4 pcs. (1x) |
| 75058 | Collection Plates, 4 pcs. (1x) |
| 75060 | Pierceable Heat Seals for 96 Well Plates, 5 pcs. (1x) |
| Order No. | Required components (not included in the kit) |
| 75100 | Analytical Column, equilibrated, with test chromatogram, 1 pc. |
| 75228 | 3PLUS1® Multilevel Urine Calibrator Set Amino Acid Analysis, 4 x 0.5 ml (lyoph.) |
| 0474 | MassCheck® Amino Acid Analysis Urine Control, Level I, 5 x 0.5 ml (lyoph.) |
| 0475 | MassCheck® Amino Acid Analysis Urine Control, Level II, 5 x 0.5 ml (lyoph.) |
| 0476 | MassCheck® Amino Acid Analysis Urine Control, Level III, 5 x 0.5 ml (lyoph.) |
| 75015 | Tuning Mix 1, Analytes and Internal Standards, 1 ml |
| 75016 | Tuning Mix 2, Analytes and Internal Standards, 1 ml |
| 75017 | Tuning Mix 3, Analytes and Internal Standards, 1 ml |
| 75018 | Tuning Mix 4, Analytes and Internal Standards, 1 ml |
| 75019 | Tuning Mix 5, Analytes and Internal Standards, 1 ml |
| 75220 | Tuning Mix 6, Analytes and Internal Standards, 1 ml |
| 75212 | Adjustment Reagent, for high amino acid concentrations, 30 ml |
| Order No. | General lab equipment (non-CE/IVD products) |
| 15010 | PEEK Prefilter Housing, 1 pc. |
| 15013 | PEEK Prefilter, 2 µm, 5 pcs. |
| 15080 | Back Pressure Regulator Valve, 1 pc. |
| 42740 | Chromsystems Heat Sealer, 1 pc. |
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-
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Rinsing SolutionOrder no.: 75009MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS -
MassChrom® Amino Acid Analysis - Urine SetOrder no.: 75211/DWPMassChrom® Amino Acid Analysis in Urine with 96 Deep Well Plates
Consisting of reagents and lab equipment
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS -
3PLUS1® Multilevel Urine Calibrator Set Amino Acid AnalysisOrder no.: 75228MassChrom® Amino Acid Analysis in Urine – LC-MS/MS -
MassCheck® Amino Acid Analysis Urine ControlsOrder no.: 0474/0475/0476MassChrom® Amino Acid Analysis in Urine - LC-MS/MS -
Tuning Mix 1 Amino Acid AnalysisOrder no.: 75015MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS -
Tuning Mix 2 Amino Acid AnalysisOrder no.: 75016MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS -
Tuning Mix 3 Amino Acid AnalysisOrder no.: 75017MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS -
Tuning Mix 4 Amino Acid AnalysisOrder no.: 75018MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS -
Tuning Mix 5 Amino Acid AnalysisOrder no.: 75019MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS -
Tuning Mix 6 Amino Acid Analysis in UrineOrder no.: 75220MassChrom® Amino Acid Analysis in Urine – LC-MS/MS
-
3PLUS1® Multilevel Urine Calibrator Set Amino Acid AnalysisOrder no.: 75228MassChrom® Amino Acid Analysis in Urine – LC-MS/MS
-
MassCheck® Amino Acid Analysis Urine ControlsOrder no.: 0474/0475/0476MassChrom® Amino Acid Analysis in Urine - LC-MS/MS
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Chromsystems Heat SealerOrder no.: 42740Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint
|
Acetyltyrosine Adenosylhomocysteine Alanine β-Alanine α-Aminoadipic acid α-Aminobutyric acid β-Aminoisobutyric acid γ-Aminobutyric acid Anserine Arginine Argininosuccinic acid Asparagine Aspartic acid Carnosine |
Citrulline Homocitrulline Cystathionine Cystine Cysteine S-sulfate Homocystine Ethanolamine Phosphoethanolamine Glutamic acid Glutamine Glycine Histidine 1-Methylhistidine 3-Methylhistidine |
Kynurenine Hydroxykynurenine Leucine Allo-Isoleucine Isoleucine Lysine Hydroxylysine Methionine Ornithine Oxoproline / Pyroglutamic acid Phenylacetylglutamine Phenylalanine Phosphoserine Pipecolic acid |
Proline 4-Hydroxyproline Saccharopine Sarcosine Serine Taurine Threonine Tryptophan Tyrosine Valine Creatinine * |
* With this assay the creatinine level in urine can be determined within the same run!
Clinical relevance
This assay enables the quantitative determination of 53 amino acids and metabolites in urine including the parameter creatinine, so that the results can be directly related to the creatinine value without further analysis. It is intended to be used for patients with suspected metabolic diseases in whom the urinary amino acid and metabolic product levels are of clinical importance, specifically as an aid to
- diagnosis of cystinuria
- diagnosis of lysinuric protein intolerance
- differential diagnosis of Hartnup disorder versus other aminoacidurias (cystinuria and generalized aminoaciduria)
- monitoring of treatment efficacy in previously diagnosed cystinuria patients.
Product advantages
- Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
- Creatinine normalisation within the same run
- Fast sample preparation without derivatisation
- Shorter run times than HPLC methods
- Excellent separation of all isobaric analytes
- Each analyte with its own internal standard
- One chromatographic platform for plasma/serum and urine
Assay characteristics
A differentiated amino acid profile can be created in less than 20 minutes. Sample preparation consists of a fast and easy urine dilution. The method does not require derivatisation, making it robust and time-saving. Isobaric compounds are separated chromatographically. Each of the 53 analytes is safeguarded by its own internal standard compensating variations in recovery, matrix effects and ionisation effectiveness. The 3PLUS1® Multilevel Calibrator Set and MassCheck® controls are produced from synthetic urine to ensure that their composition approximates a patient sample as closely as possible. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Download Brochure
You need all the facts at a glance? Then you are welcome to download our brochure. After you have filled out the following form, the respective link to download your document will be sent to the e-mail address provided.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 3 x 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | ULOQ for each analyte: up to double concentration of highest calibrator For the exact values (LLOQ and ULOQ) of all analytes please refer to the instruction manual. |
| Specimen | Urine |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | < 20 min |
| Injection Volume | up to 6 µl (mass spectrometer dependent) |
| Flow Rate | 0.3–1.8 ml/min |
| Column Temperature | + 25°C |
| Gradient | binary gradient, flow gradient |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | α-Aminoadipic Acid, α-Aminobutyric Acid, β-Alanine, β-Aminoisobutyric Acid, γ-Aminobutyric Acid, 1-Methylhistidine, 3-Methylhistidine, 4-Hydroxyproline, Acetyltyrosine, Adenosylhomocysteine, Alanine, Allo-Isoleucine, Anserine, Arginine, Argininosuccinic Acid, Asparagine, Aspartic Acid, Carnosine, Citrulline, Creatinine, Cystathionine, Cysteine S-sulfate, Cystine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocitrulline, Homocystine, Hydroxykynurenine, Hydroxylysine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Oxoproline / Pyroglutamic acid, Phenylacetylglutamine, Phenylalanine, Phosphoserine, Pipecolic Acid, Proline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Valine |
75222 MassChrom® Amino Acid Analysis in Urine with 96 Deep Well Plates
For 3 x 96 tests
| Order No. | Kit components (Quantities in kit) |
| 75001 | Mobile Phase A, 950 ml (4x) |
| 75002 | Mobile Phase B, 950 ml (1x) |
| 75009 | Rinsing Solution, 500 ml (1x) |
| 75246 | Internal Standard Set, consisting of: Internal Standard Mix, 3 x 5.0 ml (lyoph.), Reconstitution Buffer, 3 x 5.5 ml |
| 75205 | Dilution Buffer, 90 ml (2x) |
| 75156 | 96 Deep Well Plates, 4 pcs. (1x) |
| 75058 | Collection Plates, 4 pcs. (1x) |
| 75060 | Pierceable Heat Seals for 96 Well Plates, 5 pcs. (1x) |
| Order No. | Required components (not included in the kit) |
| 75100 | Analytical Column, equilibrated, with test chromatogram, 1 pc. |
| 75228 | 3PLUS1® Multilevel Urine Calibrator Set Amino Acid Analysis, 4 x 0.5 ml (lyoph.) |
| 0474 | MassCheck® Amino Acid Analysis Urine Control, Level I, 5 x 0.5 ml (lyoph.) |
| 0475 | MassCheck® Amino Acid Analysis Urine Control, Level II, 5 x 0.5 ml (lyoph.) |
| 0476 | MassCheck® Amino Acid Analysis Urine Control, Level III, 5 x 0.5 ml (lyoph.) |
| 75015 | Tuning Mix 1, Analytes and Internal Standards, 1 ml |
| 75016 | Tuning Mix 2, Analytes and Internal Standards, 1 ml |
| 75017 | Tuning Mix 3, Analytes and Internal Standards, 1 ml |
| 75018 | Tuning Mix 4, Analytes and Internal Standards, 1 ml |
| 75019 | Tuning Mix 5, Analytes and Internal Standards, 1 ml |
| 75220 | Tuning Mix 6, Analytes and Internal Standards, 1 ml |
| 75212 | Adjustment Reagent, for high amino acid concentrations, 30 ml |
| Order No. | General lab equipment (non-CE/IVD products) |
| 15010 | PEEK Prefilter Housing, 1 pc. |
| 15013 | PEEK Prefilter, 2 µm, 5 pcs. |
| 15080 | Back Pressure Regulator Valve, 1 pc. |
| 42740 | Chromsystems Heat Sealer, 1 pc. |
-
-
-
Rinsing SolutionOrder no.: 75009MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
MassChrom® Amino Acid Analysis - Urine SetOrder no.: 75211/DWP
MassChrom® Amino Acid Analysis in Urine with 96 Deep Well Plates
Consisting of reagents and lab equipment
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
3PLUS1® Multilevel Urine Calibrator Set Amino Acid AnalysisOrder no.: 75228MassChrom® Amino Acid Analysis in Urine – LC-MS/MS
-
MassCheck® Amino Acid Analysis Urine ControlsOrder no.: 0474/0475/0476MassChrom® Amino Acid Analysis in Urine - LC-MS/MS
-
Tuning Mix 1 Amino Acid AnalysisOrder no.: 75015MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Tuning Mix 2 Amino Acid AnalysisOrder no.: 75016MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Tuning Mix 3 Amino Acid AnalysisOrder no.: 75017MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Tuning Mix 4 Amino Acid AnalysisOrder no.: 75018MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Tuning Mix 5 Amino Acid AnalysisOrder no.: 75019MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Tuning Mix 6 Amino Acid Analysis in UrineOrder no.: 75220MassChrom® Amino Acid Analysis in Urine – LC-MS/MS
-
3PLUS1® Multilevel Urine Calibrator Set Amino Acid AnalysisOrder no.: 75228MassChrom® Amino Acid Analysis in Urine – LC-MS/MS
-
MassCheck® Amino Acid Analysis Urine ControlsOrder no.: 0474/0475/0476MassChrom® Amino Acid Analysis in Urine - LC-MS/MS
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Chromsystems Heat SealerOrder no.: 42740
Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint