Sample Prep Set Catecholamines, free Metanephrines, Serotonin with Sample Clean Up Columns

Order No.: 80600/C, for 96 tests

Parameters:

3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin

7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls

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Chromatogram

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

Parameters

3-Methoxytyramine

Adrenaline (Epinephrine)

Noradrenaline

Dopamine

Metanephrine

Normetanephrine

Serotonin

Description

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With Sample Clean Up Columns, for manual sample prep procedure

Clinical relevance

This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS. One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours.

Product advantages

7 analytes in one single run
Fast and efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results

This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

There is also a method using 96 SPE well plates (80600/96 & 80600/480) as well as the corresponding fully automated workflow solution available for laboratories with a higher sample throughput.

Technical Data

More Information
Number of Tests	96
Limit of quantification	1.0 - 13.5 μg/l
Linearity	1600 - 5000 µg/l
Intraassay	CV = 1.5 - 8.1 %
Interassay	CV = 3.1 - 10.7 %
Analysis Time	4 min
Sample Preparation	Pipette 200 µl of the sample/calibrator/*Mass*Check^® control into each Sample Clean Up Column. Add 50 µl Internal Standard Mix and 600 µl Neutralisation Buffer. Check for correct colour. Centrifuge 5 min at 150 x g, discard effluent. Add 0.9 ml Wash Buffer 1 and centrifuge 5 min at 150 x g, discard effluent. Add 2 x 0.9 ml Wash Buffer 2 and centrifuge each time 1 min at 150 x g, discard effluent. Centrifuge 1 min at 2000 x g to dryness, discard effluent. Put Sample Clean Up Columns on new glass collection vials (e.g. glass autosampler vials). Add 500 µl Elution Buffer and centrifuge 2 min at 100 x g. Add 500 µl Dilution Buffer to each glass vial. Seal each glass vial. Shortly vortex each glass vial and transfer vials to autosampler. Inject up to 10 µl.
Sample Stability	The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.
Specimen	24 h urine or spontaneous urine
Pre-analytic Treatment	24 h urine collected in a suitable container with 10 ml of 10–25 % HCl. Spontaneous urine can be also analysed (see instruction manual). Patient specimens are stable for at least 7 days at +2 to +8 °C in amber coloured vials. For longer storage periods keep samples frozen below -18 °C.
Injection Volume	≤ 10 µl
Ionisation	ESI positive
MS/MS-Mode	MRM
Please note	The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis	LC-MS/MS
Parameter	3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin

Components

PP Screw-on caps, rubber/PTFE septa, 9 mm
Order no.: J0504

Details
Autosampler Vials, screw neck, amber glass, 1.5 ml
Order no.: J0601

Details
Internal Standard Mix
Order no.: 80604

MassChrom^® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Details
Neutralisation Buffer
Order no.: 80605

MassChrom^® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Details
Wash Buffer 1
Order no.: 80606

MassChrom^® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Details
Wash Buffer 2
Order no.: 80607

MassChrom^® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Details
Elution Buffer
Order no.: 80608

MassChrom^® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Details
Dilution Buffer
Order no.: 80609

MassChrom^® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Details
Sample Clean Up Columns Mass Chrom® Biogenic Amines/Metabolites in Urine
Order no.: 80055

MassChrom^® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Details