One Step Vitamins A and E in Serum/Plasma - UHPLC
High throughput UHPLC methode
One step sample preparation with pre-mixed reaction tubes
Fully automated workflow available
Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.
Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.
Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.
However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.
- High throughput UHPLC method
- One-step sample preparation with pre-mixed tubes
- Automated version available
The new Chromsystems UHPLC kit for the analysis of vitamins A and E in plasma/serum combines the reliability of the Chromsystems method with the throughput and speed of UHPLC technology. Sample preparation is reduced to a single pipetting step using pre-mixed reaction tubes, which contain all necessary reagents and the customized internal standard, so all that is left to do is add the sample. Separation of the analytes is carried out in less than 4 minutes. For large numbers of samples there is also a method using 96-well filter plates available for semi-automated or automated sample preparation with a pipetting robot.
Mobile Phase (for UHPLC), 2 x 500 ml
Pre-mixed Reaction Tubes, 10 x 100 pcs.
Serum Calibration Standard Vitamins A and E (lyoph.), 5 x 1 ml
UHPLC column, 1 pc.
UHPLC Precolumn cartridge, 3 x 1 pc.
|Number of Tests||1000|
|Limit of quantification||Vitamin A: 0.005 mg/l
Vitamin E: 0.100 mg/l
|Linearity||Vitamin A: up to 4.5 mg/l
Vitamin E: up to 38 mg/l
|Recovery||Vitamin A: 104 %; Vitamin E: 101 %|
Vitamin A: CV = 0.6-1.3 %
Vitamin A: CV = 2.9-3.1 %
|Analysis Time||3.5 min|
|Sample Stability||The prepared, lightprotected samples are stable at ambient temperature for at least 24 h and at +2 to +8°C up to 48 h. For longer storage (up to 3 weeks) keep samples below -18 °C.|
Time of sample drawing
Collection and storage of specimens
|Injection Volume||5 µl|
|Flow rate||0.6 ml/min|
|Column temperature||35 °C|
|Wavelength||325 nm, after 1.30 min switch to 295 nm|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Method of Analysis||UHPLC|
|Parameter||Vitamin A, Vitamin E|
For the analysis of vitamins A and E in serum/plasma, any major UHPLC system with isocratic pump, programmable UV detector and column oven is suitable.
|Pressure||approx. 400 bar|