One Step CDT in Serum with Fast Elution - HPLC

Order No.: 54930/500, for 500 tests
Parameters:
Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

With ready-to-use pre-mixed tubes
Only one pipetting step
Detects genetic variants

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation

Asialotransferrin

Disialotransferrin

Pentasialotransferrin

Tetrasialotransferrin

Trisialotransferrin

Clinical relevance

CDT (carbohydrate-deficient transferrin) is a lab marker showing the highest specificity for chronic alcohol abuse. CDT values increase significantly with the daily intake of more than 60 g ethanol (the equivalent of 0.75 l wine) per day over a period of two weeks. The values return to normal within two weeks in case of alcohol abstention. This makes CDT an excellent parameter for continuous monitoring in withdrawal programmes or for assessments in forensic or occupational medicine.

Unlike other biochemical parameters like γ-GT or MCV, liver diseases will not cause any false-positive results.

 

Product advantages

  • Very easy sample preparation with pre-mixed tubes
  • Only one pipetting step required
  • Detection of genetic variants

 

This Chromsystems assay allows for the fast and reliable measurement of CDT in serum using HPLC and UV detection. Sample preparation with premixed tubes is performed in a single pipetting step, making it fast and convenient.

The CDT is displayed as an area percentage of total transferrin, so it remains unaffected by fluctuations in the concentration of transferrin in serum. Optimised chromatographic separation of transferrin isoforms also allows for the reliable detection of known genetic variants.

A manual method with automated sample preparation is also available as an alternative for greater sample throughput.

More Information
Method of Analysis HPLC
Number of Tests 500
Limit of quantification approx. 0.5 % Disialotransferrin
Intraassay CV = 2.4 % (with 3.3 % Disialo)
Interassay CV = 3.2 % (with 3.3 % Disialo)
Analysis Time 10 min
Specimen Serum
Pre-analytic Treatment

Specimens
Serum (no anticoagulants), no EDTA or heparin plasma!

Stability of specimens
For longer transport send cooled at +2 to +8 °C, deep freezing is possible.

Sample Preparation
  • Add 100 µl serum to a Pre-mix Tube.
  • Vortex for 60 sec.
  • Incubate 30 min at +2 to +8°C.
  • Centrifuge 10 min at 9000 x g.
  • Inject 150–200 µl of the undiluted supernatant into the HPLC system.
Sample Stability 6 days at ambient temperature
Injection Volume 150-200 µl
Flow rate Detection: 1.5 ml/min, Rinsing: 2.5 ml/min
Column temperature Ambient (~25 °C)
Wavelength 460 nm
Additional Info For the Chromsystems HPLC analysis of CDT in serum with fast elution chromatography a ternary HPLC gradient system with UV detection is necessary.

Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin
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