Mycophenolic Acid in Plasma/Serum - HPLC

Order No.: 46000, for 100 tests
Parameters:
Mycophenolic Acid

Fast and easy sample preparation
Removes interfering substanzes for a precise analysis
Detection of physiologically active MPA

 

Mycophenolic Acid

Clinical relevance

Mycophenolic acid (MPA) is used as an immunosuppressant agent to prevent organ transplant rejection. It is also used to treat autoimmune disorders with chronic inflammation of the skin (psoriasis), eyes, and the digestive tract (Crohn's disease).

Currently available drugs contain mycophenolate mofetil or mycophenolate sodium, which are both completely metabolised into the active metabolite MPA. MPA is partially further metabolised into the inactive mycophenolate glucuronide (MPAG), which then can undergo deglucuronidation in the enterohepatic circulation system and is available again in the body as an active metabolite. Therapeutic monitoring of MPA is required to reach blood concentrations within the therapeutic window to achieve a favourable ratio between therapeutic effects and side effects for the patient.

 

Product advantages

  • Simple and fast sample preparation
  • No interferences

This Chromsystems kit is designed to determine the active metabolite mycophenolic acid in plasma or serum using HPLC. Sample preparation is performed by means of selective solid phase extraction, in which physiologically inactive MPAG is reliably and clearly separated. A stable internal standard ensures exact quantification. The HPLC column separates interfering substances, including the antibiotic daptomycin, to guarantee reliable and reproducible results.

Our TDM Parameter Set Mycophenolic Acid from the MassTox® TDM Series A is available as an alternative for the measurement of MPA and MPAG using LC-MS/MS.

More Information
Number of Tests 100
Limit of quantification 0.1 mg/l
Linearity up to 30 mg/l
Recovery 99 %
Intraassay CV ≤ 1.2 %
Interassay CV ≤ 3.3 %
Analysis Time 8–9 min
Sample Preparation
  • Centrifuge specimens before sample preparation (10 min at 2800 x g)!
  • Precondition the Sample Clean Up Columns with 1 ml Equilibration Buffer 1 and draw through by centrifugation (approx. 30–60 s at 190 x g) or suction.
  • Repeat with 1 ml Equilibration Buffer 2.
  • Mix 250 μl plasma/serum sample with 250 μl Internal Standard.
  • Apply complete sample to the Sample Clean Up Column and draw through by centrifugation (1 min at 190 x g) or suction; discard effluent.
  • Draw 2 x 1 ml Wash Buffer through Sample Clean Up Column by centrifugation (2 min at 1100 x g) or suction; discard effluent.
  • Elute the mycophenolic acid with 400 μl of the Elution Buffer.
  • Inject 20–40 μl eluate into the HPLC system.
Sample Stability The prepared samples are stable for 3 days at room temperature, at +2 to +8 °C up to 1 week. For longer storage keep samples below -18 °C.
Specimen plasma/serum
Pre-analytic Treatment Patient samples are stable at +2 to +8 °C for 4 weeks, below -18 °C up to 3 months. Keep samples cool for transport.
Injection Volume 20–40 µl
Flow rate 1 ml/min
Column temperature ambient (~ 25 °C)
Wavelength 215 nm
Gradient isocratic
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Mycophenolic Acid
Additional Info

Any isocratic HPLC system with UV detector is suitable.

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