Mycophenolic Acid in Plasma/Serum - HPLC
Fast and easy sample preparation
Removes interfering substanzes for a precise analysis
Detection of physiologically active MPA
Mycophenolic acid (MPA) is used as an immunosuppressant agent to prevent organ transplant rejection. It is also used to treat autoimmune disorders with chronic inflammation of the skin (psoriasis), eyes, and the digestive tract (Crohn's disease).
Currently available drugs contain mycophenolate mofetil or mycophenolate sodium, which are both completely metabolised into the active metabolite MPA. MPA is partially further metabolised into the inactive mycophenolate glucuronide (MPAG), which then can undergo deglucuronidation in the enterohepatic circulation system and is available again in the body as an active metabolite. Therapeutic monitoring of MPA is required to reach blood concentrations within the therapeutic window to achieve a favourable ratio between therapeutic effects and side effects for the patient.
- Simple and fast sample preparation
- No interferences
This Chromsystems kit is designed to determine the active metabolite mycophenolic acid in plasma or serum using HPLC. Sample preparation is performed by means of selective solid phase extraction, in which physiologically inactive MPAG is reliably and clearly separated. A stable internal standard ensures exact quantification. The HPLC column separates interfering substances, including the antibiotic daptomycin, to guarantee reliable and reproducible results.
Our TDM Parameter Set Mycophenolic Acid from the MassTox® TDM Series A is available as an alternative for the measurement of MPA and MPAG using LC-MS/MS.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||0.1 mg/l|
|Linearity||up to 30 mg/l|
|Intraassay||CV ≤ 1.2 %|
|Interassay||CV ≤ 3.3 %|
|Analysis Time||8–9 min|
|Pre-analytic Treatment||Patient samples are stable at +2 to +8 °C for 4 weeks, below -18 °C up to 3 months. Keep samples cool for transport.|
|Sample Stability||The prepared samples are stable for 3 days at room temperature, at +2 to +8 °C up to 1 week. For longer storage keep samples below -18 °C.|
|Injection Volume||20–40 µl|
|Flow rate||1 ml/min|
|Column temperature||ambient (~ 25 °C)|
Any isocratic HPLC system with UV detector is suitable.
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|