MassChrom® Methylmalonic Acid in Serum/Plasma and Urine - LC-MS/MS

Order No.: 64000, for 400 tests
Parameters:
Methylmalonic Acid (MMA)

Clean-up tubes for fast preparation of plasma and serum samples
Direct injection of urine samples after dilution
Chromatographic separation of succinic acid

Methylmalonic Acid (MMA)

Clinical relevance

Methylmalonic acid (MMA) is an early, sensitive biomarker of vitamin B12 deficiency. Vitamin B12 deficiency leads to high concentrations of MMA in plasma, serum and urine. MMA is much more suitable for diagnosing a subclinical vitamin deficiency than the measurement of vitamin B12 itself, which is bound to various  proteins in the blood and is significantly more unstable. Furthermore, MMA is a more sensitive biomarker, which indicates vitamin B12 deficiency earlier.

Vitamin B12 deficiency that remains untreated for years can lead to many clinical symptoms. Neurological and physical disorders have been observed, as well as anaemia and pregnancy complications up to miscarriages. Together with vitamin B6 and folic acid, vitamin B12 also plays a role in Alzheimer’s disease. Therefore, early determination of the MMA level and early treatment can prevent certain diseases and irreversible neurological damages.

 

Product advantages

  • Clean-Up Tubes for fast processing of plasma and serum samples
  • Direct injection of urine samples after dilution
  • Chromatographic separation of succinic acid
  • 3PLUS1® Multilevel Calibrator Sets for plasma and urine

 

This Chromsystems kit allows the specific determination of methylmalonic acid in plasma, serum and urine. The use of special clean-up tubes enables removing interfering compounds fast and effectively. The isobaric succinic acid is separated chromatographically to prevent false-positive test results. A stable isotopically labelled internal standard is used to ensure reliable quantification. Matrix effects are minimised and the precision and robustness of the method are optimised. The human-based 3PLUS1® multilevel plasma and urine calibrators ensure high precision of the results.

Please see accessories for the additional urine upgrade set.

More Information
Number of Tests 400
Limit of quantification

Plasma/Serum: 3.5 µg/l
Urine: 86 µg/l

Linearity

Plasma/Serum: up to 600 µg/l
Urine: up to 9500 µg/l

Recovery Plasma/Serum: 104 %; Urine: 98 %
Intraassay

Plasma/Serum: CV = 2.3-2.5 %
Urine: 1.8-2.2 %

Interassay

Plasma/Serum: CV = 3.2-3.6 %
Urine: CV = 2.4-2.9 %

Analysis Time 3.5 min
Sample Preparation

Plasma/Serum:

  • Pipette 100 µl sample/calibrator/control into Clean-Up Tubes
  • Add 50 µl Internal Standard and mix (vortex) briefly
  • Centrifuge 10 min at 14 000 x g
  • Transfer filtrate into an autosampler vial

Urine:

  • Pipette 200 µl sample/calibrator/control into transparent reaction vials
  • Add 25 µl Internal Standard and mix 10 s (vortex)
  • Dilute sample with Dilution Buffer in an autosampler vial
Sample Stability At +2 to +8 °C up to 14 days, below -18 °C up to 2 months.
Specimen Plasma/Serum/Urine
Injection Volume 10 to 20 µl
Flow rate 0.7 ml/min
Column temperature ambient temperature (approx. 25 °C)
Gradient
0 min 100 % Mobile Phase A
2.5 min 0 % Mobile Phase A, 100 % Mobile Phase B
2.51-3.5 min 100 % Mobile Phase A
Ionisation ESI negative
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter Methylmalonic Acid (MMA)
  1. 64001 MMA Mobile Phase A LCMS
    Mobile Phase A
    Order no.: 64001

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  2. 64002 MMA Mobile Phase B LCMS
    Mobile Phase B
    Order no.: 64002

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  3. 64009 LCMS MMA rinsing solution plasma serum urine
    Rinsing Solution
    Order no.: 64009

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  4. 64044U MMA Internal Standard Urine LCMS
    Internal Standard Methylmalonic Acid in Plasma/Serum
    Order no.: 64004/P

     MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  5. 64008 MMA Clean Up Tubes Plasma LCMS
    MMA Clean-Up Tubes
    Order no.: 64008

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  1. 3PLUS1® Multilevel Plasma Calibrator Set Methylmalonic Acid
    3PLUS1® Multilevel Plasma Calibrator Set Methylmalonic Acid
    Order no.: 64028

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  2. 3PLUS1® Multilevel Urine Calibrator Set Methylmalonic Acid
    3PLUS1® Multilevel Urine Calibrator Set Methylmalonic Acid
    Order no.: 64029

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  1. MassCheck® Methylmalonic Acid Urine Controls
    MassCheck® Methylmalonic Acid Urine Controls
    Order no.: 0316/0317/0318

    MassChrom® Methylmalonic Acid in Urine - LC-MS/MS

    Details
  1. PEEK-encased Prefilter, 2 µm
    Details
  2. Analytical Column
    Analytical Column
    Order no.: 64100

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine

    Details
  3. Tuning Mix Methylmalonic Acid
    Tuning Mix Methylmalonic Acid
    Order no.: 64015

    MassChrom® Methylmalonic Acid in Plasma/Serum and Urine - LC-MS/MS

    Details
  4. Urine Upgrade Set for MMA
    Urine Upgrade Set for MMA
    Order no.: MMA Urine Set

    Upgrade for order no. 64000

    MassChrom® Methylmalonic Acid

    Details
  5. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
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