MassChrom® Methylmalonic Acid in Serum/Plasma and Urine - LC-MS/MS

Order No.: 64000, for 400 tests
Methylmalonic Acid (MMA)

Clean-up tubes for fast preparation of plasma and serum samples
Direct injection of urine samples after dilution
Chromatographic separation of succinic acid

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation

Methylmalonic Acid (MMA)

Clinical relevance

Methylmalonic acid (MMA) is an early, sensitive biomarker of vitamin B12 deficiency. Vitamin B12 deficiency leads to high concentrations of MMA in plasma, serum and urine. MMA is much more suitable for diagnosing a subclinical vitamin deficiency than the measurement of vitamin B12 itself, which is bound to various  proteins in the blood and is significantly more unstable. Furthermore, MMA is a more sensitive biomarker, which indicates vitamin B12 deficiency earlier.

Vitamin B12 deficiency that remains untreated for years can lead to many clinical symptoms. Neurological and physical disorders have been observed, as well as anaemia and pregnancy complications up to miscarriages. Together with vitamin B6 and folic acid, vitamin B12 also plays a role in Alzheimer’s disease. Therefore, early determination of the MMA level and early treatment can prevent certain diseases and irreversible neurological damages.


Product advantages

  • Clean-Up Tubes for fast processing of plasma and serum samples
  • Direct injection of urine samples after dilution
  • Chromatographic separation of succinic acid
  • 3PLUS1® Multilevel Calibrator Sets for plasma and urine


This Chromsystems kit allows the specific determination of methylmalonic acid in plasma, serum and urine. The use of special clean-up tubes enables removing interfering compounds fast and effectively. The isobaric succinic acid is separated chromatographically to prevent false-positive test results. A stable isotopically labelled internal standard is used to ensure reliable quantification. Matrix effects are minimised and the precision and robustness of the method are optimised. The human-based 3PLUS1® multilevel plasma and urine calibrators ensure high precision of the results.

Please see accessories for the additional urine upgrade set.

More Information
Method of Analysis LC-MS/MS
Number of Tests 400
Limit of quantification

Plasma/Serum: 3.5 µg/l
Urine: 86 µg/l


Plasma/Serum: up to 600 µg/l
Urine: up to 9500 µg/l

Recovery Plasma/Serum: 104 %; Urine: 98 %

Plasma/Serum: CV = 2.3-2.5 %
Urine: 1.8-2.2 %


Plasma/Serum: CV = 3.2-3.6 %
Urine: CV = 2.4-2.9 %

Analysis Time 3.5 min
Specimen Plasma/Serum/Urine
Sample Preparation


  • Pipette 100 µl sample/calibrator/control into Clean-Up Tubes
  • Add 50 µl Internal Standard and mix (vortex) briefly
  • Centrifuge 10 min at 14 000 x g
  • Transfer filtrate into an autosampler vial


  • Pipette 200 µl sample/calibrator/control into transparent reaction vials
  • Add 25 µl Internal Standard and mix 10 s (vortex)
  • Dilute sample with Dilution Buffer in an autosampler vial
Sample Stability At +2 to +8 °C up to 14 days, below -18 °C up to 2 months.
Injection Volume 10 to 20 µl
Flow rate 0.7 ml/min
Column temperature ambient temperature (approx. 25 °C)
0 min 100 % Mobile Phase A
2.5 min 0 % Mobile Phase A, 100 % Mobile Phase B
2.51-3.5 min 100 % Mobile Phase A
Ionisation ESI negative
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Methylmalonic Acid (MMA)
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