Catecholamines in Urine, Combined analysis - HPLC
Short analysis time < 6 min
Only 1 HPLC column and 1 Mobile Phase for 2020/COMBI and 6000/COMBI
The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.
- Short analysis time of less than 6 minutes
- No changes to setup are needed due to identical column and mobile phase
This kit enables the combined determination of catecholamines together with metanephrine in urine (Kit 2020/COMBI). It is an exceptionally sensitive and reliable method for detecting pheochromocytomas with an analysis time of less than 6 minutes. The highly selective separation column and the optimised mobile phase are used for the analysis of both catecholamines and metanephrines, so no change of the chromatographic system is required. The established specific sample preparation using solid phase extraction guarantees simple and safe handling and reproducible results.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||1 μg/l|
CV = 0.9-2.7 %
CV = 2.1-4.1 %
|Analysis Time||< 6 min|
|Injection Volume||10 µl|
|Flow rate||0.9 ml/min|
|Column temperature||30 °C|
For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used.
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Parameter||3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine|