25-OH-Vitamin D3/D2 in Serum/Plasma - HPLC

Clinical relevance

Vitamin D (cholecalciferol) is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.

25-OH-vitamin D₃ is the primary metabolite in the human body and the direct precursor of the physiologically active form 1.25-(OH)₂-vitamin D₃. First-line treatment for vitamin D deficiency is nutritional supplements with vitamin D₃ or vitamin D₂. Vitamin D₂ is easier to synthesise and is widely used as a supplement outside of the EU, and especially in the United States. Therefore, its determination in serum/plasma is also relevant.

Product advantages

• Combined analysis of vitamins D₃ and D₂
• Reliable separation
• Reliable quantification with a stable internal standard

This Chromsystems kit allows the simultaneous and reliable chromatographic determination of 25-OH-Vitamin D₃ and 25-OH-Vitamin D₂ on an isocratic HPLC system with UV detection. By means of efficient protein precipitation and selective solid phase extraction interfering components are removed. A non-physiological derivative of vitamin D is used as a stable internal standard to produce reliable quantification. This way, reproducible and reliable results can be produced at a low expenditure of cost and time.

Alternatively, for high sample throughput Chromsystems offers an online method with selective online solid phase extraction (order no. 38900/1000) as well as methods for analysis using mass spectrometry (order no. 62000).