25-OH-Vitamin D3/D2 in Serum/Plasma - HPLC
Vitamins D3 and D2 with one analysis
Quality controls and calibrator available
Vitamin D (cholecalciferol) is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.
25-OH-vitamin D3 is the primary metabolite in the human body and the direct precursor of the physiologically active form 1.25-(OH)2-vitamin D3. First-line treatment for vitamin D deficiency is nutritional supplements with vitamin D3 or vitamin D2. Vitamin D2 is easier to synthesise and is widely used as a supplement outside of the EU, and especially in the United States. Therefore, its determination in serum/plasma is also relevant.
- Combined analysis of vitamins D3 and D2
- Reliable separation
- Reliable quantification with a stable internal standard
This Chromsystems kit allows the simultaneous and reliable chromatographic determination of 25-OH-Vitamin D3 and 25-OH-Vitamin D2 on an isocratic HPLC system with UV detection. By means of efficient protein precipitation and selective solid phase extraction interfering components are removed. A non-physiological derivative of vitamin D is used as a stable internal standard to produce reliable quantification. This way, reproducible and reliable results can be produced at a low expenditure of cost and time.
Alternatively, for high sample throughput Chromsystems offers an online method with selective online solid phase extraction (order no. 38900/1000) as well as methods for analysis using mass spectrometry (order no. 62000).
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||
1.1 µg/l - 1.4 µg/l
|Linearity||up to at least 250 µg/l|
|Recovery||25-OH-Vitamin D3: 86 %; 25-OH-Vitamin D2: 87 %|
25-OH-Vitamin D3: CV 3.0 %
25-OH-Vitamin D3: CV 3.3 %
|Analysis Time||10 min|
|Sample Stability||At ambient temperature up to 3 days, at +2 to +8 °C up to 1 week. It is recommended to keep samples cool for transport. Freeze for longer storage (up to 1 year) at -20 °C.|
|Injection Volume||25 µl (10-50 µl)|
|Flow rate||0.7 ml/min|
|Column temperature||approx. 25 °C|
|Additional Info||Isocratic HPLC system with UV detector.|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Parameter||25-OH-Vitamin D2, 25-OH-Vitamin D3|
|Injection interval||12 min|
|Autosampler rinsing solution||Water:acetonitrile = 50:50|
3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62028