MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised), incl. Ado, dAdo, with 96 Well Filter Plates
62 analytes in one run including adenosine and 2'-deoxyadenosine
IVDR-certified
No derivatisation required
No analytical column required
Matrix-based controls in three concentration levels
Linearity set for determining the upper end of the MS/MS measuring range
Sample prep with 96 well filter plates
Assay validated according to IVDR (=> Declaration of Conformity)
Clinical relevance
This assay allows the routine analysis of amino acids, succinylacetone, free carnitine, acylcarnitines, lysophospholipids, nucleosides, creatine and creatine-related metabolites in dried blood spots (DBS) for the newborn screening of metabolic disorders such as amino acidopathies, organic acidurias, fatty acid oxidation disorders, and purine or creatine metabolism disorders by MS/MS.
It is intended for screening of newborns for inherited metabolic disorders within the first few days of life.
Product advantages
• 62 analytes in one run including adenosine and 2'-deoxyadenosine
• IVDR-certified
• No derivatisation required
• No analytical column required
• Matrix-based controls in three concentration levels
• Linearity set for determining the upper end of the MS/MS measuring range
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
| Method of Analysis | FIA-MS/MS |
|---|---|
| Number of Tests | 960 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | For the LLOQ and ULOQ values of all analytes please refer to the instruction manual. |
| Specimen | Dried blood |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. • Punch a dried blood spot disk out of the filter card into a 96 Well Filter Plate. |
| Run Time | 1.7 min |
| Injection Volume | 10 µL |
| Flow Rate | 20–600 µL/min |
| MS/MS Mode | MRM |
| Additional Info | No HPLC column is required. |
| Information | For the mass transitions of all analytes and internal standards, please refer to the instruction manual. |
| Parameters | => see complete list in „Parameters“ tab |
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Mobile PhaseOrder no.: 57001MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised) -
Rinsing SolutionOrder no.: 57007MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised) -
Internal Standard MixOrder no.: 57074MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
Internal Standard Mix Ado, dAdoOrder no.: 57474MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
Internal Standard Succinylacetone (non-derivatised)Order no.: 57044MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised) -
Extraction BufferOrder no.: 57078MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
Extraction Buffer SuccinylacetoneOrder no.: 57013MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
96 Well PlatesOrder no.: 57010MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised) -
96 Well Filter PlatesOrder no.: 57055MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
Protective sheets for 96 Well PlatesOrder no.: 55011MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised) -
MassCheck® Amino Acids, Acylcarnitines EXTENDED incl. Ado, dAdo Dried Blood Spot ControlsOrder no.: 0295/0296/0297MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
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Tuning Mix 1 Amino Acids, Acylcarnitines EXTENDEDOrder no.: 57097-1MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
Tuning Mix 2 Amino Acids, Acylcarnitines EXTENDEDOrder no.: 57097-2MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
Tuning Mix Succinylacetone (non-derivatised)Order no.: 57098MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised) -
Multilevel Dried Blood Spot Linearity Set incl. Ado, dAdoOrder no.: 57090MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised) -
Pierceable Heat Seals for 96 Well PlatesOrder no.: 57014MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
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Cross-slitted Adhesive Seals for 96 Well PlatesOrder no.: 57059MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
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MassCheck® Amino Acids, Acylcarnitines EXTENDED incl. Ado, dAdo Dried Blood Spot ControlsOrder no.: 0295/0296/0297MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
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Chromsystems Heat SealerOrder no.: 42740Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint
Clinical relevance
This assay allows the routine analysis of amino acids, succinylacetone, free carnitine, acylcarnitines, lysophospholipids, nucleosides, creatine and creatine-related metabolites in dried blood spots (DBS) for the newborn screening of metabolic disorders such as amino acidopathies, organic acidurias, fatty acid oxidation disorders, and purine or creatine metabolism disorders by MS/MS.
It is intended for screening of newborns for inherited metabolic disorders within the first few days of life.
Product advantages
• 62 analytes in one run including adenosine and 2'-deoxyadenosine
• IVDR-certified
• No derivatisation required
• No analytical column required
• Matrix-based controls in three concentration levels
• Linearity set for determining the upper end of the MS/MS measuring range
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
| Method of Analysis | FIA-MS/MS |
|---|---|
| Number of Tests | 960 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | For the LLOQ and ULOQ values of all analytes please refer to the instruction manual. |
| Specimen | Dried blood |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. • Punch a dried blood spot disk out of the filter card into a 96 Well Filter Plate. |
| Run Time | 1.7 min |
| Injection Volume | 10 µL |
| Flow Rate | 20–600 µL/min |
| MS/MS Mode | MRM |
| Additional Info | No HPLC column is required. |
| Information | For the mass transitions of all analytes and internal standards, please refer to the instruction manual. |
| Parameters | => see complete list in „Parameters“ tab |
-
Mobile PhaseOrder no.: 57001MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
-
Rinsing SolutionOrder no.: 57007MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
-
Internal Standard MixOrder no.: 57074MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
Internal Standard Mix Ado, dAdoOrder no.: 57474MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
Internal Standard Succinylacetone (non-derivatised)Order no.: 57044MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
-
Extraction BufferOrder no.: 57078MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
Extraction Buffer SuccinylacetoneOrder no.: 57013MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
96 Well PlatesOrder no.: 57010MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
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96 Well Filter PlatesOrder no.: 57055MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
Protective sheets for 96 Well PlatesOrder no.: 55011MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
-
MassCheck® Amino Acids, Acylcarnitines EXTENDED incl. Ado, dAdo Dried Blood Spot ControlsOrder no.: 0295/0296/0297MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
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Tuning Mix 1 Amino Acids, Acylcarnitines EXTENDEDOrder no.: 57097-1MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
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Tuning Mix 2 Amino Acids, Acylcarnitines EXTENDEDOrder no.: 57097-2MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
Tuning Mix Succinylacetone (non-derivatised)Order no.: 57098MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
-
Multilevel Dried Blood Spot Linearity Set incl. Ado, dAdoOrder no.: 57090MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
Pierceable Heat Seals for 96 Well PlatesOrder no.: 57014MassScreen® Amino Acids and Acylcarnitines from Dried Blood (non-derivatised)
-
Cross-slitted Adhesive Seals for 96 Well PlatesOrder no.: 57059MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
-
MassCheck® Amino Acids, Acylcarnitines EXTENDED incl. Ado, dAdo Dried Blood Spot ControlsOrder no.: 0295/0296/0297MassScreen® Amino Acids, Acylcarnitines EXTENDED from Dried Blood (non-derivatised)
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Chromsystems Heat SealerOrder no.: 42740
Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint