CDT in Serum - ternary gradient systems - HPLC

Order No.: 54030 & 54030/500
Parameters:
Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Sample preparation: just 2 pipetting steps
Long column lifetime
Low cost per test
Reference method for CDT analyses: currently HPLC

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Grouped product items
Product Name Qty
CDT in Serum - ternary gradient system - HPLC
Order No.: 54030
CDT in Serum - ternary gradient system - HPLC
Order No.: 54030/500

Asialotransferrin

Disialotransferrin

Pentasialotransferrin

Tetrasialotransferrin

Trisialotransferrin

Clinical relevance

Carbohydrate deficient transferrin (CDT) is regarded as the most specific laboratory marker of chronic alcohol abuse. Unlike otherbiochemical parameters such as γ-GT or MCV, false positive results caused by non-alcoholic liver diseases can be excluded. A daily intake of more than 60 g ethanol (about 0.75 l wine) over a period of two weeks elevates CDT values significantly. After approx. 2 weeks of alcohol abstention, the levels normalise again. This makes CDT an excellent parameter for controlling withdrawal treatment, forensic judgements or in occupational medicine.

 

Product advantages

  • Specific marker for chronic alcohol abuse
  • Long column lifetime
  • Traceable to the IFCC reference measurement procedure

This Chromsystems assay is designed for the fast and reliable determination of CDT in serum using HPLC with UV detection. The results are calculated as area percent of total transferrin, and, therefore, are not affected by variations of the total transferrin concentration. The optimised chromatographic separation of the transferrin isoforms also ensures the detection of genetic variants of transferrin.

This method is traceable to the IFCC reference measurement procedure (IFCC-RMP) and certified accordingly. It can therefore also be used to determine CDTIFCC. The IFCC certificate is available for customers in the secure download area.

Alternative methods using Pre-mixed Tubes or automated sample preparation for higher sample throughput are also available.

More Information
Method of Analysis HPLC
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation approx. 0.5 % Disialotransferrin
Upper Limit of Quantification up to at least 12 % Disialotransferrin
Intraassay CV ≤ 6.2 %
Interassay CV ≤ 3.5 %
Recovery 99 %
Specimen Serum
Sample Preparation
  • Place 200 µl serum into a reaction vial.
  • Add 100 µl reaction mix*, mix for 60 s (vortex).
  • Incubate 30 min at +2 bis +8 °C.
  • Centrifuge 10 min at ≥ 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 200 µl into the HPLC system.

* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 μl each per sample)

Run Time 20 min
Injection Volume 200 µl
Flow Rate 1.5 ml/min
Column Temperature ambient (~25 °C)
Wavelengths 460 nm
Additional Info

For the Chromsystems HPLC analysis of CDT in serum any ternary HPLC gradient system with UV detection is suitable.

Parameters Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin
The following components are included in the kit:
As a customer please login or register to gain full access.

Asialotransferrin

Disialotransferrin

Pentasialotransferrin

Tetrasialotransferrin

Trisialotransferrin

Clinical relevance

Carbohydrate deficient transferrin (CDT) is regarded as the most specific laboratory marker of chronic alcohol abuse. Unlike otherbiochemical parameters such as γ-GT or MCV, false positive results caused by non-alcoholic liver diseases can be excluded. A daily intake of more than 60 g ethanol (about 0.75 l wine) over a period of two weeks elevates CDT values significantly. After approx. 2 weeks of alcohol abstention, the levels normalise again. This makes CDT an excellent parameter for controlling withdrawal treatment, forensic judgements or in occupational medicine.

 

Product advantages

  • Specific marker for chronic alcohol abuse
  • Long column lifetime
  • Traceable to the IFCC reference measurement procedure

This Chromsystems assay is designed for the fast and reliable determination of CDT in serum using HPLC with UV detection. The results are calculated as area percent of total transferrin, and, therefore, are not affected by variations of the total transferrin concentration. The optimised chromatographic separation of the transferrin isoforms also ensures the detection of genetic variants of transferrin.

This method is traceable to the IFCC reference measurement procedure (IFCC-RMP) and certified accordingly. It can therefore also be used to determine CDTIFCC. The IFCC certificate is available for customers in the secure download area.

Alternative methods using Pre-mixed Tubes or automated sample preparation for higher sample throughput are also available.

More Information
Method of Analysis HPLC
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation approx. 0.5 % Disialotransferrin
Upper Limit of Quantification up to at least 12 % Disialotransferrin
Intraassay CV ≤ 6.2 %
Interassay CV ≤ 3.5 %
Recovery 99 %
Specimen Serum
Sample Preparation
  • Place 200 µl serum into a reaction vial.
  • Add 100 µl reaction mix*, mix for 60 s (vortex).
  • Incubate 30 min at +2 bis +8 °C.
  • Centrifuge 10 min at ≥ 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 200 µl into the HPLC system.

* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 μl each per sample)

Run Time 20 min
Injection Volume 200 µl
Flow Rate 1.5 ml/min
Column Temperature ambient (~25 °C)
Wavelengths 460 nm
Additional Info

For the Chromsystems HPLC analysis of CDT in serum any ternary HPLC gradient system with UV detection is suitable.

Parameters Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin
The following components are included in the kit:
As a customer please login or register to gain full access.