Catecholamines in Urine, Combined analysis - HPLC
Short analysis time < 6 min
Easy pH adjustment through Neutralisation Buffer with integrated indicator
Only 1 HPLC column and 1 Mobile Phase for 2020-C and 6000-C
Assay validated according to IVDR (=> Declaration of Conformity)
Chromatogram for 6000-C (Catecholamines in Urine, Combined Analysis)
Chromatogram for 2020-C (Metanephrines in Urine, Combined Analysis)
using the same HPLC column and mobile phase as kit 6000-C
With kit 6000-C
Adrenaline (Epinephrine)
Dopamine
Noradrenaline (Norepinephrine)
With kit 2020-C, using the same HPLC column and mobile phase as kit 6000-C
3-Methoxytyramine
Metanephrine
Normetanephrine
Clinical relevance
This Chromsystems assay allows the simultaneous quantitative determination of noradrenaline (syn. norepinephrine), adrenaline (syn. epinephrine), and dopamine in human urine samples by HPLC.
It is intended to be used for patients in whom the urinary levels of noradrenaline, adrenaline and dopamine are of clinical importance, primarily as an aid to diagnosis and monitoring of suspected pheochromocytoma and paraganglioma.
Product advantages
• Short analysis time of less than 6 minutes
• Easy pH adjustment through Neutralisation Buffer with integrated indicator
• Only 1 HPLC column and 1 mobile phase for 2020-C and 6000-C => No changes to setup are needed due to identical column and mobile phase.
Assay characteristics
Sample preparation consists of an efficient solid-phase extraction. The adjustment of the pH to ensure complete extraction is checked based on the colour change of an indicator. The use of a specific internal standard ensures high precision and reliability in quantitation of the analytes. The analytes and the internal standard are then chromatographically separated in a single isocratic HPLC run and quantified using an electrochemical detector (ECD).
Combined analysis
The same HPLC column and mobile phase can be used for both catecholamine (2020-C) and metanephrine (6000-C) analysis.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline/Epinephrine: 2.7 µg/L – 1000 µg/L |
| Specimen | 24-hour urine. Alternatively spot urine may also be used. In this case, results must be related to creatinine. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Buffering and pH-adjustment of sample/calibrator/control
Sample extraction with Sample Clean Up Columns
Continued sample preparation prior to injection
|
| Run Time | 6 min |
| Injection Volume | 10 µL |
| Flow Rate | 0.9 mL/min |
| Column Temperature | 30 °C |
| Potential | approx. +580 mV to +640 mV |
| Additional Info | For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used. |
| Parameters | Adrenaline (Epinephrine), Dopamine, Noradrenaline (Norepinephrine) |
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Mobile PhaseOrder no.: 2031/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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Stabilisation ReagentOrder no.: 6010/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
Chromatogram for 6000-C (Catecholamines in Urine, Combined Analysis)
Chromatogram for 2020-C (Metanephrines in Urine, Combined Analysis)
using the same HPLC column and mobile phase as kit 6000-C
With kit 6000-C
Adrenaline (Epinephrine)
Dopamine
Noradrenaline (Norepinephrine)
With kit 2020-C, using the same HPLC column and mobile phase as kit 6000-C
3-Methoxytyramine
Metanephrine
Normetanephrine
Clinical relevance
This Chromsystems assay allows the simultaneous quantitative determination of noradrenaline (syn. norepinephrine), adrenaline (syn. epinephrine), and dopamine in human urine samples by HPLC.
It is intended to be used for patients in whom the urinary levels of noradrenaline, adrenaline and dopamine are of clinical importance, primarily as an aid to diagnosis and monitoring of suspected pheochromocytoma and paraganglioma.
Product advantages
• Short analysis time of less than 6 minutes
• Easy pH adjustment through Neutralisation Buffer with integrated indicator
• Only 1 HPLC column and 1 mobile phase for 2020-C and 6000-C => No changes to setup are needed due to identical column and mobile phase.
Assay characteristics
Sample preparation consists of an efficient solid-phase extraction. The adjustment of the pH to ensure complete extraction is checked based on the colour change of an indicator. The use of a specific internal standard ensures high precision and reliability in quantitation of the analytes. The analytes and the internal standard are then chromatographically separated in a single isocratic HPLC run and quantified using an electrochemical detector (ECD).
Combined analysis
The same HPLC column and mobile phase can be used for both catecholamine (2020-C) and metanephrine (6000-C) analysis.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline/Epinephrine: 2.7 µg/L – 1000 µg/L |
| Specimen | 24-hour urine. Alternatively spot urine may also be used. In this case, results must be related to creatinine. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Buffering and pH-adjustment of sample/calibrator/control
Sample extraction with Sample Clean Up Columns
Continued sample preparation prior to injection
|
| Run Time | 6 min |
| Injection Volume | 10 µL |
| Flow Rate | 0.9 mL/min |
| Column Temperature | 30 °C |
| Potential | approx. +580 mV to +640 mV |
| Additional Info | For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used. |
| Parameters | Adrenaline (Epinephrine), Dopamine, Noradrenaline (Norepinephrine) |
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Mobile PhaseOrder no.: 2031/COMBI
Combined analysis of catecholamines/metanephrines in urine - HPLC
-
Stabilisation ReagentOrder no.: 6010/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
-
HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC