Vitamins B1 and B6 in Whole Blood, with 96 Well Filter Plates – UHPLC Reagent Kit
UHPLC separation in 3 min
Specific internal standard for each parameter
No post-column derivatisation required
One sample preparation and one run for both parameters
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746


Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
Clinical relevance
This assay allows the quantitative determination of the physiologically active forms of vitamin B1, thiamine pyrophosphate, and of vitamin B6, pyridoxal 5‘-phosphate, in human whole blood samples.
It is intended to be used for for screening and/or monitoring of vitamin B1 and/or B6 levels where indicated
• in patients with suspected vitamin B1 and/or B6 deficiency,
• in patients with suspected vitamin B1 and/or B6 excess,
• in patients under vitamin B1 and/or B6 supplementation therapy.
Product advantages
• UHPLC separation in 3 min
• Specific internal standard for each parameter
• No post-column derivatisation required
• One sample preparation and one run for both parameters
Assay characteristics
Thiamine pyrophosphate (TPP) and pyridoxal 5’-phosphate (PLP) can be determined in one run, with one sample preparation and without complex post-column derivatisation. Sample preparation is restricted to a single precipitation step and subsequent derivatisation and is possible with Pre-mixed Neutralisation Tubes (assay 52952/UHPLC) or 96 Well Filter Plates (assay 52952/UHPLC/F). Chromatographic separation is carried out with two Mobile Phases by UHPLC. The derivatised analytes are determined by fluorescence detection with wavelength switching. The use of two internal standards assures high precision and safety in quantifying the analytes.
Two types of kits available
Basic Kit
• Only sample preparation reagents and plates/seals included
• No excess quantities of mobile phase and calibrators
52052-F-BK (U)HPLC Basic Kit |
|
52744 | Internal Standard Mix, 50 ml (1x) |
52705 | Extraction Reagent, 75 ml (2x) |
52706 | Prep Solution, 25 ml (3x) |
52707 | Finisher 1, 5 x 0.25 ml (lyoph.) (2x) |
52708 | Finisher 2, 50 ml (1x) |
52709 | Dilution Buffer, 100 ml (1x) |
52057 | 96 Well Filter Plates, 5 pcs. (2x) |
52058 | Collection Plates, 10 pcs. (2x) |
52059 | Pierceable Adhesive Seals, 5 pcs. (2x) |
Reagent Kit
• Mobile phase and calibrator included
• UHPLC Column also included
52752/UHPLC/F Reagent Kit |
|
52744 | Internal Standard Mix, 50 ml (1x) |
52705 | Extraction Reagent, 75 ml (2x) |
52706 | Prep Solution, 25 ml (3x) |
52707 | Finisher 1, 5 x 0.25 ml (lyoph.) (2x) |
52708 | Finisher 2, 50 ml (1x) |
52709 | Dilution Buffer, 100 ml (1x) |
52057 | 96 Well Filter Plates, 5 pcs. (2x) |
52058 | Collection Plates, 10 pcs. (2x) |
52059 | Pierceable Adhesive Seals, 5 pcs. (2x) |
52911 | Mobile Phase A (for UHPLC), 1000 ml (2x) |
52922 | Mobile Phase B (for UHPLC), 1000 ml (1x) |
52003 | Whole Blood Calibration Standard, 5 x 1.0 ml (lyoph.) (5x) |
52210 | UHPLC Column, equilibrated, with test chromatogram, 1 pc. (1x) |
Method of Analysis | UHPLC |
---|---|
Number of Tests | 960 |
Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendix of the instruction manual. |
Lower and Upper Limit of Quantitation | TPP 2.9 – 750 µg/l (whole blood) |
Specimen | Whole Blood |
Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
Run Time | 3 min |
Injection Volume | 5 μl |
Flow Rate | 0.7 ml/min |
Column Temperature | +20 to +25 °C |
Wavelengths | Start EX 320 nm, EM 415 nm, |
Additional Info | Any common UHPLC system with binary pump and programmable fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
Vitamin B1 and Vitamin B6 in Whole Blood – UHPLC Reagent Kit (52952/UHPLC/F)
For 960 tests
Order No. | Reagent Kit components (Quantities in kit) |
52911 | Mobile Phase A (for UHPLC), 1000 ml (2x) |
52922 | Mobile Phase B (for UHPLC), 1000 ml (1x) |
52003 | Whole Blood Calibration Standard, 5 x 1.0 ml (lyoph.) (5x) |
52744 | Internal Standard Mix, 50 ml (1x) |
52705 | Extraction Reagent, 75 ml (2x) |
52706 | Prep Solution, 25 ml (3x) |
52707 | Finisher 1, 5 x 0.25 ml (lyoph.) (2x) |
52708 | Finisher 2, 50 ml (1x) |
52709 | Dilution Buffer, 100 ml (1x) |
52057 | 96 Well Filter Plates, 5 pcs. (2x) |
52058 | Collection Plates, 10 pcs. (2x) |
52059 | Pierceable Adhesive Seals, 5 pcs. (2x) |
52210 | UHPLC Column, equilibrated, with test chromatogram, 1 pc. (1x) |
Order No. | Required components (not included in the Reagent Kit) |
0165 | Vitamin B1/B6 Whole Blood Control, Level I, 5 x 2.0 ml (lyoph.) |
0167 | Vitamin B1/B6 Whole Blood Control, Level II, 5 x 2.0 ml (lyoph.) |
Order No. | General lab equipment (non-CE/IVD products) |
15060 | Prefilter Housing, 1 pc. |
15061 | Prefilter, 0.5 µm, 5 pcs. |
-
Vitamins B1/B6 Whole Blood Calibration StandardOrder no.: 52003
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Internal Standard MixOrder no.: 52744
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Extraction ReagentOrder no.: 52705
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Dilution BufferOrder no.: 52709
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
96 Well Filter PlatesOrder no.: 52057
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 52059
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Vitamins B1/B6 Whole Blood Calibration StandardOrder no.: 52003
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC


Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
Clinical relevance
This assay allows the quantitative determination of the physiologically active forms of vitamin B1, thiamine pyrophosphate, and of vitamin B6, pyridoxal 5‘-phosphate, in human whole blood samples.
It is intended to be used for for screening and/or monitoring of vitamin B1 and/or B6 levels where indicated
• in patients with suspected vitamin B1 and/or B6 deficiency,
• in patients with suspected vitamin B1 and/or B6 excess,
• in patients under vitamin B1 and/or B6 supplementation therapy.
Product advantages
• UHPLC separation in 3 min
• Specific internal standard for each parameter
• No post-column derivatisation required
• One sample preparation and one run for both parameters
Assay characteristics
Thiamine pyrophosphate (TPP) and pyridoxal 5’-phosphate (PLP) can be determined in one run, with one sample preparation and without complex post-column derivatisation. Sample preparation is restricted to a single precipitation step and subsequent derivatisation and is possible with Pre-mixed Neutralisation Tubes (assay 52952/UHPLC) or 96 Well Filter Plates (assay 52952/UHPLC/F). Chromatographic separation is carried out with two Mobile Phases by UHPLC. The derivatised analytes are determined by fluorescence detection with wavelength switching. The use of two internal standards assures high precision and safety in quantifying the analytes.
Two types of kits available
Basic Kit
• Only sample preparation reagents and plates/seals included
• No excess quantities of mobile phase and calibrators
52052-F-BK (U)HPLC Basic Kit |
|
52744 | Internal Standard Mix, 50 ml (1x) |
52705 | Extraction Reagent, 75 ml (2x) |
52706 | Prep Solution, 25 ml (3x) |
52707 | Finisher 1, 5 x 0.25 ml (lyoph.) (2x) |
52708 | Finisher 2, 50 ml (1x) |
52709 | Dilution Buffer, 100 ml (1x) |
52057 | 96 Well Filter Plates, 5 pcs. (2x) |
52058 | Collection Plates, 10 pcs. (2x) |
52059 | Pierceable Adhesive Seals, 5 pcs. (2x) |
Reagent Kit
• Mobile phase and calibrator included
• UHPLC Column also included
52752/UHPLC/F Reagent Kit |
|
52744 | Internal Standard Mix, 50 ml (1x) |
52705 | Extraction Reagent, 75 ml (2x) |
52706 | Prep Solution, 25 ml (3x) |
52707 | Finisher 1, 5 x 0.25 ml (lyoph.) (2x) |
52708 | Finisher 2, 50 ml (1x) |
52709 | Dilution Buffer, 100 ml (1x) |
52057 | 96 Well Filter Plates, 5 pcs. (2x) |
52058 | Collection Plates, 10 pcs. (2x) |
52059 | Pierceable Adhesive Seals, 5 pcs. (2x) |
52911 | Mobile Phase A (for UHPLC), 1000 ml (2x) |
52922 | Mobile Phase B (for UHPLC), 1000 ml (1x) |
52003 | Whole Blood Calibration Standard, 5 x 1.0 ml (lyoph.) (5x) |
52210 | UHPLC Column, equilibrated, with test chromatogram, 1 pc. (1x) |
Method of Analysis | UHPLC |
---|---|
Number of Tests | 960 |
Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendix of the instruction manual. |
Lower and Upper Limit of Quantitation | TPP 2.9 – 750 µg/l (whole blood) |
Specimen | Whole Blood |
Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
Run Time | 3 min |
Injection Volume | 5 μl |
Flow Rate | 0.7 ml/min |
Column Temperature | +20 to +25 °C |
Wavelengths | Start EX 320 nm, EM 415 nm, |
Additional Info | Any common UHPLC system with binary pump and programmable fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
Vitamin B1 and Vitamin B6 in Whole Blood – UHPLC Reagent Kit (52952/UHPLC/F)
For 960 tests
Order No. | Reagent Kit components (Quantities in kit) |
52911 | Mobile Phase A (for UHPLC), 1000 ml (2x) |
52922 | Mobile Phase B (for UHPLC), 1000 ml (1x) |
52003 | Whole Blood Calibration Standard, 5 x 1.0 ml (lyoph.) (5x) |
52744 | Internal Standard Mix, 50 ml (1x) |
52705 | Extraction Reagent, 75 ml (2x) |
52706 | Prep Solution, 25 ml (3x) |
52707 | Finisher 1, 5 x 0.25 ml (lyoph.) (2x) |
52708 | Finisher 2, 50 ml (1x) |
52709 | Dilution Buffer, 100 ml (1x) |
52057 | 96 Well Filter Plates, 5 pcs. (2x) |
52058 | Collection Plates, 10 pcs. (2x) |
52059 | Pierceable Adhesive Seals, 5 pcs. (2x) |
52210 | UHPLC Column, equilibrated, with test chromatogram, 1 pc. (1x) |
Order No. | Required components (not included in the Reagent Kit) |
0165 | Vitamin B1/B6 Whole Blood Control, Level I, 5 x 2.0 ml (lyoph.) |
0167 | Vitamin B1/B6 Whole Blood Control, Level II, 5 x 2.0 ml (lyoph.) |
Order No. | General lab equipment (non-CE/IVD products) |
15060 | Prefilter Housing, 1 pc. |
15061 | Prefilter, 0.5 µm, 5 pcs. |
-
Vitamins B1/B6 Whole Blood Calibration StandardOrder no.: 52003
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Internal Standard MixOrder no.: 52744
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Extraction ReagentOrder no.: 52705
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Dilution BufferOrder no.: 52709
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
96 Well Filter PlatesOrder no.: 52057
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 52059
Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC/UHPLC
-
Vitamins B1/B6 Whole Blood Calibration StandardOrder no.: 52003
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC