Sample Prep Set Catecholamines, free Metanephrines, Serotonin with Sample Clean Up Columns
7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Validated according to IVDR (=> Declaration of Conformity)
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With Sample Clean Up Columns
Clinical relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.
Product advantages
- 7 analytes in one single run
- Efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using 96 SPE well plates (80600/96 & 80600/480).
This Sample Prep Set encompasses all required components for the sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline (norepinephrine): 4.4 µg/l – 1600 µg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 4.0 min |
| Injection Volume | ≤ 10 µl |
| Gradient | binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Adrenaline (Epinephrine), Dopamine, Free 3-Methoxytyramine, Free Metanephrine, Free Normetanephrine, Noradrenaline (Norepinephrine), Serotonin |
80600/C Sample Prep Set Catecholamines, free Metanephrines, Serotonin with Sample Clean Up Columns
For 96 tests
| Order No. | Sample Prep Set components (Quantities in the set) |
| 80604 | Internal Standard Mix, 1x5 ml (1x) |
| 80605 | Neutralisation Buffer, 60 ml (1x) |
| 80606 | Wash Buffer 1, 90 ml (1x) |
| 80607 | Wash Buffer 2, 90 ml (2x) |
| 80608 | Elution Buffer, 50 ml (1x) |
| 80609 | Dilution Buffer, 50 ml (1x) |
| 80055 | Sample Clean Up Columns Mass Chrom® Biogenic Amines/Metabolites in Urine, 96 pcs. (1x) |
| Order No. | Required components (not included in the sample prep set) |
| 80000 |
Chromatographic Platform Starter Set, 1 pc. |
| 80739 | 6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, Serotonin, 7 x 2.0 ml (lyoph.) |
| 0376 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level I, 5 x 2.0 ml (lyoph.) |
| 0377 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level II, 5 x 2.0 ml (lyoph.) |
| 80603 | Adjustment Reagent, 10 ml |
| 80615 | Tuning Mix, Analytes and Internal Standards, 1 ml |
| Order No. | Lab equipment |
| 33007 | Tubes for sample preparation, 50 pcs. |
| J0601 | Autosampler Vials, screw neck, amber glass, 1.5 mL, 100 pcs. |
| J0504 | PE Screw-on Caps, rubber/PTFE septa, 9 mm, 100 pcs. |
| J0410 | PP Screw-on Caps, pierceable silicone/PTFE septa, 1.0 mm |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 1 pc. |
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Internal Standard MixOrder No.: 80604MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
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Sample Clean Up Columns Mass Chrom® Biogenic Amines/Metabolites in UrineOrder No.: 80055MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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MassChrom® Biogenic Amines/Metabolites in Urine – Starter Set Chromatographic PlatformOrder No.: 80000Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
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Tuning Mix Catecholamines, free Metanephrines, SerotoninOrder No.: 80615MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With Sample Clean Up Columns
Clinical relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.
Product advantages
- 7 analytes in one single run
- Efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using 96 SPE well plates (80600/96 & 80600/480).
This Sample Prep Set encompasses all required components for the sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline (norepinephrine): 4.4 µg/l – 1600 µg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 4.0 min |
| Injection Volume | ≤ 10 µl |
| Gradient | binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Adrenaline (Epinephrine), Dopamine, Free 3-Methoxytyramine, Free Metanephrine, Free Normetanephrine, Noradrenaline (Norepinephrine), Serotonin |
80600/C Sample Prep Set Catecholamines, free Metanephrines, Serotonin with Sample Clean Up Columns
For 96 tests
| Order No. | Sample Prep Set components (Quantities in the set) |
| 80604 | Internal Standard Mix, 1x5 ml (1x) |
| 80605 | Neutralisation Buffer, 60 ml (1x) |
| 80606 | Wash Buffer 1, 90 ml (1x) |
| 80607 | Wash Buffer 2, 90 ml (2x) |
| 80608 | Elution Buffer, 50 ml (1x) |
| 80609 | Dilution Buffer, 50 ml (1x) |
| 80055 | Sample Clean Up Columns Mass Chrom® Biogenic Amines/Metabolites in Urine, 96 pcs. (1x) |
| Order No. | Required components (not included in the sample prep set) |
| 80000 |
Chromatographic Platform Starter Set, 1 pc. |
| 80739 | 6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, Serotonin, 7 x 2.0 ml (lyoph.) |
| 0376 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level I, 5 x 2.0 ml (lyoph.) |
| 0377 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level II, 5 x 2.0 ml (lyoph.) |
| 80603 | Adjustment Reagent, 10 ml |
| 80615 | Tuning Mix, Analytes and Internal Standards, 1 ml |
| Order No. | Lab equipment |
| 33007 | Tubes for sample preparation, 50 pcs. |
| J0601 | Autosampler Vials, screw neck, amber glass, 1.5 mL, 100 pcs. |
| J0504 | PE Screw-on Caps, rubber/PTFE septa, 9 mm, 100 pcs. |
| J0410 | PP Screw-on Caps, pierceable silicone/PTFE septa, 1.0 mm |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 1 pc. |
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Internal Standard MixOrder No.: 80604
MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
-
Sample Clean Up Columns Mass Chrom® Biogenic Amines/Metabolites in UrineOrder No.: 80055
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
MassChrom® Biogenic Amines/Metabolites in Urine – Starter Set Chromatographic PlatformOrder No.: 80000
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
-
Tuning Mix Catecholamines, free Metanephrines, SerotoninOrder No.: 80615
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS