Sample Prep Set Catecholamines, free Metanephrines, Serotonin with Sample Clean Up Columns

Order No.: 80600/C, for 96 tests
Parameters:
3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin

7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

Adrenaline (epinephrine)

Noradrenaline (norepinephrine)

Dopamine

Free metanephrine

Free normetanephrine

Free 3-methoxytyramine

Serotonin

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With Sample Clean Up Columns

 

Clinical relevance

This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.

It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.

 

Product advantages

  • 7 analytes in one single run
  • Efficient sample prep
    Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available

Alternatively, there is also a method available using 96 SPE well plates (80600/96 & 80600/480).

 

This Sample Prep Set encompasses all required components for the sample preparation.

In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

Noradrenaline (norepinephrine): 4.00 µg/l – 1600 µg/l
Adrenaline (epinephrine): 2.50 µg/l – 1600 µg/l
Dopamine: 13.5 µg/l – 3000 µg/l
Normetanephrine: 3.50 µg/l – 5000 µg/l
Metanephrine: 2.00 µg/l – 5000 µg/l
3-Methoxytyramine: 3.00 µg/l – 2600 µg/l
Serotonin: 2.00 µg/l – 5000 µg/l

Different systems might show different performance data.

Specimen 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual.
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 200 µl of the sample/calibrator/MassCheck® control into each Sample Clean Up Column.
  • Add 50 µl Internal Standard Mix and 600 µl Neutralisation Buffer.
  • Check for correct colour.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1 and centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2 and centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Put Sample Clean Up Columns on new glass collection vials (e.g. glass autosampler vials).
  • Add 500 µl Elution Buffer and centrifuge 2 min at 100 x g.
  • Add 500 µl Dilution Buffer to each glass vial.
  • Seal each glass vial.
  • Shortly vortex each glass vial and transfer vials to autosampler.
  • Inject up to 10 µl.
Run Time 4 min
Injection Volume ≤ 10 µl
Ionisation ESI positive
MS/MS Mode MRM
Parameters 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

Adrenaline (epinephrine)

Noradrenaline (norepinephrine)

Dopamine

Free metanephrine

Free normetanephrine

Free 3-methoxytyramine

Serotonin

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With Sample Clean Up Columns

 

Clinical relevance

This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.

It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.

 

Product advantages

  • 7 analytes in one single run
  • Efficient sample prep
    Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available

Alternatively, there is also a method available using 96 SPE well plates (80600/96 & 80600/480).

 

This Sample Prep Set encompasses all required components for the sample preparation.

In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

Noradrenaline (norepinephrine): 4.00 µg/l – 1600 µg/l
Adrenaline (epinephrine): 2.50 µg/l – 1600 µg/l
Dopamine: 13.5 µg/l – 3000 µg/l
Normetanephrine: 3.50 µg/l – 5000 µg/l
Metanephrine: 2.00 µg/l – 5000 µg/l
3-Methoxytyramine: 3.00 µg/l – 2600 µg/l
Serotonin: 2.00 µg/l – 5000 µg/l

Different systems might show different performance data.

Specimen 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual.
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 200 µl of the sample/calibrator/MassCheck® control into each Sample Clean Up Column.
  • Add 50 µl Internal Standard Mix and 600 µl Neutralisation Buffer.
  • Check for correct colour.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1 and centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2 and centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Put Sample Clean Up Columns on new glass collection vials (e.g. glass autosampler vials).
  • Add 500 µl Elution Buffer and centrifuge 2 min at 100 x g.
  • Add 500 µl Dilution Buffer to each glass vial.
  • Seal each glass vial.
  • Shortly vortex each glass vial and transfer vials to autosampler.
  • Inject up to 10 µl.
Run Time 4 min
Injection Volume ≤ 10 µl
Ionisation ESI positive
MS/MS Mode MRM
Parameters 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.