Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate
7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Validated according to IVDR (=> Declaration of Conformity)
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480)
Clinical relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.
Product advantages
- 7 analytes in one single run
- Efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).
This Sample Prep Set encompasses all required components for the sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline (norepinephrine): 4.4 µg/l – 1600 µg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 4.0 min |
| Injection Volume | ≤ 10 µl |
| Gradient | binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Adrenaline (Epinephrine), Dopamine, Free 3-Methoxytyramine, Free Metanephrine, Free Normetanephrine, Noradrenaline (Norepinephrine), Serotonin |
80600/480 Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate
For 480 tests
| Order No. | Sample Prep Set components (Quantities in the set) |
| 80604/5 | Internal Standard Mix, 5x5 ml (1x) |
| 80675 | Neutralisation Buffer, 300 ml (1x) |
| 80676 | Wash Buffer 1, 450 ml (1x) |
| 80677 | Wash Buffer 2, 500 ml (2x) |
| 80678 | Elution Buffer, 250 ml (1x) |
| 80679 | Dilution Buffer, 250 ml (1x) |
| 80056 | Collection Plates MassChrom® Biogenic Amines/Metabolites in Urine, 3 pcs. (2x) |
| 80057 | 96 SPE Well Plate MassChrom® Biogenic Amines/Metabolites in Urine, 1 pc. (5x) |
| 80059 | Pierceable adhesive seals for 96 Well Plates MassChrom® Biogenic Amines/Metabolites in Urine, 3 pcs. (3x) |
| Order No. | Required components (not included in the sample prep set) |
| 80000 |
Chromatographic Platform Starter Set, 1 pc. |
| 80739 | 6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, Serotonin, 7 x 2.0 ml (lyoph.) |
| 0376 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level I, 5 x 2.0 ml (lyoph.) |
| 0377 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level II, 5 x 2.0 ml (lyoph.) |
| 80603 | Adjustment Reagent, 10 ml |
| 80615 | Tuning Mix, Analytes and Internal Standards, 1 ml |
| Order No. | Lab equipment |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 1 pc. |
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Internal Standard Mix - 5 x 5 mlOrder No.: 80604/5MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Neutralisation BufferOrder No.: 80675MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Wash Buffer 1Order No.: 80676MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Wash Buffer 2Order No.: 80677MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Elution BufferOrder No.: 80678MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Dilution BufferOrder No.: 80679MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
-
-
Pierceable adhesive seals for 96 Well PlatesOrder No.: 80059MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
MassChrom® Biogenic Amines/Metabolites in Urine – Starter Set Chromatographic PlatformOrder No.: 80000Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
-
Tuning Mix Catecholamines, free Metanephrines, SerotoninOrder No.: 80615MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480)
Clinical relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.
Product advantages
- 7 analytes in one single run
- Efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).
This Sample Prep Set encompasses all required components for the sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline (norepinephrine): 4.4 µg/l – 1600 µg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 4.0 min |
| Injection Volume | ≤ 10 µl |
| Gradient | binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Adrenaline (Epinephrine), Dopamine, Free 3-Methoxytyramine, Free Metanephrine, Free Normetanephrine, Noradrenaline (Norepinephrine), Serotonin |
80600/480 Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate
For 480 tests
| Order No. | Sample Prep Set components (Quantities in the set) |
| 80604/5 | Internal Standard Mix, 5x5 ml (1x) |
| 80675 | Neutralisation Buffer, 300 ml (1x) |
| 80676 | Wash Buffer 1, 450 ml (1x) |
| 80677 | Wash Buffer 2, 500 ml (2x) |
| 80678 | Elution Buffer, 250 ml (1x) |
| 80679 | Dilution Buffer, 250 ml (1x) |
| 80056 | Collection Plates MassChrom® Biogenic Amines/Metabolites in Urine, 3 pcs. (2x) |
| 80057 | 96 SPE Well Plate MassChrom® Biogenic Amines/Metabolites in Urine, 1 pc. (5x) |
| 80059 | Pierceable adhesive seals for 96 Well Plates MassChrom® Biogenic Amines/Metabolites in Urine, 3 pcs. (3x) |
| Order No. | Required components (not included in the sample prep set) |
| 80000 |
Chromatographic Platform Starter Set, 1 pc. |
| 80739 | 6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, Serotonin, 7 x 2.0 ml (lyoph.) |
| 0376 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level I, 5 x 2.0 ml (lyoph.) |
| 0377 | MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Control, Level II, 5 x 2.0 ml (lyoph.) |
| 80603 | Adjustment Reagent, 10 ml |
| 80615 | Tuning Mix, Analytes and Internal Standards, 1 ml |
| Order No. | Lab equipment |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 1 pc. |
-
Internal Standard Mix - 5 x 5 mlOrder No.: 80604/5
MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Neutralisation BufferOrder No.: 80675
MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Wash Buffer 1Order No.: 80676
MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Wash Buffer 2Order No.: 80677
MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Elution BufferOrder No.: 80678
MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
Dilution BufferOrder No.: 80679
MassChrom® Catecholamines, free Metanephrines, Serotonin in Urine - LC-MS/MS
-
-
-
Pierceable adhesive seals for 96 Well PlatesOrder No.: 80059
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
MassChrom® Biogenic Amines/Metabolites in Urine – Starter Set Chromatographic PlatformOrder No.: 80000
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
-
Tuning Mix Catecholamines, free Metanephrines, SerotoninOrder No.: 80615
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder No.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder No.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS