Urine Upgrade Set for Methylmalonic Acid (MMA)

Order No.: MMA Urine Set, for 200 tests
Parameters:
Methylmalonic Acid (MMA)

Upgrade for order no. 64000
MassChrom® Methylmalonic Acid

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Grouped product items
Product Name Qty
3PLUS1® Multilevel Urine Calibrator Set Methylmalonic Acid
Order No.: 64029
Dilution Buffer Urine
Order No.: 64007
Internal Standard Methylmalonic Acid in Urine
Order No.: 64044/U
Reaction Vials, transparent, 1.5 ml
Order No.: 33006
MassCheck® Methylmalonic Acid Urine Control, Bi-Level (I + II)
Order No.: 0316

Methylmalonic Acid (MMA)

Urine Upgrade Set for 200 Tests

The following items are additionally required to 64000:
64029 3PLUS1® Multilevel Urine Calibrator Set Methylmalonic Acid (lyoph.), 4 x 2 ml
64007 Dilution Buffer Urine, 75 ml
64004/U Internal Standard Methylmalonic Acid in Urine, 5 ml
33006 Reaction Vials, transparent, 100 pcs.
0317/0318 MassCheck® Methylmalonic Acid Urine Controls (lyoph.), 5 x 2 ml

More Information
Method of Analysis LC-MS/MS
Number of Tests 200
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation

86 µg/l

Upper Limit of Quantification

up to at least 9500 µg/l

Recovery 98 %
Intraassay

CV = 1.3–2.7 %

Interassay

CV = 2.4–2.9 %

Specimen Urine
Sample Preparation
  • Pipette 200 µl sample/calibrator/MassCheck® control into reaction vials
  • Add 25 µl Internal Standard and mix 10 s (vortex)
  • Dilute sample with Dilution Buffer in an autosampler vial
  • Inject 10–20 µl into the LC-MS/MS system
Run Time 3.5 min
Injection Volume 10–20 µl
Flow Rate 0.7 ml/min
Column Temperature ambient temperature (approx. 25 °C)
Gradient
0 min 100 % Mobile Phase A
2.5 min 0 % Mobile Phase A, 100 % Mobile Phase B
2.51-3.5 min 100 % Mobile Phase A
Ionisation ESI negative
MS/MS Mode MRM
Parameters Methylmalonic Acid (MMA)
The following components are included in the kit:
General lab equipment (non-CE-IVD products), available from Chromsystems:
As a customer please login or register to gain full access.

Methylmalonic Acid (MMA)

Urine Upgrade Set for 200 Tests

The following items are additionally required to 64000:
64029 3PLUS1® Multilevel Urine Calibrator Set Methylmalonic Acid (lyoph.), 4 x 2 ml
64007 Dilution Buffer Urine, 75 ml
64004/U Internal Standard Methylmalonic Acid in Urine, 5 ml
33006 Reaction Vials, transparent, 100 pcs.
0317/0318 MassCheck® Methylmalonic Acid Urine Controls (lyoph.), 5 x 2 ml

More Information
Method of Analysis LC-MS/MS
Number of Tests 200
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation

86 µg/l

Upper Limit of Quantification

up to at least 9500 µg/l

Recovery 98 %
Intraassay

CV = 1.3–2.7 %

Interassay

CV = 2.4–2.9 %

Specimen Urine
Sample Preparation
  • Pipette 200 µl sample/calibrator/MassCheck® control into reaction vials
  • Add 25 µl Internal Standard and mix 10 s (vortex)
  • Dilute sample with Dilution Buffer in an autosampler vial
  • Inject 10–20 µl into the LC-MS/MS system
Run Time 3.5 min
Injection Volume 10–20 µl
Flow Rate 0.7 ml/min
Column Temperature ambient temperature (approx. 25 °C)
Gradient
0 min 100 % Mobile Phase A
2.5 min 0 % Mobile Phase A, 100 % Mobile Phase B
2.51-3.5 min 100 % Mobile Phase A
Ionisation ESI negative
MS/MS Mode MRM
Parameters Methylmalonic Acid (MMA)
The following components are included in the kit:
General lab equipment (non-CE-IVD products), available from Chromsystems:
As a customer please login or register to gain full access.