MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine – LC-MS/MS
Very high column stability
Validated according to GTFCh guidelines
One step sample prep
Reliable determination also near cut-off values


Ethyl glucuronide (EtG)
Ethyl sulfate (EtS)
Clinical relevance
Alcohol is one of the world’s most frequently consumed drugs and is quickly absorbed in the small intestine of humans. It is metabolized in the liver to acetaldehyde and then to acetic acid. The elimination is quite fast, subsequently, the detection period of the alcohol itself is often limited to approximately 12 hours. However, less than 0.1% of the consumed alcohol is glucurono- or sulpho-conjugated, which is excreted as ethyl glucuronide (EtG) and ethyl sulphate (EtS). Both are detectable for a sustained period in comparison to ethanol itself, making them reliable and specific biomarkers for the clarification of alcohol consumption. Thus, EtG and EtS close the gap between short-term markers such as ethanol and long-term markers such as CDT.
Product benefits
- Very high column stability
- Validated according to GTFCh guidelines
- One step sample preparation
- Reliable determination also near cut-off values
The MassChrom® kit from Chromsystems enables reliable and precise determination of EtG and EtS in urine. This assay has been comprehensively validated according to the guidelines of the German Society of Toxicological and Forensic Chemistry (GTFCh) for quality assurance and enables the determination of both parameters with one sample preparation and in one single analysis time of 6 minutes. The straightforward and efficient sample preparation consists of a single pipetting step that includes the instrument-specific dilution. The human matrix-based 6PLUS1® Multilevel Calibrator Set and the MassCheck® controls ensure the precision and accuracy of the results.
Number of Tests | 400 |
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Limit of quantification | EtG: 35 µg/l EtS: 20 µg/l |
Recovery | EtG: 97-100 %; EtS: 95-98 % |
Intraassay | CV < 3 % |
Interassay | CV < 4 % |
Analysis Time | 6 min |
Sample Preparation | Place 50 µl sample/calibrator/MassCheck® control in autosampler vial. Master Mixture |
Sample Stability | Samples are stable up to 5 days at +2 to +8°C. For longer storage up to 3 months keep samples frozen below -18°C. |
Specimen | Urine |
Injection Volume | up to 20 µl |
Flow rate | 0.5-1.0 ml/min |
Gradient | 0.00–3.00 min, 0 % Mobile Phase B |
Ionisation | ESI negative |
MS/MS-Mode | MRM |
Additional Info | It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width. |
Information | It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width. |
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
Method of Analysis | LC-MS/MS |
Parameter | Ethyl glucuronide (EtG), Ethyl sulfate (EtS) |
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Mobile Phase AOrder no.: 69001
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
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Mobile Phase BOrder no.: 69002
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine – LC-MS/MS
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Internal Standard MixOrder no.: 69004
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
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Dilution BufferOrder no.: 69005MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
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Rinsing SolutionOrder no.: 69009
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
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6PLUS1® Multilevel Urine Calibrator Set Ethyl Glucuronide, Ethyl SulfateOrder no.: 69039
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine – LC-MS/MS
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MassCheck® Ethyl Glucuronide, Ethyl Sulfate Urine ControlsOrder no.: 0367/0368/0369
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
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Analytical Column Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MSOrder no.: 69100
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
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Tuning Mix Ethyl Glucuronide, Ethyl SulfateOrder no.: 69015
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
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System Check SolutionOrder no.: 69012
MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS