MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine – LC-MS/MS

Order No.: 69000, for 400 tests
Parameters:
Ethyl glucuronide (EtG), Ethyl sulfate (EtS)

Very high column stability
Validated according to GTFCh guidelines
One step sample prep
Reliable determination also near cut-off values

Ethyl glucuronide (EtG)

Ethyl sulfate (EtS)

Clinical relevance

Alcohol is one of the world’s most frequently consumed drugs and is quickly absorbed in the small intestine of humans. It is metabolized in the liver to acetaldehyde and then to acetic acid. The elimination is quite fast, subsequently, the detection period of the alcohol itself is often limited to approximately 12 hours. However, less than 0.1% of the consumed alcohol is glucurono- or sulpho-conjugated, which is excreted as ethyl glucuronide (EtG) and ethyl sulphate (EtS). Both are detectable for a sustained period in comparison to ethanol itself, making them reliable and specific biomarkers for the clarification of alcohol consumption. Thus, EtG and EtS close the gap between short-term markers such as ethanol and long-term markers such as CDT.

 

Product benefits

  • Very high column stability
  • Validated according to GTFCh guidelines
  • One step sample preparation
  • Reliable determination also near cut-off values

 

The MassChrom® kit from Chromsystems enables reliable and precise determination of EtG and EtS in urine. This assay has been comprehensively validated according to the guidelines of the German Society of Toxicological and Forensic Chemistry (GTFCh) for quality assurance and enables the determination of both parameters with one sample preparation and in one single analysis time of 6 minutes. The straightforward and efficient sample preparation consists of a single pipetting step that includes the instrument-specific dilution. The human matrix-based 6PLUS1® Multilevel Calibrator Set and the MassCheck® controls ensure the precision and accuracy of the results.

More Information
Number of Tests 400
Limit of quantification EtG: 35 µg/l
EtS: 20 µg/l
Recovery EtG: 97-100 %; EtS: 95-98 %
Intraassay CV < 3 %
Interassay CV < 4 %
Analysis Time 6 min
Sample Preparation

Place 50 µl sample/calibrator/MassCheck® control in autosampler vial.
Dilute as required by the instrument with Master Mixture (minimum dilution of 1:5).
Vortex briefly.
Inject up to 20 µl into the LC-MS/MS system.

Master Mixture
Example for 100 samples and a target dilution of 1:10: Add 2.5 ml Internal Standard Mix to 42.5 ml Dilution Buffer. Add 450 µl of this mixture to 50 µl sample.

Sample Stability Samples are stable up to 5 days at +2 to +8°C. For longer storage up to 3 months keep samples frozen below -18°C.
Specimen Urine
Injection Volume up to 20 µl
Flow rate 0.5-1.0 ml/min
Gradient

0.00–3.00 min, 0 % Mobile Phase B
3.01–5.50 min, 100 % Mobile Phase B
5.51–6.00 min, 0 % Mobile Phase B

Ionisation ESI negative
MS/MS-Mode MRM
Additional Info

It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width.

Information It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter Ethyl glucuronide (EtG), Ethyl sulfate (EtS)
  1. Mobile Phase A
    Mobile Phase A
    Order no.: 69001

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

    Details
  2. Mobile Phase B
    Mobile Phase B
    Order no.: 69002

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine – LC-MS/MS

    Details
  3. Internal Standard Mix
    Internal Standard Mix
    Order no.: 69004

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

    Details
  4. 69005 LCMS dilution buffer ethyl glucuronide EtG ethyl sulfate EtS urine
    Dilution Buffer
    Order no.: 69005
    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
    Details
  5. 69009 LCMS rinsing solution ethyl glucuronide EtG ethyl sulfate EtS urine
    Rinsing Solution
    Order no.: 69009

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

    Details
  1. 6PLUS1® Multilevel Urine Calibrator Set Ethyl Glucuronide, Ethyl Sulfate
    6PLUS1® Multilevel Urine Calibrator Set Ethyl Glucuronide, Ethyl Sulfate
    Order no.: 69039

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine – LC-MS/MS

    Details
  1.  LCMS EtG EtS MassCheck Controls
    MassCheck® Ethyl Glucuronide, Ethyl Sulfate Urine Controls
    Order no.: 0367/0368/0369

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

    Details
  1. PEEK-encased Prefilter, 2 µm
    Details
  2. HPLC Column EtG EtS 69100
    Analytical Column Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS
    Order no.: 69100

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

    Details
  3. 69015 LCMS tuning mix ethyl glucuronide EtG ethyl sulfate EtS urine
    Tuning Mix Ethyl Glucuronide, Ethyl Sulfate
    Order no.: 69015

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

    Details
  4. 69012 LCMS system check solution ethyl glucuronide EtG ethyl sulfate EtS urine
    System Check Solution
    Order no.: 69012

    MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

    Details
  5. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
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