MassTox® Drugs of Abuse Testing in Urine with 96 Well Plates

Order No.: 96000/WP, for 400 tests
Parameters:
Amphetamines, Barbiturates, Benzodiazepines, Booster, Cannabinoids, Cocaine, Opiates/Opioids, Other drugs of abuse, Z-Drugs

More than 100 drugs and metabolites
Target Screening and quantitative confirmation within the same run possible
Sample prep with 96 well plates

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Amphetamines
Amphetamine
BDB
Butylone
2C-B
2C-I
Cathinone
MBDB
MDA
MDEA
MDMA
MDPV
Mephedrone
Methamphetamine
Methaqualone
Methylone
Methylphenidate
PMA
Ritalinic Acid
Benzodiazepines
Alprazolam
7-Aminoclonazepam
7-Aminoflunitrazepam
7-Aminonitrazepam
Bromazepam
Brotizolam
Chlordiazepoxide
Clobazam
Clonazepam
Demoxepam
Desalkylflurazepam
Desmethylflunitrazepam
Diazepam
Estazolam
Flunitrazepam
Flurazepam
Lorazepam
Lormetazepam
Medazepam
Midazolam
Nitrazepam
Norclobazam
Nordiazepam
α-OH-Alprazolam
3-OH-Bromazepam
α-OH-Midazolam
α-OH-Triazolam
Oxazepam
Prazepam
Temazepam
Triazolam
Opiates/Opioids
Acetylcodeine
Buprenorphine
Codeine
Dihydrocodeine
EDDP
Fentanyl
Hydrocodone
Hydromorphone
Meconin
Meperidine
Methadone
6-Monoacetylmorphine
Morphine
Naloxone
Naltrexone
Norbuprenorphine
Norcodeine
Norfentanyl
Normeperidine
Nortapentadol
Nortilidine
O-Desmethyltramadol
Oxycodone
Oxymorphone
Papaverine
Propoxyphene
Sufentanil
Tapentadol
Thebaine
Tilidine
Tramadol
Booster
Gabapentin
Pregabalin
Promethazine
Quetiapine
Cannabinoids
THC-COOH
Cocaine
Benzoylecgonine
Cocaethylene
Cocaine
Norcocaine
Z-Drugs
Zaleplon
Zolpidem
Zopiclone
Barbiturates
Allobarbital
Amobarbital
Barbital
Butalbital
Hexobarbital
Pentobarbital
Phenobarbital
Secbutabarbital
Secobarbital
Thiopental
Others
Ketamine
LSD
Mescaline
Norketamine
O-H-LSD
PCP

Clinical relevance

Drug abuse is one of the most pressing public health issues worldwide, including the use of both illegal drugs and prescription medicines. Cannabis is by far the most commonly consumed illegal drug in Europe. Distant runners-up are the stimulants cocaine, MDMA and amphetamines. Within the class of opioids, heroin continues to be the most widely abused drug.

Drug of abuse testing in forensic medicine can establish both illicit drug use and whether crimes were committed under the influence of drugs. During the treatment of drug addicts, it is also performed to verify the abstinence of illicit drugs during replacement and maintenance therapy. In occupational medicine, drug screening is used to check for abstinence and to determine if a patient is fit to drive. Accurate testing can also help to save lives when someone is intoxicated.

 

Product advantages

  • Assay includes 108 drugs and metabolites
  • Target screening and quantitative confirmation within the same run
  • Run time of 12 minutes
  • Simple sample preparation combined with high selectivity and sensitivity
  • Internal standards for 98 analytes
  • With reaction vials or with 96 well plate

 

MassTox® Drugs of Abuse Testing is an LC-MS/MS-based assay that helps to increase speed of analysis and eliminates the need for developing and maintaining in-house methods or specific QC materials. The test is available with reaction vials or 96 well plates and allows the target screening of 108 drugs and metabolites. The confirmatory analysis can also be performed within the same run. It provides a significantly higher selectivity and sensitivity than current immunoassays and is designed to work below cut-offs applied in forensic labs. Semi-quantitative statements are also possible.

The assay includes isotopically labelled internal standards for nearly all analytes and allows the separation of all substances in a single chromatographic separation of approximately 12 minutes. The high stability of the 6PLUS1® Multilevel Calibrator Set and MassCheck® controls with three levels ensure reliable results with highest precision. A specific urine control with a carefully selected set of glucuronide metabolites allows controlling the proper functioning of the hydrolysis process. This Hydrolysis Control can also be used in laboratories working with their own developed methods and enzymes.

More Information
Method of Analysis LC-MS/MS
Number of Tests 400
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation down to 13 ng/l
Upper Limit of Quantification for all analytes: from 0.25 to 30 x of cut-off value
Intraassay CV = 1–9 %
Interassay CV = 1–11 %
Recovery 85–115 %
Specimen Urine
Sample Preparation
  • Pipette 50 µl sample/calibrator/MassCheck® control into a well plate.
  • Add 10 µl Internal Standard Mix.
  • Add 40 µl Enzyme Buffer Mix, shake for 1 min.
  • Incubate for 120 minutes at 45 ° C.
  • Add 100 µl Precipitation Reagent, shake for 1 min and centrifuge for 5 min.
  • In a collection plate dilute 100 µl supernatant with 150 µl Dilution Buffer, shake for 1 min.
  • Inject ≤ 20 µl into the LC-MS/MS system.
Run Time 12 min
Injection Volume 2–20 μl
Flow Rate 0.4 ml/min
Column Temperature 30 °C
Ionisation ESI positive and negative
MS/MS Mode MRM
Parameters Amphetamines, Barbiturates, Benzodiazepines, Booster, Cannabinoids, Cocaine, Opiates/Opioids, Other drugs of abuse, Z-Drugs
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.
Amphetamines
Amphetamine
BDB
Butylone
2C-B
2C-I
Cathinone
MBDB
MDA
MDEA
MDMA
MDPV
Mephedrone
Methamphetamine
Methaqualone
Methylone
Methylphenidate
PMA
Ritalinic Acid
Benzodiazepines
Alprazolam
7-Aminoclonazepam
7-Aminoflunitrazepam
7-Aminonitrazepam
Bromazepam
Brotizolam
Chlordiazepoxide
Clobazam
Clonazepam
Demoxepam
Desalkylflurazepam
Desmethylflunitrazepam
Diazepam
Estazolam
Flunitrazepam
Flurazepam
Lorazepam
Lormetazepam
Medazepam
Midazolam
Nitrazepam
Norclobazam
Nordiazepam
α-OH-Alprazolam
3-OH-Bromazepam
α-OH-Midazolam
α-OH-Triazolam
Oxazepam
Prazepam
Temazepam
Triazolam
Opiates/Opioids
Acetylcodeine
Buprenorphine
Codeine
Dihydrocodeine
EDDP
Fentanyl
Hydrocodone
Hydromorphone
Meconin
Meperidine
Methadone
6-Monoacetylmorphine
Morphine
Naloxone
Naltrexone
Norbuprenorphine
Norcodeine
Norfentanyl
Normeperidine
Nortapentadol
Nortilidine
O-Desmethyltramadol
Oxycodone
Oxymorphone
Papaverine
Propoxyphene
Sufentanil
Tapentadol
Thebaine
Tilidine
Tramadol
Booster
Gabapentin
Pregabalin
Promethazine
Quetiapine
Cannabinoids
THC-COOH
Cocaine
Benzoylecgonine
Cocaethylene
Cocaine
Norcocaine
Z-Drugs
Zaleplon
Zolpidem
Zopiclone
Barbiturates
Allobarbital
Amobarbital
Barbital
Butalbital
Hexobarbital
Pentobarbital
Phenobarbital
Secbutabarbital
Secobarbital
Thiopental
Others
Ketamine
LSD
Mescaline
Norketamine
O-H-LSD
PCP

Clinical relevance

Drug abuse is one of the most pressing public health issues worldwide, including the use of both illegal drugs and prescription medicines. Cannabis is by far the most commonly consumed illegal drug in Europe. Distant runners-up are the stimulants cocaine, MDMA and amphetamines. Within the class of opioids, heroin continues to be the most widely abused drug.

Drug of abuse testing in forensic medicine can establish both illicit drug use and whether crimes were committed under the influence of drugs. During the treatment of drug addicts, it is also performed to verify the abstinence of illicit drugs during replacement and maintenance therapy. In occupational medicine, drug screening is used to check for abstinence and to determine if a patient is fit to drive. Accurate testing can also help to save lives when someone is intoxicated.

 

Product advantages

  • Assay includes 108 drugs and metabolites
  • Target screening and quantitative confirmation within the same run
  • Run time of 12 minutes
  • Simple sample preparation combined with high selectivity and sensitivity
  • Internal standards for 98 analytes
  • With reaction vials or with 96 well plate

 

MassTox® Drugs of Abuse Testing is an LC-MS/MS-based assay that helps to increase speed of analysis and eliminates the need for developing and maintaining in-house methods or specific QC materials. The test is available with reaction vials or 96 well plates and allows the target screening of 108 drugs and metabolites. The confirmatory analysis can also be performed within the same run. It provides a significantly higher selectivity and sensitivity than current immunoassays and is designed to work below cut-offs applied in forensic labs. Semi-quantitative statements are also possible.

The assay includes isotopically labelled internal standards for nearly all analytes and allows the separation of all substances in a single chromatographic separation of approximately 12 minutes. The high stability of the 6PLUS1® Multilevel Calibrator Set and MassCheck® controls with three levels ensure reliable results with highest precision. A specific urine control with a carefully selected set of glucuronide metabolites allows controlling the proper functioning of the hydrolysis process. This Hydrolysis Control can also be used in laboratories working with their own developed methods and enzymes.

More Information
Method of Analysis LC-MS/MS
Number of Tests 400
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation down to 13 ng/l
Upper Limit of Quantification for all analytes: from 0.25 to 30 x of cut-off value
Intraassay CV = 1–9 %
Interassay CV = 1–11 %
Recovery 85–115 %
Specimen Urine
Sample Preparation
  • Pipette 50 µl sample/calibrator/MassCheck® control into a well plate.
  • Add 10 µl Internal Standard Mix.
  • Add 40 µl Enzyme Buffer Mix, shake for 1 min.
  • Incubate for 120 minutes at 45 ° C.
  • Add 100 µl Precipitation Reagent, shake for 1 min and centrifuge for 5 min.
  • In a collection plate dilute 100 µl supernatant with 150 µl Dilution Buffer, shake for 1 min.
  • Inject ≤ 20 µl into the LC-MS/MS system.
Run Time 12 min
Injection Volume 2–20 μl
Flow Rate 0.4 ml/min
Column Temperature 30 °C
Ionisation ESI positive and negative
MS/MS Mode MRM
Parameters Amphetamines, Barbiturates, Benzodiazepines, Booster, Cannabinoids, Cocaine, Opiates/Opioids, Other drugs of abuse, Z-Drugs
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.