MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns - LC-MS/MS

Clinical relevance

Steroid hormones are structurally derived from cholesterol and play a role in a wide range of physiological processes, including carbohydrate metabolism, fat and protein metabolism as well as in stress response. They are secreted by the adrenal cortex, testes, ovaries, and during pregnancy by the placenta. They are transported through the bloodstream to the cells of various organs where they influence and regulate the organs’ physiological functions.

The determination of hormones is important for the diagnosis of a number of diseases such as adrenogenital syndrome, hyperaldosteronism, or tumours. By using LC-MS/MS, not only high sensitivity and specificity can be achieved, but also the efficient measurement of multiple analytes in a single run.

Product advantages

• 15 steroids – 2 panels – 1 analytical column
• One sample preparation for all parameters
• Each parameter safeguarded by its own internal standard

Assay characteristics

This Chromsystems assay allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient solid phase extraction with individual SPE columns (Sample Clean Up Columns). The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using 96 SPE well plates (72072/96, 72072/480).

Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.