MassChrom® Steroids in Serum/Plasma with 96-SPE-Well-Plate - LC-MS/MS

Order No.: 72072/96 & 72072/480, for 96 / 480 tests
Parameters:
11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Optimised and extended method
15 Steroids – 2 panels – 1 analytical column
With 96 SPE Well Plate

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Grouped product items
Product Name Qty
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
Order No.: 72072/96
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
Order No.: 72072/480

Steroid Panel 1

Steroid Panel 2

Steroid Panel 1

Aldosterone

Corticosterone

Cortisol

11-Deoxycortisol

21-Deoxycortisol

Cortisone

Steroid Panel 2

11-Deoxycorticosterone

Androstenedione

Dehydroepiandrosterone (DHEA)

Dehydroepiandrosterone sulfate (DHEA-S)

Estradiol

Progesterone

17-OH-Progesterone

Testosterone

Dihydrotestosterone (DHT)

Clinical relevance

Steroid hormones are structurally derived from cholesterol and play a role in a wide range of physiological processes, including carbohydrate metabolism, fat and protein metabolism as well as in stress response. They are secreted by the adrenal cortex, testes, ovaries, and during pregnancy by the placenta. They are transported through the bloodstream to the cells of various organs where they influence and regulate the organs’ physiological functions.

The determination of hormones is important for the diagnosis of a number of diseases such as adrenogenital syndrome, hyperaldosteronism, or tumours. By using LC-MS/MS, not only high sensitivity and specificity can be achieved, but also the efficient measurement of multiple analytes in a single run.

 

Product advantages

• 15 steroids – 2 panels – 1 analytical column
• One sample preparation for all parameters
• Each parameter safeguarded by its own internal standard

 

Assay characteristics

This Chromsystems assay allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient SPE sample clean up procedure with 96 SPE well plates. The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using Sample Clean Up Columns (72072/C).

Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96 / 480
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

Aldosterone: 0.014 µg/l – 6.00 µg/l
Cortisol: 1.52 µg/l – 521 µg/l
Cortisone: 0.148 µg/l – 51.8 µg/l
Progesterone: 0.030 µg/l – 25.0 µg/l
Testosterone: 0.005 µg/l – 24.0 µg/l

For the values of all analytes please refer to the instruction manual.

Different systems might show different performance data.

Specimen Serum/Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Put 96 SPE Well Plate onto Waste Plate.
  • Pipette 0.8 ml Equilibration Reagent 1 into each well of the 96 SPE Well Plate, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each well, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Place plate onto the Collection Plate.
  • Add 500 μl Elution Buffer and centrifuge.
  • Evaporate eluates in the Collection Plate (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Shake Collection Plate, seal with adhesive seal and inject 5–50 μl of the eluate. 
Run Time Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min
Injection Volume 5-50 µl
Column Temperature Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C
Gradient binary
Ionisation ESI negative and positive (ESI switch)
MS/MS Mode MRM
Parameters 11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Steroid Panel 1

Steroid Panel 2

Steroid Panel 1

Aldosterone

Corticosterone

Cortisol

11-Deoxycortisol

21-Deoxycortisol

Cortisone

Steroid Panel 2

11-Deoxycorticosterone

Androstenedione

Dehydroepiandrosterone (DHEA)

Dehydroepiandrosterone sulfate (DHEA-S)

Estradiol

Progesterone

17-OH-Progesterone

Testosterone

Dihydrotestosterone (DHT)

Clinical relevance

Steroid hormones are structurally derived from cholesterol and play a role in a wide range of physiological processes, including carbohydrate metabolism, fat and protein metabolism as well as in stress response. They are secreted by the adrenal cortex, testes, ovaries, and during pregnancy by the placenta. They are transported through the bloodstream to the cells of various organs where they influence and regulate the organs’ physiological functions.

The determination of hormones is important for the diagnosis of a number of diseases such as adrenogenital syndrome, hyperaldosteronism, or tumours. By using LC-MS/MS, not only high sensitivity and specificity can be achieved, but also the efficient measurement of multiple analytes in a single run.

 

Product advantages

• 15 steroids – 2 panels – 1 analytical column
• One sample preparation for all parameters
• Each parameter safeguarded by its own internal standard

 

Assay characteristics

This Chromsystems assay allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient SPE sample clean up procedure with 96 SPE well plates. The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using Sample Clean Up Columns (72072/C).

Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96 / 480
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

Aldosterone: 0.014 µg/l – 6.00 µg/l
Cortisol: 1.52 µg/l – 521 µg/l
Cortisone: 0.148 µg/l – 51.8 µg/l
Progesterone: 0.030 µg/l – 25.0 µg/l
Testosterone: 0.005 µg/l – 24.0 µg/l

For the values of all analytes please refer to the instruction manual.

Different systems might show different performance data.

Specimen Serum/Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Put 96 SPE Well Plate onto Waste Plate.
  • Pipette 0.8 ml Equilibration Reagent 1 into each well of the 96 SPE Well Plate, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each well, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Place plate onto the Collection Plate.
  • Add 500 μl Elution Buffer and centrifuge.
  • Evaporate eluates in the Collection Plate (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Shake Collection Plate, seal with adhesive seal and inject 5–50 μl of the eluate. 
Run Time Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min
Injection Volume 5-50 µl
Column Temperature Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C
Gradient binary
Ionisation ESI negative and positive (ESI switch)
MS/MS Mode MRM
Parameters 11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.