MassChrom® Steroids in Serum/Plasma with 96-SPE-Well-Plate - LC-MS/MS
Optimised and extended method
15 Steroids – 2 panels – 1 analytical column
With 96 SPE Well Plate
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
Steroid Panel 1
Steroid Panel 2
Steroid Panel 1
Aldosterone
Corticosterone
Cortisol
11-Deoxycortisol
21-Deoxycortisol
Cortisone
Steroid Panel 2
11-Deoxycorticosterone
Androstenedione
Dehydroepiandrosterone (DHEA)
Dehydroepiandrosterone sulfate (DHEA-S)
Estradiol
Progesterone
17-OH-Progesterone
Testosterone
Dihydrotestosterone (DHT)
Clinical relevance
Steroid hormones are structurally derived from cholesterol and play a role in a wide range of physiological processes, including carbohydrate metabolism, fat and protein metabolism as well as in stress response. They are secreted by the adrenal cortex, testes, ovaries, and during pregnancy by the placenta. They are transported through the bloodstream to the cells of various organs where they influence and regulate the organs’ physiological functions.
The determination of hormones is important for the diagnosis of a number of diseases such as adrenogenital syndrome, hyperaldosteronism, or tumours. By using LC-MS/MS, not only high sensitivity and specificity can be achieved, but also the efficient measurement of multiple analytes in a single run.
Product advantages
• 15 steroids – 2 panels – 1 analytical column
• One sample preparation for all parameters
• Each parameter safeguarded by its own internal standard
Assay characteristics
This Chromsystems assay allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient SPE sample clean up procedure with 96 SPE well plates. The method does not require a high-end LC-MS/MS system to be carried out.
Alternatively, there is also a method available using Sample Clean Up Columns (72072/C).
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 / 480 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | Aldosterone: 0.014 µg/l – 6.00 µg/l For the values of all analytes please refer to the instruction manual. Different systems might show different performance data. |
| Specimen | Serum/Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min |
| Injection Volume | 5-50 µl |
| Column Temperature | Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C |
| Gradient | binary |
| Ionisation | ESI negative and positive (ESI switch) |
| MS/MS Mode | MRM |
| Parameters | 11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone |
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Pierceable adhesive seals for 96 SPE Well PlatesOrder no.: 72059MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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Analytical Column Steroids in Serum/Plasma - LC-MS/MSOrder no.: 72110MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 1Order no.: 72038MassChrom® Steroids in Serum/Plasma - LC-MS/MS -
6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 2Order no.: 72039MassChrom® Steroids in Serum/Plasma - LC-MS/MS -
MassCheck® Steroid Panel 1 Serum ControlsOrder no.: 0341/0342/0343MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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MassCheck® Steroid Panel 2 Serum ControlsOrder no.: 0345/0346/0347MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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System Check Solution MassChrom® Steroid Panel 1Order no.: 72088MassChrom® Steroids in Serum/Plasma - LC-MS/MS
MassChrom® Cortisol, Cortisone in Saliva - LC-MS/MS -
System Check Solution MassChrom® Steroid Panel 2Order no.: 72099MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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Tuning Mix MassChrom® Steroid Panel 1Order no.: 72019MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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Tuning Mix MassChrom® Steroid Panel 2Order no.: 72020MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 1Order no.: 72038MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 2Order no.: 72039MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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MassCheck® Steroid Panel 2 Serum ControlsOrder no.: 0345/0346/0347
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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MassCheck® Steroid Panel 1 Serum ControlsOrder no.: 0341/0342/0343
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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Analytical Column Steroids in Serum/Plasma - LC-MS/MSOrder no.: 72110
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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Sample Concentrator for 96 Well PlatesOrder no.: 42720Fast evaporation of solvents
Light and compact unit for easy use in fume cupboards
High-quality stainless-steel needles
Accurate height control
Chromsystems-adapted blocks available for specific assay requirements
Steroid Panel 1
Steroid Panel 2
Steroid Panel 1
Aldosterone
Corticosterone
Cortisol
11-Deoxycortisol
21-Deoxycortisol
Cortisone
Steroid Panel 2
11-Deoxycorticosterone
Androstenedione
Dehydroepiandrosterone (DHEA)
Dehydroepiandrosterone sulfate (DHEA-S)
Estradiol
Progesterone
17-OH-Progesterone
Testosterone
Dihydrotestosterone (DHT)
Clinical relevance
Steroid hormones are structurally derived from cholesterol and play a role in a wide range of physiological processes, including carbohydrate metabolism, fat and protein metabolism as well as in stress response. They are secreted by the adrenal cortex, testes, ovaries, and during pregnancy by the placenta. They are transported through the bloodstream to the cells of various organs where they influence and regulate the organs’ physiological functions.
The determination of hormones is important for the diagnosis of a number of diseases such as adrenogenital syndrome, hyperaldosteronism, or tumours. By using LC-MS/MS, not only high sensitivity and specificity can be achieved, but also the efficient measurement of multiple analytes in a single run.
Product advantages
• 15 steroids – 2 panels – 1 analytical column
• One sample preparation for all parameters
• Each parameter safeguarded by its own internal standard
Assay characteristics
This Chromsystems assay allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient SPE sample clean up procedure with 96 SPE well plates. The method does not require a high-end LC-MS/MS system to be carried out.
Alternatively, there is also a method available using Sample Clean Up Columns (72072/C).
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 / 480 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | Aldosterone: 0.014 µg/l – 6.00 µg/l For the values of all analytes please refer to the instruction manual. Different systems might show different performance data. |
| Specimen | Serum/Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min |
| Injection Volume | 5-50 µl |
| Column Temperature | Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C |
| Gradient | binary |
| Ionisation | ESI negative and positive (ESI switch) |
| MS/MS Mode | MRM |
| Parameters | 11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone |
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Pierceable adhesive seals for 96 SPE Well PlatesOrder no.: 72059
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
-
Analytical Column Steroids in Serum/Plasma - LC-MS/MSOrder no.: 72110
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 1Order no.: 72038MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 2Order no.: 72039MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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MassCheck® Steroid Panel 1 Serum ControlsOrder no.: 0341/0342/0343
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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MassCheck® Steroid Panel 2 Serum ControlsOrder no.: 0345/0346/0347
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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System Check Solution MassChrom® Steroid Panel 1Order no.: 72088
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
MassChrom® Cortisol, Cortisone in Saliva - LC-MS/MS -
System Check Solution MassChrom® Steroid Panel 2Order no.: 72099
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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Tuning Mix MassChrom® Steroid Panel 1Order no.: 72019
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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Tuning Mix MassChrom® Steroid Panel 2Order no.: 72020
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 1Order no.: 72038MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set Steroid Panel 2Order no.: 72039MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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MassCheck® Steroid Panel 2 Serum ControlsOrder no.: 0345/0346/0347
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
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MassCheck® Steroid Panel 1 Serum ControlsOrder no.: 0341/0342/0343
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
-
Analytical Column Steroids in Serum/Plasma - LC-MS/MSOrder no.: 72110
MassChrom® Steroids in Serum/Plasma - LC-MS/MS
-
Sample Concentrator for 96 Well PlatesOrder no.: 42720
Fast evaporation of solvents
Light and compact unit for easy use in fume cupboards
High-quality stainless-steel needles
Accurate height control
Chromsystems-adapted blocks available for specific assay requirements