Vitamins A and E
This assay enables the simultaneous quantitative determination of vitamin A (retinol) and vitamin E (α-tocopherol) in human serum or plasma samples by HPLC. The fast and easy sample preparation can be performed with reaction vials or 96 deep well plates. The analytes are released from their transport protein or lipoproteins by precipitation. Subsequently, the substances are chromatographically separated in one isocratic HPLC run and quantified using a UV detector.
- Assay validated according to IVDR
- Short run time of less than 3 minutes
- Optimised precipitation
- No cooling of eluates – no cooled autosampler necessary
- No precolumn required
- No UHPLC needed
![Drugs of Abuse Drugs of Abuse](https://chromsystems.com/media/.renditions/images/pages/Vitamin-AE-Header_840x600Px.jpg)
![Drugs of Abuse Drugs of Abuse](https://chromsystems.com/media/.renditions/images/pages/Vitamin-AE-Header_840x600Px.jpg)
Clinical Relevance
The assay is intended to be used for determination of vitamin A and vitamin E levels where indicated: for screening in patients with suspected vitamin A and/or E deficiency or excess and for monitoring of patients under vitamin A and/or E supplementation.
Two Types of Kits Available
Reagent kit
Mobile phase, calibrator and sample preparation reagents included
Basic kit
Only sample preparation reagents included
Vitamins A and E in Serum/Plasma
Order no. | Type of kit |
Characteristics | Method | Number |
34400 | Reagent kit | With reaction vials | HPLC | 200 tests |
34400-DWP | Reagent kit | With 96 deep well plates | HPLC | 200 tests |
34400-BK | Basic kit | With reaction vials | HPLC | 200 sample preparations |
34400-DWP-BK | Basic kit | With 96 deep well plates | HPLC | 200 sample preparations |
Easy Sample Preparation
Here shown with reaction vials.
The assay is also available with 96 deep well plates.
![Vitamins A and E - Sample Preparation Vitamins A and E - Sample Preparation](https://chromsystems.com/media/.renditions/images/pages/Vita-AE-Sample-Prep-1000Px.jpg)
![Vitamins A and E - Sample Preparation Vitamins A and E - Sample Preparation](https://chromsystems.com/media/.renditions/images/pages/Vita-AE-Sample-Prep-1000Px.jpg)
HPLC Column Temperature
Column temperature: 35 °C
![](https://chromsystems.com/media/.renditions/images/pages/Vita-AE-Temp-1500Px_1.jpg)
![](https://chromsystems.com/media/.renditions/images/pages/Vita-AE-Temp-1500Px_1.jpg)
Column temperature: ambient
![Vitamins A and E - Column Temperature Vitamins A and E - Column Temperature](https://chromsystems.com/media/.renditions/images/pages/Vita-AE-Temp-1500Px-2.jpg)
![Vitamins A and E - Column Temperature Vitamins A and E - Column Temperature](https://chromsystems.com/media/.renditions/images/pages/Vita-AE-Temp-1500Px-2.jpg)
Technical Data
Specifications | Vitamins A and E |
Lower and Upper Limit of Quantitation |
Vitamin A (retinol): serum: 0.02 – 5.0 mg/l, plasma: 0.01 – 5.0 mg/l |
Specimens | Serum/plasma |
Run Time | Depending on column temperature: 2.9 min (35 °C) or 3.7 min (ambient |
Injection Volume | 5 – 20 μl |
Flow Rate | 0.8 ml/min |
Wavelength | 325 nm after 1.15 – 1.4 min switch to 295 nm |
Download Brochure
![Drugs of Abuse Brochure Drugs of Abuse Brochure Chromsystems](https://chromsystems.com/media/.renditions/images/print-titel/CS-EN_Vitamin-AE-34400_Title_300Px.jpg)
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