Vitamins A and E

This assay enables the simultaneous quantitative determination of vitamin A (retinol) and vitamin E (α-tocopherol) in human serum or plasma samples by HPLC. The fast and easy sample preparation can be performed with reaction vials or 96 deep well plates. The analytes are released from their transport protein or lipoproteins by precipitation. Subsequently, the substances are chromatographically separated in one isocratic HPLC run and quantified using a UV detector.

• Assay validated according to IVDR
• Short run time of less than 3 minutes
• Optimised precipitation
• No cooling of eluates – no cooled autosampler necessary
• No precolumn required
• No UHPLC needed

The assay is intended to be used for determination of vitamin A and vitamin E levels where indicated: for screening in patients with suspected vitamin A and/or E deficiency or excess and for monitoring of patients under vitamin A and/or E supplementation.

Mobile phase, calibrator and sample preparation reagents included

Only sample preparation reagents included

Here shown with reaction vials.

The assay is also available with 96 deep well plates.

Column temperature: 35 °C

Column temperature: ambient