Vitamin B1/B6 in Whole Blood, fully automated analysis - HPLC
Significantly reduced hands-on time for sample preparation
Accurate and fast results for both vitamins
Each parameter safeguarded by a specific internal standard
Vitamin B1 (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in carbohydrate metabolism and for pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of the TPP concentration is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account.
Vitamin B6 comprises pyridoxine, pyridoxamine and pyridoxal, as well as their phosphate derivatives. It is ingested with food and transferred via several enzymatic conversions into its active form, pyridoxal-5’-phosphate (PLP). PLP is involved in nearly one hundred different enzymatic reactions, among other things, as co-factor in amino acid metabolism, and in the formation of haemoglobin or neurotransmitters in the brain.
- Automated sample preparation for minimised workload in the laboratory
- Reliable and reproducible results with a specific internal standard for each parameter
This HPLC kit facilitates the combined analysis of vitamin B1 in whole blood and vitamin B6 in whole blood or plasma. Sample preparation is automated in 96 well filter plates for maximum output and minimal workload. The internal standard for each of the two parameters and special quality controls ensure precise and reliable quantification.
Chromsystems also offers two kits that feature manual determination using HPLC: a standard version as well as a kit for simplified sample preparation with pre-mixed tubes.
Alternatively, an automated method using UHPLC is also available.
|Method of Analysis||HPLC|
|Number of Tests||960|
|Limit of quantification||
PLP 2.0 μg/l
PLP 500 μg/l
CV < 3 %
CV < 5 %
|Analysis Time||9 min|
|Sample Stability||Samples are stable up to 1 week at +2 to +8 °C and up to 2 weeks at below -18 °C. Protect from light.|
|Injection Volume||25–50 μl|
|Flow rate||1.5–2.3 ml/min|
|Column temperature||ambient (~ 25°C)|
Start EX 320 nm, EM 415 nm,
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Parameter||Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate)|