Amino Acid Analysis – Now Also in Urine
Quantification of 52 amino acids and creatinine with LC-MS/MS in one run
MassChrom® Amino Acid Analysis in Urine is a new assay that can be used for a number of applications, such as the determination of renal aminoaciduria, and can be ideally combined with the plasma assay without needing to change the chromatographic set-up.
The assay is IVDR-ready, which means it has been developed and manufactured according to the new regulation (IVDR).
Parameter Menu
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Acetyltyrosine Adenosylhomocysteine Alanine β-Alanine α-Aminoadipic acid α-Aminobutyric acid β-Aminoisobutyric acid γ-Aminobutyric acid Anserine Arginine Argininosuccinic acid Asparagine Aspartic acid Carnosine |
Citrulline Homocitrulline Cystathionine Cystine Cysteine S-sulfate Homocystine Ethanolamine Phosphoethanolamine Glutamic acid Glutamine Glycine Histidine 1-Methylhistidine 3-Methylhistidine |
Kynurenine Hydroxykynurenine Leucine Allo-Isoleucine Isoleucine Lysine Hydroxylysine Methionine Ornithine Oxoproline / Pyroglutamic acid Phenylacetylglutamine Phenylalanine Phosphoserine Pipecolic acid |
Proline 4-Hydroxyproline Saccharopine Sarcosine Serine Taurine Threonine Tryptophan Tyrosine Valine Creatinine * |
*The assay allows the determination of the creatinine concentration in urine within the same run.
Reliable and Fast: MassChrom® Amino Acid Analysis in Urine by LC-MS/MS
- Creatinine normalisation within the same run
- Fast sample preparation without derivatisation
- Short run times
- Excellent separation of all isobaric compounds
- Each analyte with its own internal standard
- One chromatographic platform for plasma/serum and urine
Chromatogram MassChrom® Amino Acid Analysis in Urine
Creatinine Normalisation in the Same Run
For the normalisation of urine samples, creatinine is usually determined photometrically.
Depending on the laboratory, the urine is then diluted to 1 mmol/l creatinine before determining the amino acids. This is an error-prone and time-consuming step. However, MassChrom® AAA is the first assay on the market that allows creatinine determination together with the amino acids in the same LC-MS/MS run. This is not only faster and less error-prone, but it also eliminates the need for diluting the urine samples. A comparison of the methods shows a very good agreement of the creatinine values between the photometric method and our MassChrom® assay.
Complete Solution for the Amino Acid Analysis (AAA) in Plasma/Serum and Urine
MassChrom® Amino Acid Analysis in Urine can be ideally combined with our plasma assay. Both kits use the same chromatographic set-up that includes mobile phases, rinsing solution and analytical column. Laboratories can therefore determine these matrices in a single LC-MS/MS batch according to daily requirements and without changes to the instrument. Existing users of the plasma/serum assay extend their analysis by ordering the urine set and appropriate calibrators and controls.
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