MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns - LC-MS/MS

Order No.: 72072/C/RUO, for 96 tests
Parameters:
11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Optimised and extended method
15 Steroids – 2 panels – 1 analytical column
With Sample Clean Up Columns

Steroid Panel 1

Steroid Panel 2

Steroid Panel 1

Aldosterone

Corticosterone

Cortisol

11-Deoxycortisol

21-Deoxycortisol

Cortisone

Steroid Panel 2

11-Deoxycorticosterone

Androstenedione

Dehydroepiandrosterone (DHEA)

Dehydroepiandrosterone sulfate (DHEA-S)

Estradiol

Progesterone

17-OH-Progesterone

Testosterone

Dihydrotestosterone (DHT)

This MassChrom® Steroid kit allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient solid phase extraction with individual SPE columns (Sample Clean Up Columns). The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantitation of the steroids. The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using 96 SPE well plates (72072/96/RUO, 72072/480/RUO).

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Put Sample Clean Up Columns into tubes for sample preparation.
  • Pipette 0.8 ml Equilibration Reagent 1 into each Sample Clean Up Column, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl research sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each Sample Clean Up Column, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Put autosampler vials into tubes for sample preparation, then put Sample Clean Up Columns on top.
  • Add 1 ml Elution Buffer and centrifuge.
  • Evaporate eluates in the autosampler vials (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Close autosampler vials, mix briefly (vortex) and inject 5–50 μl of the eluate.  
Run Time Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min
Injection Volume 5-50 µl
Column Temperature Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C
Gradient binary
Ionisation ESI negative and positive (ESI switch)
MS/MS Mode MRM
Parameters 11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:

Steroid Panel 1

Steroid Panel 2

Steroid Panel 1

Aldosterone

Corticosterone

Cortisol

11-Deoxycortisol

21-Deoxycortisol

Cortisone

Steroid Panel 2

11-Deoxycorticosterone

Androstenedione

Dehydroepiandrosterone (DHEA)

Dehydroepiandrosterone sulfate (DHEA-S)

Estradiol

Progesterone

17-OH-Progesterone

Testosterone

Dihydrotestosterone (DHT)

This MassChrom® Steroid kit allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient solid phase extraction with individual SPE columns (Sample Clean Up Columns). The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantitation of the steroids. The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using 96 SPE well plates (72072/96/RUO, 72072/480/RUO).

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Put Sample Clean Up Columns into tubes for sample preparation.
  • Pipette 0.8 ml Equilibration Reagent 1 into each Sample Clean Up Column, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl research sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each Sample Clean Up Column, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Put autosampler vials into tubes for sample preparation, then put Sample Clean Up Columns on top.
  • Add 1 ml Elution Buffer and centrifuge.
  • Evaporate eluates in the autosampler vials (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Close autosampler vials, mix briefly (vortex) and inject 5–50 μl of the eluate.  
Run Time Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min
Injection Volume 5-50 µl
Column Temperature Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C
Gradient binary
Ionisation ESI negative and positive (ESI switch)
MS/MS Mode MRM
Parameters 11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method: