MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns - LC-MS/MS

Order No.: 72072/C/RUO, for 96 tests
Parameters:
11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Optimised and extended method
15 Steroids – 2 panels – 1 analytical column
With Sample Clean Up Columns

Steroid Panel 1

Steroid Panel 2

Steroid Panel 1

Aldosterone

Corticosterone

Cortisol

11-Deoxycortisol

21-Deoxycortisol

Cortisone

Steroid Panel 2

11-Deoxycorticosterone

Androstenedione

Dehydroepiandrosterone (DHEA)

Dehydroepiandrosterone sulfate (DHEA-S)

Estradiol

Progesterone

17-OH-Progesterone

Testosterone

Dihydrotestosterone (DHT)

This MassChrom® Steroid kit allows the quantitative determination of 15 steroids in serum/plasma by LC-MS/MS. Sample preparation is the same for both steroid panels using an optimised and efficient solid phase extraction with individual SPE columns (Sample Clean Up Columns). The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantitation of the steroids. The method does not require a high-end LC-MS/MS system to be carried out.

Alternatively, there is also a method available using 96 SPE well plates (72072/96/RUO, 72072/480/RUO).

More Information
Number of Tests 96
Analysis Time Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min
Sample Preparation
  • Put Sample Clean Up Columns into tubes for sample preparation.
  • Pipette 0.8 ml Equilibration Reagent 1 into each Sample Clean Up Column, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl research sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each Sample Clean Up Column, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Put autosampler vials into tubes for sample preparation, then put Sample Clean Up Columns on top.
  • Add 1 ml Elution Buffer and centrifuge.
  • Evaporate eluates in the autosampler vials (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Close autosampler vials, mix briefly (vortex) and inject 5–50 μl of the eluate.  
Sample Stability Stability of research samples depends on the specific steroid. Further information can be obtained from the instruction manual.
Specimen Serum/Plasma
Injection Volume 5-50 µl
Column temperature Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C
Gradient binary
Ionisation ESI negative and positive (ESI switch)
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-Progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
  1. Mobile Phase A
    Mobile Phase A
    Order no.: 72011/RUO

    MassChrom® Steroids in Serum/Plasma (72072/RUO) - LC-MS/MS

    Details
  2. Mobile Phase B
    Mobile Phase B
    Order no.: 72002/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  3. 72033 Elution Buffer for Steroids in Serum Plasma
    Elution Buffer
    Order no.: 72033/RUO

    MassChrom® Steroids in Serum/Plasma (72072/RUO) - LC-MS/MS

    Details
  4. 72044 internal standard mix steroids serum plasma
    Internal Standard Mix
    Order no.: 72044/RUO

    MassChrom® Steroids in Serum/Plasma (72072/RUO) - LC-MS/MS

    Details
  5. Extraction Buffer
    Extraction Buffer
    Order no.: 72005/RUO

    MassChrom® Steroids in Serum/Plasma

    Details
  6. Reconstitution Buffer
    Reconstitution Buffer
    Order no.: 72006/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  7. Equilibration Buffer 1 for Steroids in Serum/Plasma
    Equilibration Reagent 1
    Order no.: 72077/RUO

    MassChrom® Steroids in Serum/Plasma (72072/RUO) - LC-MS/MS

    Details
  8. Equilibration Reagent 2
    Equilibration Reagent 2
    Order no.: 72008/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  9. Rinsing Solution
    Rinsing Solution
    Order no.: 72009/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  10. Wash Buffer
    Wash Buffer
    Order no.: 72012/RUO

    MassChrom® Steroids in Serum/Plasma (72072/RUO) - LC-MS/MS

    Details
  11. 72055 Sample Clean Up Columns
    Sample Clean Up Columns
    Order no.: 72055

    MassChrom® Steroids in Serum/Plasma (72072/C/RUO) - LC-MS/MS

    Details
  1. 0345 0346 0347 LCMS steroid panel2 controls serum plasma
    Details
  2. 0341 0342 0343 MassCheck Steroids Serum Controls
    MassCheck® Steroid Panel 1 Serum Controls
    Order no.: 0341/0342/0343

    Order no.: 0341/0342/0343

    Details
  1. 72110 Analytical Column for Steroids in Serum/Plasma
    Analytical Column Steroids in Serum/Plasma - LC-MS/MS
    Order no.: 72110

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  2. Tuning Mix MassChrom® Steroid Panel 1
    Tuning Mix MassChrom® Steroid Panel 1
    Order no.: 72019/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  3. Tuning Mix MassChrom® Steroid Panel 2
    Tuning Mix MassChrom® Steroid Panel 2
    Order no.: 72020/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  4. Tubes for sample prep
    Tubes for Sample Preparation, 13 ml
    Order no.: 33007

    PP, 95 x 16.8 mm

    Details
  5. System Check Solution MassChrom® Steroid Panel 1
    System Check Solution MassChrom® Steroid Panel 1
    Order no.: 72088/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details
  6. System Check Solution MassChrom® Steroid Panel 2
    System Check Solution MassChrom® Steroid Panel 2
    Order no.: 72099/RUO

    MassChrom® Steroids in Serum/Plasma - LC-MS/MS

    Details