Automated Vitamins A and E in Serum/Plasma - HPLC
Automated sample preparation with 96 Well Filter Plates
96 samples in less than 30 minutes
Works on a range of liquid handling devices
Vitamin A
Vitamin E
Clinical relevance
Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.
Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.
Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.
However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.
Product advantages
- Automated sample preparation:
96 samples in less than 30 minutes
This HPLC kit allows for the automated analysis of vitamins A and E in plasma/serum. The automated preparation of 96 samples can be completed in less than 30 minutes – ideal for resource-saving processing of larger numbers of samples.
Chromsystems also offers another automated method using UHPLC.
| Number of Tests | 5 x 96 |
|---|---|
| Limit of quantification | Vitamin A: 0.02 mg/l Vitamin E: 0.5 mg/l |
| Linearity | Vitamin A: 0.02-4.5 mg/l Vitamin E: 0.5-38 mg/l |
| Recovery | Vitamin A > 94 %; Vitamin E > 90 % |
| Intraassay | CV < 5 % |
| Interassay | CV < 5 % |
| Analysis Time | 9 min |
| Sample Preparation |
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| Sample Stability | Samples are stable up to 48 hours stored light protected in a tightly closable glass container at +2 to +8°C. For longer storage periods (up to 3 weeks) keep samples frozen below -18 °C. |
| Specimen | Serum/Plasma |
| Injection Volume | 50 µl |
| Column temperature | ambient (~ 25°C) |
| Wavelength | 325 nm, after 3.5 min switch to 295 nm |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Method of Analysis | HPLC |
| Parameter | Vitamin A, Vitamin E |
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Pierceable Heat Seals for 96 Well PlatesOrder no.: 34760Automated Vitamins A and E in Serum/Plasma - HPLC/UHPLC
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Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
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HPLC Column Vitamins A and E in Serum/PlasmaOrder no.: 34100Vitamins A and E in Serum/Plasma - HPLC
