Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate
7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480)
Clinical relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.
Product advantages
- 7 analytes in one single run
- Efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).
This Sample Prep Set encompasses all required components for the sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline (norepinephrine): 4.00 µg/l – 1600 µg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 4 min |
| Injection Volume | ≤ 10 µl |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline (Norepinephrine), Normetanephrine, Serotonin |
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Internal Standard Mix - 5 x 5 mlOrder no.: 80604/5MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Pierceable adhesive seals for 96 Well PlatesOrder no.: 80059MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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MassChrom® Biogenic Amines/Metabolites in Urine – Starter Set Chromatographic PlatformOrder no.: 80000Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
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Tuning Mix MassChrom® Biogenic Amines/MetabolitesOrder no.: 80615MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
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MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480)
Clinical relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these biogenic amines are of clinical importance.
Product advantages
- 7 analytes in one single run
- Efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).
This Sample Prep Set encompasses all required components for the sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | Noradrenaline (norepinephrine): 4.00 µg/l – 1600 µg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 4 min |
| Injection Volume | ≤ 10 µl |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline (Norepinephrine), Normetanephrine, Serotonin |
-
Internal Standard Mix - 5 x 5 mlOrder no.: 80604/5
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
-
-
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 80059
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
MassChrom® Biogenic Amines/Metabolites in Urine – Starter Set Chromatographic PlatformOrder no.: 80000
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
-
Tuning Mix MassChrom® Biogenic Amines/MetabolitesOrder no.: 80615
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS