Automated Vitamins A and E in Serum/Plasma - UHPLC

Order No.: 34900/F, for 10 x 96 tests
Parameters:
Vitamin A, Vitamin E

Automated sample preparation with 96 Well Filter Plates
96 samples in less than 30 minutes
Works on a range of liquid handling devices

Vitamin A

Vitamin E

Clinical relevance

Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.

Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.

Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.

However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.

 

Product advantages

  • Automated sample preparation:
    96 samples in less than 30 minutes

 

This UHPLC kit allows for the automated analysis of vitamins A and E in plasma/serum. The automated preparation of 96 samples can be completed in less than 30 minutes – ideal for resource-saving processing of larger numbers of samples. The UHPLC determination is performed in less than 4 minutes.

Chromsystems also offers another automated method using HPLC.

 

Kit content for 10 x 96 analyses:

Mobile Phase, 2 x 500 ml
Precipitation Reagent I, 4 x 2.5 ml
Internal Standard, 2 x 100 ml
Vitamins A and E Serum Calibration Standard (lyoph.), 5 x 1 ml
96 Well Filter Plates, 2 x 5 pcs.
96 Well Collection Plates, 2 x 5 pcs.
Pierceable Heat Seals for 96 Well Plates, 2 x 6 pcs.
UHPLC Column, 1 pc.
UHPLC Precolumn cartridge, 3 pcs.

More Information
Number of Tests 10 x 96
Limit of quantification

Vitamin A: 0.02 mg/l
Vitamin E: 0.4 mg/l

Linearity Vitamin A: 0.02-4.5 mg/l Vitamin E: 0.4-38 mg/l
Recovery Vitamin A > 94 %; Vitamin E > 90 %
Intraassay

Vitamin A: CV < 5 %
Vitamin E: CV < 5 %

Interassay

Vitamin A: CV < 5 %
Vitamin E: CV < 5 %

Analysis Time 3.5 min
Sample Preparation
  • Place 200 μl Internal Standard to the 96 well filter plate.
  • Add 50 μl sample/calibrator/control.
  • Add 10 μl Precipitation Reagent I.
  • Agitate the 96 well filter plate for 1 min.
  • Separate precipitate by centrifugation or suction (2 min at 1500 x g or 200 mbar, resp.).
  • Collect eluate in a collection plate.
  • Seal the collection plate with a heat seal and transfer to autosampler.
Sample Stability Samples are stable up to 48 hours stored light protected in a tightly closable glass container at +2 to +8°C. For longer storage periods (up to 3 weeks) keep samples frozen below -18 °C.
Specimen Serum/Plasma
Injection Volume 5 µl
Column temperature approx. 35°C
Wavelength 325 nm, after 1.3 min switch to 295 nm
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis UHPLC
Parameter Vitamin A, Vitamin E
  1. 34004 vitamin A vitamin E serum plasma calibration standard
    Vitamins A and E Serum Calibration Standard
    Order no.: 34004
    Vitamins A and E in Serum/Plasma- HPLC/UHPLC
    Details
  2. 34006 vitamin A vitamin E serum plasma precipitation reagent
    Precipitation Reagent I
    Order no.: 34006
    Vitamins A and E in Serum/Plasma - HPLC/UHPLC
    Details
  3. 34708 vitamin A vitamin E serum plasma internal standard
    Internal Standard
    Order no.: 34708
    Automated Vitamins A and E in Serum/Plasma - HPLC/UHPLC
    Details
  4. 34757 34758 34760 vitamin A vitamin E serum plasma well filter plate well collection plates seals
    96 Well Filter Plates
    Order no.: 34757
    Automated Vitamin A and E in Serum/Plasma - HPLC/UHPLC
    Details
  5. 34757 34758 34760 vitamin A vitamin E serum plasma well filter plate well collection plates seals
    96 Well Collection Plates
    Order no.: 34758
    Automated Vitamins A and E in Serum/Plasma - HPLC/UHPLC
    Details
  6. 34757 34758 34760 vitamin A vitamin E serum plasma well filter plate well collection plates seals
    Pierceable Heat Seals for 96 Well Plates
    Order no.: 34760
    Automated Vitamins A and E in Serum/Plasma - HPLC/UHPLC
    Details
  1. 34004 vitamin A vitamin E serum plasma calibration standard
    Vitamins A and E Serum Calibration Standard
    Order no.: 34004
    Vitamins A and E in Serum/Plasma- HPLC/UHPLC
    Details
  1. 0032 0036 0037 HPLC vitamin A vitamin E serum controls
    Vitamins A and E Serum Controls
    Order no.: 0032/0036/0037
    Details
  1. UHPLC Precolumn cartridge holder
    UHPLC Precolumn cartridge holder
    Order no.: 19001
    Vitamins A and E - UHPLC
    Details
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