VMA, HVA, 5-HIAA in Urine - HPLC
No pH-adjustment necessary
Suitable for automated sample preparation
2 Internal standards available
Neuroblastoma is a neoplastic disease that is among the most common malignant cancers occurring during childhood. It is a neuroendocrine tumour resulting in higher excretion of the catecholamine metabolites vanillylmandelic acid (VMA) and homovanillic acid (HVA) in the urine. Therefore verification of these parameters is critical in the diagnosis of neuroblastoma.
In contrast, increased concentrations of hydroxyindoleacetic acid (5-HIAA) detected in the urine are an indication of a carcinoid tumour. The malignant reproduction of enterochromaffin cells in the gastrointestinal tract leads to increased production of the tissue hormone serotonin, whose primary metabolite is 5-HIAA.
- Simple and reliable sample preparation
- Two internal standards if desired
- Automated sample preparation for Gilson® ASPECTM is available
This kit enables routine HPLC determination of VMA, HVA and 5-HIAA in urine. Simple and safe operation guarantees reliable and reproducible results. An additional, optional internal standard (HICA) is also available for especially high precision in the determination of 5-HIAA.
Mobile Phase, 1000 ml
VMA, HVA, 5-HIAA Calibration Standard (lyoph.), 5 x 1 ml
Internal Standard (iso-VMA), 100 ml
Wash Buffer I, 300 ml
Wash Buffer II, 300 ml
Elution Buffer, 200 ml
Finisher, 10 ml
Sample Clean Up Columns, 50 pcs.
|Number of Tests||100|
|Limit of quantification||
VMA, HVA, 5-HIAA: 0.5 mg/l
VMA: up to 78 mg/l
|Recovery||VMA: 70 %; HVA: 82 %; 5-HIAA: 66 %|
|Intraassay||CV < 4 %|
|Interassay||CV < 5 %|
|Analysis Time||< 18 min|
|Sample Stability||VMA, HVA: at +2 to +8 °C at least 5 days. 5-HIAA: at +2 to +8 °C in the dark max. 3 days. Aliquot and freeze below -18 °C for longer storage.|
|Specimen||24 h urine; spontaneous urine (refer the data to urine creatinine)|
Determination of VMA/HVA
Collect urine in 10 ml HCl 25 %, pH < 4
Determination of 5-HIAA
Collect urine in 10 ml glacial acetic acid, pH 4 - 6
|Injection Volume||10-20 µl|
|Flow rate||1 ml/min|
|Column temperature||ambient (~25 °C)|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Method of Analysis||HPLC|
|Parameter||5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA)|
For the HPLC analysis of VMA, HVA and 5-HIAA in urine any isocratic HPLC system with electrochemical detector is suitable.
|Additional Info||For the HPLC analysis of VMA, HVA and 5-HIAA in urine any isocratic HPLC system with electrochemical detector is suitable.|
|Potential||approx. +760 mV|