Sample Prep Set Total Metanephrines (free + conjugated) with 96 SPE Well Plate

Order No.: 80700/96, for 96 tests
Parameters:
3-Methoxytyramine, Metanephrine, Normetanephrine

Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

3-Methoxytyramine

Metanephrine

Normetanephrine

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plate for 96 analyses (80600/96), suitable for manual and automated sample prep procedure

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of total metanephrine, normetanephrine and 3-methoxytyramine in patient urine samples by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with a suspicion of catecholamine-secreting tumours.

 

Product advantages

  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
  • Fully automated workflow solution available


This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

A fully automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (80700/C).

More Information
Number of Tests 96
Linearity

Metanephrine, Normetanephrine up to 20 mg/l
3-Methoxytyramine up to 10 mg/l

Recovery 92–107 %
Intraassay CV = 2.5–5.6 %
Interassay CV = 3.2–4.9 %
Analysis Time 4 min
Sample Preparation
  • Pipette 200 μl sample/calibrator/MassCheck® control into each well of the Hydrolysis Plate.
  • Add 50 μl Internal Standard Mix + 25 μl Hydrolysis Buffer.
  • Seal Hydrolysis Plate with pierceable adhesive seal and cover.
  • Hydrolyse in the oven for 45 min at 120 °C.
  • Cool for at least 10 min at +2 to +8 °C.
  • Place a 96 SPE Well Plate onto a Collection Plate.
  • Pipette 50 μl hydrolysate into each well of the 96 SPE Well Plate.
  • Add 600 μl Neutralisation Buffer.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Place 96 SPE Well Plate onto a new Collection Plate.
  • Pipette 500 μl Elution Buffer into each well and centrifuge 2 min at 100 x g.
  • Pipette 500 μl Dilution Buffer into each well of the Collection Plate.
  • Seal with adhesive seal.
  • Shake for 2 min at 900 rpm, transfer to autosampler.
  • Inject up to 20 μl into the LC-MS/MS system.
Sample Stability Samples are stable for at least 7 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.
Specimen 24h urine or spontaneous urine
Pre-analytic Treatment 24 h urine is collected in a suitable container with 10 ml of 10–25 % HCl.
Injection Volume ≤ 20 µl
Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 3-Methoxytyramine, Metanephrine, Normetanephrine
  1. 80703 LCMS Biogenic amines hydrolysis buffer urine
    Hydrolysis Buffer
    Order no.: 80703

    MassChrom® Total Metanephrines in Urine - LC-MS/MS

    Details
  2. 80704 LCMS Biogenic amines internal standard mix urine
    Internal Standard Mix
    Order no.: 80704

    MassChrom® Total Metanephrines in Urine - LC-MS/MS

    Details
  3. Neutralisation Buffer
    Neutralisation Buffer
    Order no.: 80605

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  4. Wash Buffer 1
    Wash Buffer 1
    Order no.: 80606

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  5. Wash Buffer 2
    Wash Buffer 2
    Order no.: 80607

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  6. Elution Buffer
    Elution Buffer
    Order no.: 80608

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  7. Dilution Buffer
    Dilution Buffer
    Order no.: 80609

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  8. 80056 LCMS Biogenic amines collection plate
    Collection Plates
    Order no.: 80056

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  9. 96 SPE Well Plate
    96 SPE Well Plate
    Order no.: 80057

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  10. 80058 LCMS Biogenic amines hydrolysis plate
    Hydrolysis Plate
    Order no.: 80058

    MassChrom® Total Metanephrines in Urine - LC-MS/MS

    Details
  11. Pierceable adhesive seals for 96 Well Plates
    Pierceable adhesive seals for 96 Well Plates
    Order no.: 80059

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  1. 80739 LCMS 6PLUS1 total metanephrines urine
    6PLUS1® Multilevel Urine Calibrator Set Total Metanephrines (free + conjugated)
    Order no.: 80739

    MassChrom® Total Metanephrines in Urine - LC-MS/MS

    Details
  1. 0376 0377 LCMS Total Metanephrines Urine Controls
    MassCheck® Total Metanephrines Urine Controls
    Order no.: 0376/0377

    MassChrom® Total Metanephrines in Urine - LC-MS/MS

    Details
  1. Tuning Mix MassChrom® Biogenic Amines/Metabolites
    Tuning Mix MassChrom® Biogenic Amines/Metabolites
    Order no.: 80615

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  2. System Check Solution
    System Check Solution
    Order no.: 80610

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  3. Cover for 96 Well Plates
    Cover for 96 Well Plates
    Order no.: 15015
     
    Details
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