MassChrom® Free Metanephrines in Plasma with Sample Clean Up Columns

Order No.: 81000/C, for 2 x 96 tests
Parameters:
3-Methoxytyramine, Metanephrine, Normetanephrine

6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
Chromatographic separation of analytes
Isotopically labelled internal standard for each analyte

Assay validated according to IVDR (=> Declaration of Conformity)

3-Methoxytyramine

Metanephrine

Normetanephrine

MassChrom® Free Metanephrines in Plasma with Sample Clean Up Columns
For 2 x 96 tests

Alternatively, there is also a method available using 96 SPE Well Plates (81000).

 

Clinical relevance

This assay allows the quantitative determination of free metanephrine, free normetanephrine and free 3-methoxytyramine in human plasma samples by LC-MS/MS. The assay can be employed as an aid to diagnose and monitor suspected pheochromocytoma or paraganglioma. Furthermore, the assay is an aid to diagnosing and monitoring suspected neuroblastoma.

 

Product advantages

• 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
• Chromatographic separation of analytes
• Isotopically labelled internal standard for each analyte

 

Assay characteristics

Sample clean-up is performed by efficient solid phase extraction either in 96 SPE well plates or SPE columns (Sample Clean Up Columns). Solid phase extraction does not require equilibration or conditioning steps and accomplishes analyte enrichment without a vaporisation step. Each analyte is measured in relation to a specific isotope-labelled internal standard to ensure that reproducible and reliable quantitative results are obtained. The 6PLUS1® Multilevel Calibrator Set and MassCheck® controls are obtained from human plasma to minimise matrix-induced differences.

Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Number of Tests 2 x 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation

Metanephrine: 2.8 ng/l – 5650 ng/l
Normetanephrine: 2.3 ng/l – 8500 ng/l
3-Methoxytyramine: 1.4 ng/l – 5800 ng/l

Different systems might show different performance data.

Specimen Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 500 µl Dilution Buffer and 25 µl Internal Standard Mix into each Sample Clean Up Column.
  • Add 500 µl of the sample/calibrator/MassCheck® control, centrifuge, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge, discard effluent.
  • Add 250 µl Elution Buffer and centrifuge.
  • Inject 5–50 µl into the LC-MS/MS system.
Run Time 5 min
Injection Volume 5 to 50 µl (mass spectrometer dependent)
Flow Rate 0.5–2.0 ml/min
Column Temperature + 25 °C
Gradient Binary
Ionisation ESI positive
MS/MS Mode MRM
Parameters 3-Methoxytyramine, Metanephrine, Normetanephrine

81000 MassChrom® Free Metanephrines in Plasma with Sample Clean Up Columns
For 2 x 96 tests

Order No. Kit components (Quantities in kit)
81001 Mobile Phase A, 750 ml (1x)
81002 Mobile Phase B, 450 ml (1x )
81003 Dilution Buffer, 50 ml (2x)
81004 Internal Standard Mix, 2 x 2,5 ml (1x)
81005 Wash Buffer 1, 90 ml (2x)
81006 Wash Buffer 2, 90 ml (4x)
81007 Elution Buffer, 25 ml (2x)
81009 Rinsing Solution, 500 ml (1x)
81055 Sample Clean Up Columns, 96 pcs. (2x)

 

Order No. Required components (not included in the kit)
81100 Analytical Column, equilibrated, with test chromatogram, 1 pc.
81039 6PLUS1® Multilevel Plasma Calibrator Set Free Metanephrines, 7 x 3.0 ml
0384 MassCheck® Free Metanephrines Plasma Control, Level I, 5 x 3.0 ml
0385 MassCheck® Free Metanephrines Plasma Control, Level II, 5 x 3.0 ml
0386 MassCheck® Free Metanephrines Plasma Control, Level III, 5 x 3.0 ml
81015 Tuning Mix, Analytes and Internal Standards, for first installation and optimisation, 1 x 1 ml

 

Order No. General lab equipment (non-CE/IVD products)
15070 Stainless Steel Prefilter Housing, 1 pc.
15071 Stainless Steel Prefilter, 0.5 μm, 5 pcs.
J0602 Autosampler Vials, screw neck, 15 μL inner cone, clear glass, 1.1 ml, 100 pcs.
J0410 PP Screw-on Caps, pierceable silicone/PTFE septa, 1.0 mm, 100 pcs.
33007 Tubes for sample preparation, 50 pcs.
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

3-Methoxytyramine

Metanephrine

Normetanephrine

MassChrom® Free Metanephrines in Plasma with Sample Clean Up Columns
For 2 x 96 tests

Alternatively, there is also a method available using 96 SPE Well Plates (81000).

 

Clinical relevance

This assay allows the quantitative determination of free metanephrine, free normetanephrine and free 3-methoxytyramine in human plasma samples by LC-MS/MS. The assay can be employed as an aid to diagnose and monitor suspected pheochromocytoma or paraganglioma. Furthermore, the assay is an aid to diagnosing and monitoring suspected neuroblastoma.

 

Product advantages

• 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
• Chromatographic separation of analytes
• Isotopically labelled internal standard for each analyte

 

Assay characteristics

Sample clean-up is performed by efficient solid phase extraction either in 96 SPE well plates or SPE columns (Sample Clean Up Columns). Solid phase extraction does not require equilibration or conditioning steps and accomplishes analyte enrichment without a vaporisation step. Each analyte is measured in relation to a specific isotope-labelled internal standard to ensure that reproducible and reliable quantitative results are obtained. The 6PLUS1® Multilevel Calibrator Set and MassCheck® controls are obtained from human plasma to minimise matrix-induced differences.

Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Number of Tests 2 x 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation

Metanephrine: 2.8 ng/l – 5650 ng/l
Normetanephrine: 2.3 ng/l – 8500 ng/l
3-Methoxytyramine: 1.4 ng/l – 5800 ng/l

Different systems might show different performance data.

Specimen Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 500 µl Dilution Buffer and 25 µl Internal Standard Mix into each Sample Clean Up Column.
  • Add 500 µl of the sample/calibrator/MassCheck® control, centrifuge, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge, discard effluent.
  • Add 250 µl Elution Buffer and centrifuge.
  • Inject 5–50 µl into the LC-MS/MS system.
Run Time 5 min
Injection Volume 5 to 50 µl (mass spectrometer dependent)
Flow Rate 0.5–2.0 ml/min
Column Temperature + 25 °C
Gradient Binary
Ionisation ESI positive
MS/MS Mode MRM
Parameters 3-Methoxytyramine, Metanephrine, Normetanephrine

81000 MassChrom® Free Metanephrines in Plasma with Sample Clean Up Columns
For 2 x 96 tests

Order No. Kit components (Quantities in kit)
81001 Mobile Phase A, 750 ml (1x)
81002 Mobile Phase B, 450 ml (1x )
81003 Dilution Buffer, 50 ml (2x)
81004 Internal Standard Mix, 2 x 2,5 ml (1x)
81005 Wash Buffer 1, 90 ml (2x)
81006 Wash Buffer 2, 90 ml (4x)
81007 Elution Buffer, 25 ml (2x)
81009 Rinsing Solution, 500 ml (1x)
81055 Sample Clean Up Columns, 96 pcs. (2x)

 

Order No. Required components (not included in the kit)
81100 Analytical Column, equilibrated, with test chromatogram, 1 pc.
81039 6PLUS1® Multilevel Plasma Calibrator Set Free Metanephrines, 7 x 3.0 ml
0384 MassCheck® Free Metanephrines Plasma Control, Level I, 5 x 3.0 ml
0385 MassCheck® Free Metanephrines Plasma Control, Level II, 5 x 3.0 ml
0386 MassCheck® Free Metanephrines Plasma Control, Level III, 5 x 3.0 ml
81015 Tuning Mix, Analytes and Internal Standards, for first installation and optimisation, 1 x 1 ml

 

Order No. General lab equipment (non-CE/IVD products)
15070 Stainless Steel Prefilter Housing, 1 pc.
15071 Stainless Steel Prefilter, 0.5 μm, 5 pcs.
J0602 Autosampler Vials, screw neck, 15 μL inner cone, clear glass, 1.1 ml, 100 pcs.
J0410 PP Screw-on Caps, pierceable silicone/PTFE septa, 1.0 mm, 100 pcs.
33007 Tubes for sample preparation, 50 pcs.
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.