MassChrom® Free Metanephrines in Plasma with Sample Clean Up Columns

MassChrom® Free Metanephrines in Plasma
With Sample Clean Up Columns, for manual sample preparation
For 2 x 96 tests

Clinical relevance

This assay allows the quantitative determination of metanephrine, normetanephrine and 3-methoxytyramine in plasma by LC-MS/MS.

One typical application is the analysis of patient samples with a suspicion of pheochromocytomas. These tumours and other neural crest tumours (e.g. paragangliomas and neuroblastomas) secrete metanephrine, normetanephrine and 3-methoxytyramine. The plasma free metanephrines are more stable and secreted more evenly than catecholamines (epinephrine, norepinephrine, and dopamine). Some studies show that the analysis of plasma free metanephrines has the highest sensitivity and specificity in pheochromocytoma diagnostics compared to plasma free catecholamines and metanephrines in urine.

Product advantages

• 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
• Simple sample prep in just a few steps
• Chromatographic separation of analytes – no co-elution
• Isotopically labelled internal standards for all analytes

The assay encompasses all required components and allows a simple and fast sample preparation: solid phase extraction (SPE) without equilibration/conditioning steps, analyte enrichment without evaporation step, only one elution step. The analytes are chromatographically separated to avoid co-elution and to minimise risks of interferences. The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantification. The 6PLUS1® Multilevel Plasma Calibrator Set and MassCheck® controls ensure high precision of results. They are obtained from human plasma to minimise matrix-induced differences.

There is also a method using 96 SPE Well Plates (81000) as well as the corresponding fully automated workflow solution available for laboratories with a higher sample throughput.