MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate

Order No.: 81000, for 2 x 96 tests
Parameters:
3-Methoxytyramine, Metanephrine, Normetanephrine

6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
Isotopically labelled internal standard for each analyte
Fully automated workflow solution available

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

3-Methoxytyramine

Metanephrine

Normetanephrine

MassChrom® Free Metanephrines in Plasma
With 96 SPE Well Plates, suitable for manual and automated sample preparation
For 2 x 96 tests

 

Clinical relevance

This assay allows the quantitative determination of metanephrine, normetanephrine and 3-methoxytyramine in plasma by LC-MS/MS.

One typical application is the analysis of patient samples with a suspicion of pheochromocytomas. These tumours and other neural crest tumours (e.g. paragangliomas and neuroblastomas) secrete metanephrine, normetanephrine and 3-methoxytyramine. The plasma free metanephrines are more stable and secreted more evenly than catecholamines (epinephrine, norepinephrine, and dopamine). Some studies show that the analysis of plasma free metanephrines has the highest sensitivity and specificity in pheochromocytoma diagnostics compared to plasma free catecholamines and metanephrines in urine.

 

Product advantages

  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
  • Simple sample prep in just a few steps
  • Chromatographic separation of analytes – no co-elution
  • Isotopically labelled internal standards for all analytes
  • Fully automated workflow solution available

The assay encompasses all required components and allows a simple and fast sample preparation: solid phase extraction (SPE) without equilibration/conditioning steps, analyte enrichment without evaporation step, only one elution step. The analytes are chromatographically separated to avoid co-elution and to minimise risks of interferences. The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantification. The 6PLUS1® Multilevel Plasma Calibrator Set and MassCheck® controls ensure high precision of results. They are obtained from human plasma to minimise matrix-induced differences.

A fully automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (81000/C).

More Information
Number of Tests 2 x 96
Limit of quantification

Metanephrine: 15 ng/l
Normetanephrine: 7 ng/l
3-Methoxytyramine: 11 ng/l

Linearity

Metanephrine: up to 5650 ng/l
Normetanephrine: up to 8500 ng/l
3-Methoxytyramine: up to 5800 ng/l

Recovery 89–100 %
Intraassay CV = 2.7–6.9 %
Interassay CV = 3.3–8.6 %
Analysis Time 5 min
Sample Preparation
  • Pipette 500 µl Dilution Buffer and 25 µl Internal Standard Mix into each well of the 96 SPE Well Plate.
  • Add 500 µl of the sample/calibrator/MassCheck® control, centrifuge, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge, discard effluent.
  • Add 250 µl Elution Buffer into each well and centrifuge.
  • Inject up to 25 µl.
Specimen Plasma
Injection Volume ≤ 25 µl
Gradient Binary
Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 3-Methoxytyramine, Metanephrine, Normetanephrine
  1. Mobile Phase A
    Mobile Phase A
    Order no.: 81001

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  2. Mobile Phase B
    Mobile Phase B
    Order no.: 81002

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  3. Dilution Buffer
    Dilution Buffer
    Order no.: 81003

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  4. Internal Standard Mix
    Internal Standard Mix
    Order no.: 81004

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  5. Wash Buffer 1
    Wash Buffer 1
    Order no.: 81005

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  6. Wash Buffer 2
    Wash Buffer 2
    Order no.: 81006

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  7. Elution Buffer
    Elution Buffer
    Order no.: 81007

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  8. Rinsing Solution
    Rinsing Solution
    Order no.: 81009

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  9. 96 SPE Well Plate
    96 SPE Well Plate
    Order no.: 81057

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  10. Collection Plates
    Collection Plates
    Order no.: 81058

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
  11. Pierceable adhesive seals for 96 Well Plates
    Pierceable adhesive seals for 96 Well Plates
    Order no.: 81059

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

    Details
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    6PLUS1® Multilevel Plasma Calibrator Set Free Metanephrines
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    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

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  1. 0384 0385 0386 LCMS metanephrines plasma controls
    MassCheck® Free Metanephrines Plasma Controls
    Order no.: 0384/0385/0386

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

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  1. 81100 LCMS column metanephrines plasma
    Analytical Column Free Metanephrines in Plasma - LC-MS/MS
    Order no.: 81100

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

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  2. 81015 tuning mix free metanephrines plasma
    Tuning Mix Free Metanephrines in Plasma
    Order no.: 81015

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

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  3. 81010 system check solution free metanephrines plasma
    System Check Solution
    Order no.: 81010

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

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  4. 80200 biogenic amines analytical column VMA HVA 5-HIAA
    Waste Plates
    Order no.: 81056

    MassChrom® Free Metanephrines in Plasma - LC-MS/MS

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