Automated Vitamins A and E in Serum/Plasma - UHPLC
Automated sample preparation with 96 well filter plates
96 samples in less than 30 minutes
Works on a range of liquid handling devices
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
Vitamin A
Vitamin E
Clinical relevance
Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.
Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.
Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.
However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.
Product advantages
- Automated sample preparation:
96 samples in less than 30 minutes
This UHPLC kit allows for the automated analysis of vitamins A and E in plasma/serum. The automated preparation of 96 samples can be completed in less than 30 minutes – ideal for resource-saving processing of larger numbers of samples. The UHPLC determination is performed in less than 4 minutes.
Chromsystems also offers another automated method using HPLC.
Content for 10 x 96 tests
Mobile Phase, 2 x 500 ml
Precipitation Reagent I, 4 x 2.5 ml
Internal Standard, 2 x 100 ml
Vitamins A and E Serum Calibration Standard (lyoph.), 5 x 1 ml
96 Well Filter Plates, 2 x 5 pcs.
96 Well Collection Plates, 2 x 5 pcs.
Pierceable Heat Seals for 96 Well Plates, 2 x 6 pcs.
UHPLC Column, 1 pc.
UHPLC Precolumn Cartridge, 3 pcs.
| Method of Analysis | UHPLC |
|---|---|
| Number of Tests | 10 x 96 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | Vitamin A: 0.02 mg/l |
| Upper Limit of Quantification | Vitamin A: 0.02-4.5 mg/l Vitamin E: 0.4-38 mg/l |
| Intraassay | Vitamin A: CV < 5 % |
| Interassay | Vitamin A: CV < 5 % |
| Recovery | Vitamin A > 94 %; Vitamin E > 90 % |
| Specimen | Serum/Plasma |
| Sample Preparation |
|
| Run Time | 3.5 min |
| Injection Volume | 5 µl |
| Column Temperature | approx. 35°C |
| Wavelengths | 325 nm, after 1.3 min switch to 295 nm |
| Parameters | Vitamin A (Retinol), Vitamin E (α-Tocopherol) |
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC -
-
-
Pierceable Heat Seals for 96 Well PlatesOrder no.: 34760Optional, as an alternative to 34459 (Cross-slitted Adhesive Seals)
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
-
Chromsystems Heat SealerOrder no.: 42740Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint
Vitamin A
Vitamin E
Clinical relevance
Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.
Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.
Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.
However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.
Product advantages
- Automated sample preparation:
96 samples in less than 30 minutes
This UHPLC kit allows for the automated analysis of vitamins A and E in plasma/serum. The automated preparation of 96 samples can be completed in less than 30 minutes – ideal for resource-saving processing of larger numbers of samples. The UHPLC determination is performed in less than 4 minutes.
Chromsystems also offers another automated method using HPLC.
Content for 10 x 96 tests
Mobile Phase, 2 x 500 ml
Precipitation Reagent I, 4 x 2.5 ml
Internal Standard, 2 x 100 ml
Vitamins A and E Serum Calibration Standard (lyoph.), 5 x 1 ml
96 Well Filter Plates, 2 x 5 pcs.
96 Well Collection Plates, 2 x 5 pcs.
Pierceable Heat Seals for 96 Well Plates, 2 x 6 pcs.
UHPLC Column, 1 pc.
UHPLC Precolumn Cartridge, 3 pcs.
| Method of Analysis | UHPLC |
|---|---|
| Number of Tests | 10 x 96 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | Vitamin A: 0.02 mg/l |
| Upper Limit of Quantification | Vitamin A: 0.02-4.5 mg/l Vitamin E: 0.4-38 mg/l |
| Intraassay | Vitamin A: CV < 5 % |
| Interassay | Vitamin A: CV < 5 % |
| Recovery | Vitamin A > 94 %; Vitamin E > 90 % |
| Specimen | Serum/Plasma |
| Sample Preparation |
|
| Run Time | 3.5 min |
| Injection Volume | 5 µl |
| Column Temperature | approx. 35°C |
| Wavelengths | 325 nm, after 1.3 min switch to 295 nm |
| Parameters | Vitamin A (Retinol), Vitamin E (α-Tocopherol) |
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
-
-
-
Pierceable Heat Seals for 96 Well PlatesOrder no.: 34760Optional, as an alternative to 34459 (Cross-slitted Adhesive Seals)
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
-
Chromsystems Heat SealerOrder no.: 42740
Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint