Sample Prep Set VMA, HVA, 5-HIAA with Autosampler Vials
Simple and fast sample prep
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
5-Hydroxyindoleacetic Acid (5-HIAA)
Homovanillic Acid (HVA)
Vanillylmandelic Acid (VMA)
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With autosampler vials, for manual sample preparation
Clinical Relevance
This Sample Prep Set allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS. It is used for the diagnostics of neuroblastoma and carcinoid tumours.
Product advantage
- Simple and fast sample prep consisting of 2 pipetting steps and subsequent injection
- Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.
There is also a method using collection plates (80800/96 & 80800/480) as well as the corresponding fully automated workflow solution available for laboratories with a higher sample throughput.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 |
| Limit of quantification | 0.2 - 2.6 mg/l |
| Linearity | VMA, 5-HIAA up to 150 mg/l |
| Recovery | 95 - 113 % |
| Intraassay | CV 2.5 - 6.7 % |
| Interassay | CV 3.7 - 7.7 % |
| Analysis Time | 3 min |
| Specimen | 24h urine or spontaneous urine |
| Pre-analytic Treatment | 24 h urine is collected in a suitable container with 10 ml of 10–25 % HCl (VMA, HVA) or 10 ml glacial acetic acid (5-HIAA). Patient specimens are stable for up to 14 days at +2 to +8 °C in amber coloured vials. |
| Sample Preparation |
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| Sample Stability | The prepared samples are stable for up to 5 days (VMA and HVA) or up to 3 days ( 5-HIAA) at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C. |
| Injection Volume | ≤ 2 µl |
| Column temperature | +25 °C |
| Ionisation | ESI positive |
| MS/MS-Mode | MRM |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Parameter | 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA) |
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6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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System Check Solution SetOrder no.: 80810MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Tuning Mix VMA, HVA, 5-HIAAOrder no.: 80815MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Analytical Column VMA, HVA, 5-HIAA Urine - LC-MS/MSOrder no.: 80200MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
