Parameter Set Antiepileptic Drugs - LC-MS/MS

Order No.: 92921/RUO
Parameters:
10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

Encompasses 26 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

Carbamazepine

Carbamazepine-10,11-epoxide

10,11-Dihydroxycarbamazepine

10-OH-Carbamazepine

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam (Keppra®)

N-Desmethylmesuximide

Oxcarbazepine

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

Stiripentol

Sultiame

Theophyllie

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamid

Clinical relevance

This parameter set allows the quantitative determination of 26 analytes in human serum or plasma samples via liquid chromatography mass spectrometry (LC-MS/MS).

 

MassTox® Series A

The MassTox® Series A is a modular system that enables the determination of all analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® Basic Kit A
• Specific MassTox® Parameter Set
• Analytical column MassTox® TDM MasterColumn® A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min
  • Dilute supernatant with Dilution Buffer prior to injection, depending on instrument sensitivity.
Run Time 2.5 – 3.5 min
Injection Volume 0.2 – 50 µl
Gradient

Starting point: 0% Mobile Phase 2

Group 1
0.00→2.00 min, 100 % Mobile Phase 2
2.00-2.60 min, 100 % Mobile Phase 2
2.61-3.50 min, 0 % Mobile Phase 2

Group 2 and 3
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.60 min, 100 % Mobile Phase 2
1.61-2.50 min, 0 % Mobile Phase 2

Group 4 and 5
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.80 min, 100 % Mobile Phase 2
1.81-2.70 min, 0 % Mobile Phase 2

Ionisation ESI positive and negative
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters 10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:

Carbamazepine

Carbamazepine-10,11-epoxide

10,11-Dihydroxycarbamazepine

10-OH-Carbamazepine

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam (Keppra®)

N-Desmethylmesuximide

Oxcarbazepine

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

Stiripentol

Sultiame

Theophyllie

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamid

Clinical relevance

This parameter set allows the quantitative determination of 26 analytes in human serum or plasma samples via liquid chromatography mass spectrometry (LC-MS/MS).

 

MassTox® Series A

The MassTox® Series A is a modular system that enables the determination of all analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® Basic Kit A
• Specific MassTox® Parameter Set
• Analytical column MassTox® TDM MasterColumn® A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min
  • Dilute supernatant with Dilution Buffer prior to injection, depending on instrument sensitivity.
Run Time 2.5 – 3.5 min
Injection Volume 0.2 – 50 µl
Gradient

Starting point: 0% Mobile Phase 2

Group 1
0.00→2.00 min, 100 % Mobile Phase 2
2.00-2.60 min, 100 % Mobile Phase 2
2.61-3.50 min, 0 % Mobile Phase 2

Group 2 and 3
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.60 min, 100 % Mobile Phase 2
1.61-2.50 min, 0 % Mobile Phase 2

Group 4 and 5
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.80 min, 100 % Mobile Phase 2
1.81-2.70 min, 0 % Mobile Phase 2

Ionisation ESI positive and negative
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters 10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method: