Parameter Set Neuroleptics 1/EXTENDED - LC-MS/MS

Order No.: 92912/XT/RUO
Parameters:
9-OH-Risperidone, Aripiprazole, Clozapine, Haloperidol, N-Desmethylclozapine, N-Desmethylolanzapine, Olanzapine, Quetiapine, Risperidone

Encompasses 11 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

 

Aripiprazole

Dehydroaripiprazole

Clozapine

N-Desmethylclozapine

Haloperidol

Olanzapine

N-Desmethylolanzapine

Quetiapine

Norquetiapine

Risperidone

9-OH-Risperidone

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Neuroleptics 1/EXTENDED allows for the fast quantitative determination of aripiprazole, dehydroaripiprazole, clozapine, N-desmethylclozapine, haloperidol, olanzapine, N-desmethylolanzapine, quetiapine, norquetiapine, risperidone and 9-OH-risperidone in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

 

More Information
Analysis Time 3 min
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Sample Stability Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.
Specimen Serum/Plasma
Injection Volume 0.2 – 50 µl
Gradient

0.00 - 0.20 min    20 % Mobile Phase 2
0.21 - 0.70 min    50 % Mobile Phase 2
0.71 - 2.30 min    70 % Mobile Phase 2
2.31 - 3.00 min    20 % Mobile Phase 2

Ionisation ESI
MS/MS-Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 9-OH-Risperidone, Aripiprazole, Clozapine, Haloperidol, N-Desmethylclozapine, N-Desmethylolanzapine, Olanzapine, Quetiapine, Risperidone
  1. 0210XT 0211XT 0212XT TDM Series A contols neuroleptics1_XT
    Details
  2. Internal Standard Set Antidepressants 1/EXTENDED and Neuroleptics 1/EXTENDED
    Internal Standard Set Antidepressants 1/EXTENDED and Neuroleptics 1/EXTENDED
    Order no.: 92046/AN1/XT/RUO

    Component of the Parameter Set Antidepressants 1/EXTENDED and Neuroleptics 1/EXTENDED, available separately

    Details
  1. 0210XT 0211XT 0212XT TDM Series A contols neuroleptics1_XT
    Details
  1. 92110 TDM Series A Master Column
    MassTox® MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A

    Details
  2. Tuning Mix Neuroleptics 1/EXTENDED
    Tuning Mix Neuroleptics 1/EXTENDED
    Order no.: 92015/N1/XT/RUO

    Tuning Mix for Parameter Set Neuroleptics 1/EXTENDED

    Details
  3. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  4. PEEK-encased Prefilter, 2 µm
    Details