Parameter Set Mycophenolic Acid - LC-MS/MS

Order No.: 92916/RUO
Parameters:

For the analysis of MPA and MPAG
6PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

Mycophenolic Acid

Mycophenolic Acid Glucuronide

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Mycophenolic Acid allows for the fast quantitative determination of mycophenolic acid and its glucuronide in serum/plasma by LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrators ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

More Information
Analysis Time 1.8 min
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Sample Stability Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.
Specimen Serum/Plasma
Injection Volume 0.2 – 50 µl
Gradient

Starting point       60 % Mobile Phase 2
0.00→0.50 min    100 % Mobile Phase 2
0.51–1.00 min    100 % Mobile Phase 2
1.01→1.10 min     60 % Mobile Phase 2
1.11–1.81 min      60 % Mobile Phase 2

Ionisation ESI positive
MS/MS-Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
  1. Internal Standard Set Mycophenolic Acid/Glucuronide
    Internal Standard Set Mycophenolic Acid/Glucuronide
    Order no.: 92246/RUO
    Component of the Parameter Set Mycophenolic Acid/Glucuronide, available separately
    Details
  1. 92110 TDM Series A Master Column
    MassTox® MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A

    Details
  2. Tuning Mix Mycophenolic Acid/Glucuronide
    Tuning Mix Mycophenolic Acid/Glucuronide
    Order no.: 92019/RUO
    Tuning Mix for the Parameter Set Mycophenolic Acid/Glucuronide - LC-MS/MS
    Details
  3. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  4. PEEK-encased Prefilter, 2 µm
    Details