Parameter Set Antimycotic Drugs/EXTENDED - LC-MS/MS

Order No.: 92922/XT/RUO
Parameters:
Amphotericin B, Anidulafungin, Caspofungin, Fluconazole, Hydroxyitraconazole, Isavuconazole, Itraconazole, Ketoconazole, Micafungin, Posaconazole, Voriconazole

Extended parameter menu of now 12 analytes
Excellent stability of all componentes
Part of the modular system MassTox® Series A

5-Flucytosine

Amphotericin B

Anidulafungin

Caspofungin

Fluconazole

Isavuconazole

Itraconazole

Hydroxyitraconazole

Ketoconazole

Micafungin

Posaconazole

Voriconazole

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set Antimycotic Drugs allows for the fast quantitative determination of 12 different analytes in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

More Information
Sample Preparation
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A.
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A, mix 30 s (vortex)and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity.
Sample Stability Stability of research samples depends on the specific analyte. Further information can be obtained from the instruction manual.
Specimen Serum/Plasma
Injection Volume 1–20 μl
Column temperature ambient (≈ 25 °C)
Gradient binary
Ionisation ESI positive and negative, polarity switch possible
MS/MS-Mode MRM
Additional Info It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Amphotericin B, Anidulafungin, Caspofungin, Fluconazole, Hydroxyitraconazole, Isavuconazole, Itraconazole, Ketoconazole, Micafungin, Posaconazole, Voriconazole
  1. 0252 0253 0254 LCMS TDM Series A controls antimycotic drugs
    Details
  2. Internal Standard Mix Antimycotic Drugs/EXTENDED
    Internal Standard Mix Antimycotic Drugs/EXTENDED
    Order no.: 92644/XT/RUO
    Component of the Parameter Set Antimycotic Drugs/EXTENDED
    Details
  1. 0252 0253 0254 LCMS TDM Series A controls antimycotic drugs
    Details
  1. 92110 TDM Series A Master Column
    MassTox® MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A

    Details
  2. Tuning Mix Antimycotic Drugs/EXTENDED
    Tuning Mix Antimycotic Drugs/EXTENDED
    Order no.: 92039/XT/RUO
    Tuning Mix for the Parameter Set Antimycotic Drugs/EXTENDED - LC-MS/MS
    Details
  3. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  4. PEEK-encased Prefilter, 2 µm
    Details